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Citations for Millipore Sigma :
701 - 750 of 3788 citations for Human Ephrin B2 EFNB2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Wild-type and mutant VP40 bearing a Strep-Tag were cloned into pTriEx-5 (Novagen) and transfected into cells using TrasnIT-LT1 transfection reagent (Mirus) ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary anti-Flag tag antibody was obtained from Sigma-Aldrich (F7425-.2MG, WB 1:2000). Primary antibodies against viral proteins were obtained from ...
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bioRxiv - Microbiology 2022Quote: ... the primary antibodies were horse radish peroxidase-conjugated α-poly-histidine tag antibody (Sigma Aldrich; St ...
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bioRxiv - Developmental Biology 2019Quote: ... and the histidine tag removed with carboxypeptidase A bound to agarose (Sigma, St. Louis, MO) at a ratio of 10 protein:1 enzyme (w/w) ...
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bioRxiv - Biophysics 2022Quote: ... the N-terminal hexa-histidine tag was cleaved with ∼2 Units of bovine thrombin (Sigma) per 1 mg of protein ...
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bioRxiv - Molecular Biology 2023Quote: ... the sequence containing the HA tag were transferred in a bacterial expression vector pET22b (Novagen) between Xho1 and HindIII restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... CUT&Tag analysis was assayed for the following epigenetic markers: H3K4Me2 (Millipore, Cat# 07-030), H3K27Ac (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... the histidine tag was then removed by proteolytic digestion with 1U/mg of thrombin (SIGMA). The digestion mixture was then loaded again on the IMAC on a Ni2+-His-Trap column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... full-length REC114 and mutants were cloned as Strep-tag fusions into pRSFDuet-1 (Novagen). MEI41-43 and its mutants were cloned as His-MBP fusions in pETM41 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma) at the 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated with a rabbit anti-HA-tag antibody (H6908, Sigma‒Aldrich) in PBS-Tx supplemented with 0.2% BSA ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with M2 antibody (anti-FLAG-tag, catalogue no. F1804A-5MG; Sigma) at 4 ℃ for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... 68] (dilution 1:1000) and mouse mAb to FLAG tag (Sigma, F3165, dilution 1:500).
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG tag antibody (1:1000 dilution used) raised in mouse (F1804) was obtained from Sigma-Aldrich and Porin antibody (1:5000 dilution used ...
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bioRxiv - Microbiology 2020Quote: Antibodies against LeishIF4E2(rabbit polyclonal, 1:2,000) and against the SBP tag (Millipore, monoclonal, 1:10,000), were used to detect the endogenous and tagged LeishIF4E2 proteins ...
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bioRxiv - Microbiology 2019Quote: ... The hexa-histidine tag was removed overnight with thrombin (5 U thrombin (Sigma-Aldrich, Darmstadt, Germany) per mg SiiA protein ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...
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bioRxiv - Biochemistry 2021Quote: Monoclonal antibodies against the Flag tag epitope and Cdh1 were purchased from Sigma (St. Louis, MO) while antibodies against GFP ...
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bioRxiv - Biophysics 2020Quote: ... MSP1D1 was purified using IMAC72 with further cleavage of 6xHis-tag by TEV protease (Sigma-Aldrich). The lipid mixture of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) ...
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bioRxiv - Molecular Biology 2022Quote: Full-length REC114 and its variants were cloned as Strep-tag fusions into pRSFDeut-1 (Novagen). TOPOVIBL 1-385 was cloned as a 6xHis-SUMO fusion protein in pETM11 ...
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bioRxiv - Biochemistry 2019Quote: ... The hexahistidine tag was removed by thrombin cleavage by incubating with 1:5000 thrombin (EMD Millipore) overnight at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... They included Fluidigm CS1 and CS2 tags and were ordered from Sigma (see appendix table 3). Reactions were performed in multiplex primer groups with primers each at 1 μM concentration (group 1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Orphan GPCR expression was detected using rat anti-HA tag (clone 3F10) antibodies (Sigma-Aldrich; 11867423001) or rabbit anti-myc tag antibodies (GenScript ...
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bioRxiv - Cell Biology 2021Quote: The flag-tag immunoprecipitations were carried out using FLAG-M2 coated agarose beads (Sigma-Aldrich #F2426) for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Microbiology 2022Quote: ... followed by incubation with primary antibodies against Strep Tag II Antibody HRP conjugate (Sigma, 1:5000), anti-FLAG Antibody (Sigma F7425 ...
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bioRxiv - Microbiology 2023Quote: ... ORF3c-HA was detected using a primary antibody against the HA-tag (Sigma-Aldrich, Cat# H3663) followed by staining with a secondary AF555 antibody (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Flag-Tag (F1804, 1:2,000 for Western blot, 1:200 for immunofluorescence) was from Sigma-Aldrich. Anti-mouse IgG–HRP (7076S ...
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bioRxiv - Cell Biology 2023Quote: ... HA tag sequence (YPYDVPDYA) was introduced by linker insertion at the 5’ side of pET28a (Novagen) MCS to generate pET28-HA-Nter ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA tag-PLUS PLA probe and anti-rabbit IgG-MINUS PLA probes (DUO92005: Sigma-Aldrich) appropriately diluted in the Duolink antibody diluent were applied onto the specimens that were incubated for 60 min at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were probed with primary antibodies against FLAG-tag (dilution 1:1000, mouse; Sigma, A1804), Wee1 (dilution 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Western blotting for the 6xHis-tag was performed using either HRP-conjugated primary antibody (Sigma, rabbit) or non-HRP-conjugated antisera (rabbit).
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bioRxiv - Microbiology 2024Quote: ... and incubated with a mouse monoclonal antibody [M2] specific to the FLAG-tag (Sigma-Aldrich, F1804) in 1% BSA/PBS at 37℃ for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...