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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of the fragmented total RNA per sample was used for RNA immunoprecipitation (IP) with 5 µg of the anti-m6A antibody (ABE572, Millipore). RNA-seq libraries from input and IP samples were prepared using the SMARTer Pico Input Mammalian v2 RNA-seq kit (Takara ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Nonspecific binding was blocked with 5% normal goat serum and 0.05% saponin (Sigma) in PBS (pH 7.4 ...
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bioRxiv - Systems Biology 2021Quote: ... Unspecific binding sites were blocked using 5% bovine serum albumin (BSA) (Sigma Aldrich) in PBS including 0.3% Triton X-100 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... DPBS and Annexin V binding buffer were filtered through 0.1-μm filters (Millipore). Flow cytometry was performed using a FACSVerse™ flow cytometer (BD Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Unspecific binding was subsequently blocked using 1% bovine serum albumin (A9418, Sigma-Aldrich) for 1 hour ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... 8 ml of binding buffer [PBS-2 mM EDTA-2% BSA (Sigma Aldrich) (w/v)] were added and the mix was passed through a 40 µm filter (Avantor) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed 2 times and permeabilized in binding buffer (0.1% saponin (Sigma) and 0.2% BSA (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... in pre-silanised non-binding tubes (Repel-silane ES, Sigma, 17-1332-01). The reaction mix (94 µl per well ...
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bioRxiv - Cancer Biology 2024Quote: ... After blocking nonspecific binding sites for 1 h using 5% dry milk (Sigma) in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2022Quote: ... followed by a protospacer adjacent motif [PAM] of ‘NGG’) were selected using the prediction software (www.crispr.mit.edu) and synthesised by Sigma, annealed and ligated into the pX330-U6-Chimeric-BB-CBh-hSpCas9 plasmid (Addgene 42230) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-protein complexes were retrieved from the cleared lysate on anti-Flag M2 beads (Sigma Aldrich) and eluted using 3x Flag peptide ...
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bioRxiv - Cancer Biology 2020Quote: ... S9.6 antibody that binds to RNA/DNA hybrid was purchased from Millipore (MABE1095).
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bioRxiv - Biochemistry 2022Quote: ... and the anti-RNA polymerase II antibody clone CTD4H8 (Millipore, cat# 05-623). Note that H3K9me2 antibody (mAbProtein ...
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bioRxiv - Molecular Biology 2020Quote: ... The antibodies used in this study were: mouse anti-RNA polymerase II (Millipore, 05-623 ...
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bioRxiv - Microbiology 2020Quote: ... A region of pol (44, 45) was amplified using KOD Hot Start Master Mix (Millipore, 71842) in a first round of PCR with forward primer ...
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bioRxiv - Biochemistry 2021Quote: ... for 45 minutes at 37 °C and subsequently alkylated with 20 mM iodoacetamide (IAA, Sigma Aldrich) at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... and calf thymus histones (45 mg/Kg in sterile saline; Roche® distributed by Sigma-Millipore; #10223565001 ...
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bioRxiv - Microbiology 2021Quote: ... at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich) for 15 min at 25 °C in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... was warmed at 45 °C and mixed with PDMS and SYLGARD 184 curing agent (761036, Sigma) at the weight ratio of 50:10:1 ...
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bioRxiv - Physiology 2022Quote: ... Stained slides were washed and counterstained for 30-45 seconds with Gill’s 3 Hematoxylin (Sigma Aldrich), then rinsed thoroughly with water and mounted with glycerol gelatin (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... 45 µg of nuclei were digested with 0.75 µg of Nuclease S7 (Sigma-Aldrich, Cat#10107921001) for 15 min at 37 °C in 100 µl of a buffer containing 20 mM of Tris-HCl pH7.5 ...
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bioRxiv - Microbiology 2023Quote: ... and treated with 3 × 45 s bead beating with glass beads (particle size ≤106 μm, Sigma) at maximum power and cooling on ice between cycles (MP Biomedicals™ FastPrep- 24TM ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated for 45 minutes with 1 μg/ml of FITC-phalloidin (Sigma Aldrich, Italy), to stain actin cytoskeleton ...
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bioRxiv - Systems Biology 2023Quote: ... at 55 °C for 45 min and then alkylated with 55 mM iodoacetamide (Sigma Aldrich, A3221) in the dark at room temperature for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... samples were treated at 37°C for 45 minutes with 0.03% proteinase K (Sigma, #P2308-10MG) in pre-warmed PBS to promote digestion of the superficial layer of larvae and prepupae ...
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bioRxiv - Microbiology 2023Quote: ... The photosynthetic medium (PM) (45) supplemented with 1% sodium caseinate (Sigma Aldrich, St. Louis, MO, USA) as the sole carbon source (PM-casein medium ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated with primary antibody overnight at 4 °C [1:500 goat anti-RNA polymerase II antibody (PLA0292, Sigma) with 1:500 rabbit anti-PCNA antibody (PLA0079 ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for ChIP analysis: Mouse anti-RNA polymerase II antibody clone CTD4H8 (Merck Millipore, 05-623), Rabbit anti-NF-kB p65 antibody clone D14E12 (Cell Signalling ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies against cytoskeletal proteins (vimentin, actin and β-tubulin were from Sigma (St.Louis, MO, USA). The mitochondrial marker MitoTracker® ...
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bioRxiv - Microbiology 2019Quote: ... Immunoblotting was performed with anti-Protein A mouse monoclonal primary antibody (SPA-27, Sigma Aldrich) or anti-LukS-PV mouse monoclonal primary antibody (IBT) ...
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bioRxiv - Plant Biology 2019Quote: ... Immunoblots of targeted proteins were visualized using HRP-conjugated secondary antibodies (Catalog No. AP187P, Millipore) with SuperSignal™ West Femto Maximum Sensitivity Substrate (Catalog No ...
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bioRxiv - Genetics 2020Quote: ... Chromatin-bound antibodies were recovered with 30μl Protein A/G magnetic beads (Millipore 16-663). After reverse crosslinking ...
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bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibody against HCoV-OC43 nucleocapsid (NP) protein was purchased from Millipore (Cat.No. MAB9012). Rabbit polyclonal antibody against human LY6E was obtained from proteintech (Cat.No ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies (listed in Table S4) were pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Microbiology 2021Quote: ... was used for blotting and proteins were stained using the following antibodies: (Sigma-Aldrich AV46863), TMEM41B (Sigma-Aldrich HPA014946) ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein-antibody complex was visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Neuroscience 2019Quote: Antibodies used were: rat anti-myelin basic protein (MBP, 1:200, MAB386, Millipore, MA, USA), rabbit anti-Olig2 (1:200 ...
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bioRxiv - Systems Biology 2019Quote: ... Expression of the CARD-CC proteins was determined with anti-Flag antibody (F-3165, Sigma). Expression of the E-tagged PKC clones with anti-E-tag antibody (ab66152 ...
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bioRxiv - Molecular Biology 2021Quote: ... The following commercially available antibodies were used to detect targeted proteins: anti-SPF45 (Sigma-Aldrich), anti-SF3b155 (MBL Life Science) ...
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
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bioRxiv - Microbiology 2021Quote: ... MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I, Merck Millipore, France).
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bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.
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bioRxiv - Cell Biology 2020Quote: The following proteins were detected by western blotting (antibodies in brackets) CPSF3 (Sigma-Aldrich, HPA04657), IntS11 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used for protein immunoblots: Anti p-Tyr (4G10, Millipore Sigma, #05-321 and P-Tyr-1000 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein glycosylation status was analysed by Western Blot using an anti-c-myc antibody (Sigma), anti-rabbit IgG-peroxidase conjugated (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... The FLAG M2 antibody used for the detection of MetRSL274G protein was purchase from Sigma. ANL ...