Labshake search
Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Galectin 1 LGALS1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... with 2% human serum (Sigma), then labelled with APC-conjugated anti-mouse CD31 antibody ...
-
bioRxiv - Immunology 2023Quote: ... +5% human AB serum (Sigma) +2 mM L-glutamine +100 units penicillin-streptomycin +25 mM HEPES buffer +1x non-essential amino acids (NEAA) ...
-
bioRxiv - Cell Biology 2023Quote: ... Human Hemoglobin (Hb) (H7379, Sigma) was solubilized in sterile H2O at 10 mg/mL concentration ...
-
bioRxiv - Bioengineering 2023Quote: ... and Thrombin (Human plasma, Sigma). Type I collagen (Fisher ...
-
bioRxiv - Cell Biology 2023Quote: ... human plasma (Sigma-Aldrich, #5051); Rhodamine-conjugated fibronectin (Cytoskeleton Inc. ...
-
bioRxiv - Immunology 2023Quote: ... A human IgG (Sigma #I2511) standard curve (ten three-fold serial dilutions starting at 20 µg/ml ...
-
bioRxiv - Immunology 2023Quote: ... 2% human serum (Sigma-Aldrich), 1% non-essential amino acids (ThermoFisher) ...
-
bioRxiv - Cell Biology 2023Quote: ... 850 nM human insulin (Sigma), 500 nM 3-isobutyl-1-methylxanthine (IBMX ...
-
bioRxiv - Cell Biology 2023Quote: ... 850 nM human insulin (Sigma), 500 nM IBMX (Sigma) ...
-
bioRxiv - Immunology 2024Quote: ... A human IgG (Sigma #I2511) standard curve (10 three-fold serial dilutions starting at 20 µg/mL ...
-
bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
-
bioRxiv - Microbiology 2021Quote: ... in PBS for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) for 1 hour at room temperature ...
-
bioRxiv - Microbiology 2019Quote: ... Routine plaque assays were performed in duplicate in the presence of 1% pooled neutralising human serum (Sigma). Single-step infections were performed at moi 5 or 10 pfu/cell and multi-round infections at a moi of 0.005 pfu/cell ...
-
bioRxiv - Cancer Biology 2021Quote: ... and then incubated overnight at 4 ℃ with primary antibodies against human Kv1.3 (1: 1000, P4497, Sigma, USA), P-p38 MAPK (1 ...
-
bioRxiv - Cell Biology 2022Quote: ... They were then overlaid with 10-15 μl of 1 mg/ml Human IgG (Millipore Sigma, I4506) mixed with either 20 μg/ml Goat anti-Mouse IgG Alexa Fluor 405 (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... and binding detected using 1 in 25,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich).
-
bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
-
bioRxiv - Genomics 2019Quote: The MISSION pLKO.1-puro human TDP-43 (TRCN0000016038) and control shRNAs (Sigma Aldrich SHC007 and SHC016) were used to produce lentivirus ...
-
bioRxiv - Microbiology 2021Quote: ... of human DDX21 isoform 1 (NM_004728.4) and isoform 2 (NM_001256910.1) into plasmid p3XFLAG-CMV-14 (Sigma-Aldrich), respectively ...
-
bioRxiv - Immunology 2020Quote: ... Human monocytic cell line (THP-1) cells were differentiated with 100 nM phorbol myristate acetate (Sigma-Aldrich) for 3 days and then the same polarization protocol was performed ...
-
bioRxiv - Molecular Biology 2023Quote: ... EpiLC transition was induced by plating 1×103 naive mESCs on a human plasma fibronectin (Millipore, FC010) coated plate in N2B27 medium supplemented with 20 ng/mL activin A (SCI) ...
-
bioRxiv - Immunology 2023Quote: ... anti-human IFN-γ clone 4S.B3) followed by labelling with 1:1000 diluted ExtrAvidin-Peroxidase (Sigma E2886) and developed by with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific™).
-
bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
-
bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti-HISTONE H3 (phospho S10 ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
-
bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
-
bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
-
bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
-
bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
-
bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
-
bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
-
bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
-
bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
-
bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
-
bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
-
bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
-
bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
-
bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
-
bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
-
bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
-
bioRxiv - Cancer Biology 2021Quote: ... after which slides were washed in phosphate-buffered saline and stained against Human Nuclei (MAB4190, 1:250, Millipore) and Human specific Ki67 (MAB4383 ...
-
bioRxiv - Pathology 2020Quote: ... and immunostained with human monoclonal anti-CRP antibody (C1688; Sigma-Aldrich, Saint Louis, MO, USA; 1:400 dilution) which specifically detect the 24-kD monomeric CRP epitope[31 ...
-
bioRxiv - Biochemistry 2020Quote: DNA sequences containing human SUN2 were cloned into a modified bicistronic ampicillin resistant pETDuet-1 vector (EMD Millipore), superfolder GFP-KASH fusions (sfGFP ...
-
bioRxiv - Microbiology 2021Quote: ... HMEC-1 cells (human microvascular endothelial cells RRID:CVCL_0307) were grown in HMEC media (MCDB 131 media (Sigma, M8537) supplemented with 10% FBS (HyClone ...
-
bioRxiv - Immunology 2022Quote: Human THP-1 monocytes were differentiated into macrophages by 24 hr incubation with 100 nM PMA (Sigma-Aldrich) in complete RPMI medium at 1.6 × 105 cells/well in 12-well plates ...
-
bioRxiv - Immunology 2022Quote: ... Human Umbilical Vein Endothelial Cells (HUVEC) were grown in 1% gelatin coated wells in M199 medium (Sigma-Aldrich) containing 20% fetal bovine serum (FBS) ...
-
bioRxiv - Biophysics 2021Quote: ... Samples were stained using a 1:100 dilution of the primary antibodies (rabbit polyclonal anti-human IRSp53, Sigma and rabbit polyclonal anti-human IRTKS antibody ...