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Citations for Millipore Sigma :
701 - 750 of 2420 citations for CEP57 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and preincubated with blocking buffer containing 10% normal donkey serum (D9663, Sigma Millipore), 3% BSA (A2153 ...
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bioRxiv - Developmental Biology 2023Quote: ... and subsequently incubated with blocking buffer (BB) containing 2% BSA (A2153 Sigma Aldrich) and 0.01% Triton-X100 in PBS for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies (see details below) were diluted in blocking solution (5 % BSA (Sigma #A7030 ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking buffer consisted of 5% donkey serum (Millipore Sigma, catalog # S30-100ML) in 0.1% PBST ...
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bioRxiv - Neuroscience 2023Quote: ... Permeabilization and blocking were performed with 1% Triton-X100 (Sigma, Cat. No. T8787), 0.2% gelatin (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... After 1 h in blocking solution (5% milk in TBS-T, Sigma-Aldrich), membranes were incubated in primary antibody solution (1% milk in TBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking solution (10% v/v goat serum/PBS; Sigma Aldrich, St. Louis, MO) was added for 30 min and antibodies were diluted in PBS/0.1% v/v Tween20/5% v/v goat serum (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... samples were then transferred directly into blocking buffer (4 % BSA (Sigma Cat# 10735086001) plus 3 % normal goat serum (Jackson Immunoresearch #005000121 ...
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bioRxiv - Cancer Biology 2024Quote: ... and blocking with 5% (w/v) milk powder in TBST (Tween20-Sigma, #P1379) for 60 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked in blocking buffer (PBS with 2% bovine serum albumin (Sigma) and 0,1% saponin) ...
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bioRxiv - Developmental Biology 2024Quote: ... The blocking solution is exchanged with anti-Digoxigenin-AP Fab fragments (Sigma, 11093274910) diluted 1:2000 in 1% w/v lamb serum in TBST and incubated overnight at 4°C.
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bioRxiv - Developmental Biology 2024Quote: ... blocking was performed in 3% (vol/vol) donkey serum (Sigma-Aldrich, cat.# D9663) or 3% (vol/vol ...
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bioRxiv - Cancer Biology 2024Quote: ... After blocking nonspecific binding sites for 1 h using 5% dry milk (Sigma) in Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were eluted with 40 µl 3x FLAG peptide (Sigma-Aldrich) and collected by centrifugation for 30 min at 100xg.
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bioRxiv - Cell Biology 2020Quote: ... The resulting peptides were desalted with C18 ZipTips (Catalog No. ZTC18S008, Millipore) before LC-MS/MS analysis.
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bioRxiv - Cell Biology 2020Quote: ... ABHD11-FLAG was eluted using 100 mg/l 3xFLAG peptide (Sigma F4799), filtered using a 0.22 μm PVDF filter and separated using a Superdex 75 10/300 column on an Äkta-Pure liquid chromatography system ...
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bioRxiv - Genetics 2021Quote: ... 1:250 rabbit anti-calcitonin gene-related peptide (CGRP) (Millipore PC205L-100UL), 1:250 rat anti-myelin basin protein (MBP ...
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bioRxiv - Developmental Biology 2020Quote: ... The resultant peptides were desalted with Zip Tip pipette tips (Merck Millipore). The tandem mass spectrometry spectra were searched against the C ...
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bioRxiv - Developmental Biology 2020Quote: ... dried peptides were resuspended in 2.5 % 1,1,1,3,3,3-Hexafluoro-2-propanol (Sigma-Aldrich) / 0.1 % TFA prior to LC-MS measurement ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by elution with 100 μg/ml c-Myc peptide (Sigma-Aldrich) on an end-to-end rocker at room temperature for 1 hr ...
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bioRxiv - Molecular Biology 2021Quote: Digested peptides were desalted using in-house reverse-phase C18 chromatography (Millipore) in preparation for mass spectrometry (MS ...
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bioRxiv - Microbiology 2019Quote: ... The eluted peptides were desalted with C18 Millipore® ZipTip columns (Millipore) according to the manufacturer’s guidelines.
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bioRxiv - Genetics 2021Quote: ... the wash buffer was supplemented with 0.1mg/mL 3X-FLAG peptide (Sigma). Elutions were then loaded onto a HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Genetics 2020Quote: ... and proteins were eluted using 100 µg/mL 3x FLAG peptide (Sigma). Eluates were analysed by western blotting with anti-FLAG antibodies (1:5000 ...
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bioRxiv - Genetics 2020Quote: ... Beads were washed and eluted using 100 µg/mL 3xFLAG peptide (Sigma). For detection ...
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bioRxiv - Immunology 2021Quote: ... the peptides were dried and extracted using C18 Zip-tip (Merck Millipore).
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bioRxiv - Biochemistry 2021Quote: ... peptides were desalted using Millipore U-C18 ZipTip Pipette Tips (Millipore-Sigma). A 2cm long x 75μm ID C18 trap column was followed by a 25cm long x 75μm analytical columns packed with C18 3μm material (Thermo Acclaim PepMap 100 ...
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bioRxiv - Biochemistry 2021Quote: ... peptides were desalted using Millipore U-C18 ZipTip Pipette Tips (Millipore-Sigma). A 2cm long x 75μm ID C18 trap column was followed by a 25cm long x 75μm analytical columns packed with C18 3μm material (Thermo Acclaim PepMap 100 ...
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bioRxiv - Biochemistry 2020Quote: ... the peptide preparations were desalted using Zip-tip C18 cartridges (Millipore, MA) and reconstituted with 0.1% formic acid (FA).
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bioRxiv - Cancer Biology 2021Quote: ... The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore) and analyzed either in technical triplicates or individually by a Q-Exactive Plus coupled to a Nano-LC Proxeon 1000 equipped with an easy spray ion source (all from Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... and protein was eluted with 100 µg/mL FLAG peptide (Sigma-Aldrich) in elution buffer for 1 hour at 4°C with rotation ...
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bioRxiv - Plant Biology 2020Quote: ... Beads were washed before incubation with 3XFLAG® Peptide (F4799, Sigma-Aldrich) for 2 hrs ...
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bioRxiv - Plant Biology 2020Quote: ... and peptides from were purified using Zip tip C18 pipette tips (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 50 μL of Elution Buffer (150 μg/mL 3xFlag peptide [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2021Quote: ... Other reagents for peptide synthesis and solvents were purchased from Sigma-Aldrich.
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bioRxiv - Neuroscience 2020Quote: ... the peptides were resuspended in dry dimethylsulfoxide (DMSO; 5 mM, Sigma Aldrich) and Hams F-12 (PromoCell Labclinics ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... and an RGD peptide (Gly-Arg-Gly-Asp-Ser/GRGDS, Sigma-Aldrich) for 20 min at room temperature before the cells were added to the wells for incubation for 3 hours at 37°C.
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bioRxiv - Cell Biology 2021Quote: ... was added 2 h after peptide addition while monensin (Sigma-Aldrich #475895) was added 6 h later at a concentration of 0.75 μM ...
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bioRxiv - Plant Biology 2022Quote: ... using the synthetic peptide substrate Z-Phe–Arg-methylcoumarin (MCA) (Sigma-Aldrich). Hydrolysis was allowed to proceed at 25°C in reducing conditions (10 mM L-cysteine ...
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bioRxiv - Biochemistry 2022Quote: ... and associated proteins were eluted in 125 μg/ml 3xFLAG peptide (Sigma) for 30 minutes on ice.
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bioRxiv - Microbiology 2022Quote: Digested peptides were dissolved in 0.1% formic acid (Sigma-Aldrich, cat#06440) containing independent retention time (iRT ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitants were eluted with 100 mM 3X FLAG peptide (Millipore Sigma, F4799) in lysis buffer rotating for 1 hour at 4°C and processed for immunoblotting.
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bioRxiv - Cell Biology 2022Quote: ... Puromycylated peptides were immunoprecipitated using the 12D10 anti-puromycin antibody (MABE343; Millipore) bound to protein A agarose beads (11719408001 ...
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bioRxiv - Plant Biology 2021Quote: ... The digested peptides were desalted using micro ZipTip mini-reverse phase (Millipore), and then lyophilized to dryness ...
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bioRxiv - Immunology 2021Quote: Peptides with predicted binding to DR0401 (Supplemental Table 3) were synthesized (Sigma) and their binding to DR0401 was assessed through a competition assay ...
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bioRxiv - Neuroscience 2021Quote: ... and protein was eluted with 100 μg/mL FLAG peptide (Sigma-Aldrich) in elution buffer for 1 hour at 4°C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... Tryptic digested peptides were then desalted and purified using a Ziptip (Millipore) as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the beads were incubated with 0.2 mg/mL single FLAG-peptide(Sigma) in TAP buffer and rotated at 4 °C for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... (6) mouse anti-calcitonin gene-related peptide (CGRP; 1:100, Sigma, USA) of neuronal peptide in the nervous tissues ...