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Citations for Millipore Sigma :
701 - 750 of 8389 citations for 9 3 bromophenyl 9 phenyl 9H fluorene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Female C57BL/6 mice wild type mice (7-9 weeks) were injected intraperitoneally with etomoxir (100 µg, Sigma Aldrich, Cat. No E1905) and/or LPS (1 µg ...
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bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30%, Sigma-Aldrich, Germany) or A was 0.1% formic acid (for celesticetin detection ...
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bioRxiv - Biophysics 2021Quote: ... 800 µL of culture was layered over 700 µL cold silicone oil (9:1 AR20/Sigma High Temperature, cooled to -78°C) and cells pelleted at 13,000 r.c.f ...
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bioRxiv - Synthetic Biology 2022Quote: ... a calibration curve of resorufin concentrations versus RFU was plotted using solutions prepared with a standard compound (Sigma-Aldrich, 635-78-9) (Extended Data Fig ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... HT29-MTX cells were either grown post confluent for 9 days as described previously (30) or stimulated with 1.25 mM butyrate (Merck Millipore, Burlington, Massachusetts, US) for 72 h in the presence or absence of 1 µg/ml LPS-EB ultrapure (InvivoGen ...
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bioRxiv - Cell Biology 2020Quote: ... Larvae were then fixed in 1 ml Bouin’s solution (5% acetic acid, 9% formaldehyde, 0.9% picric acid; Sigma Aldrich, St. Lous, MO) for 2 hrs at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... generally between 8 and 10 mg of nuclear extract (approximately 1.9 mL of extract depending on the concentration) was mixed with 100 μL anti-Flag M2 affinity agarose slurry (Sigma-Aldrich, #A2220) washed with 0.1 M glycine then equilibrated in binding buffer (20 mM HEPES pH7.4 ...
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bioRxiv - Genetics 2021Quote: Recombination of the Bbs5flox/flox allele was induced in juvenile CAGG-creERT2 (Jax Mice stock# 004682) positive mice at postnatal day 7 by a single intraperitoneal (IP) injection of 9 mg tamoxifen (Millipore Sigma, T5648) per 40 g body weight ...
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bioRxiv - Developmental Biology 2022Quote: ... WT/IWR consists of 9 wild type embryos and 8 embryos treated with the small molecule inhibitor 10µM IWR1 (Sigma Aldrich, Cat I0161). DMSO/DMH4 includes 12 embryos at 3 dpf ...
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bioRxiv - Microbiology 2024Quote: ... Long-term freezer stocks of collected parasites were prepared by mixing 100 uL of collected blood with 400 uL Freezing Solution (9:1 Alsever’s solution (Sigma-Aldrich, Cat#A3551):glycerol) ...
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bioRxiv - Physiology 2022Quote: ... Protein contents of mitochondrial pellets were extracted by adding 4X volume of 1x MAS buffer followed by 1/9 volume of 10% n-dodecyl β-D-maltoside (DDM; Sigma D4641) for a final 1% DDM concentration ...
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bioRxiv - Bioengineering 2024Quote: ... gelatin was dissolved in 10 w/v% sodium carbonate– bicarbonate (CB) buffer (0.25 M, from Sigma Aldrich, pH was adjusted to 9) at 50 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Concentration–response curves were obtained in responses to increasing concentrations of acetylcholine (Ach, 10−9 to 10−5☐M; Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... An incision is made between the thoracic and lumbar vertebrae to access the dorsal funiculus of the spine and an intraspinal injection of 1 μL of lysophosphatidylcholin diluted into NaCl 9‰ (LPC, 10mg/mL; Sigma-Aldrich L4129) is made with a glass capillary ...
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bioRxiv - Systems Biology 2023Quote: Caspase-9 activity was measured in liver lysate according to manufacturer’s protocol (Merck Caspase-9 Colorimetric Activity Assay Kit APT139, EMD Millipore Corporation, USA). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... and again treated with 20μMZ-VAD-fmk freshly added 1h before adding Gal-9 or Staurosporin (Sigma Aldrich, St. Louis, MO, USA). For long-term assays using patient-derived AML cells (5-7 days) ...
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bioRxiv - Cell Biology 2023Quote: ... 9 g l-1 Nembutal, Sigma-Aldrich Cat# P37610; 20.8 g l-1 MgSO4, Sigma-Aldrich Cat# 63138; 10% ethanol, Sigma-Aldrich Cat# 459836 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were transfected with constructs expressing pAAV-hSyn-Nb-FT-2-TRPV1Ca2+ or pCMV-TRPV1Ca2+ 24 h after plating using X-tremeGENE™ 9 DNA Transfection Reagent (Millipore Sigma) according to manufacturer’s specifications ...
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bioRxiv - Biochemistry 2024Quote: ... along with packaging vectors psPAX2 (#12260) and PMD2.G (#12259) were transfected into the HEK293-FT cells using XTremeGene 9 (Sigma Aldrich, 6365779001) (A375m ...
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bioRxiv - Microbiology 2024Quote: ... Three clams were collected in the same stomacher bag and homogenised together by adding 9 volumes of phosphate buffered saline (PBS) (Sigma-Aldrich, Germany) of the sample weight for 2 minutes at maximum speed using the Stomacher® 3500 (VWR ...
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bioRxiv - Biophysics 2024Quote: ... γ-cyclodextrin (γ-CD, CAS#7585-39-9), C-reactive protein (CRP, AG723) and human serum (H4522-100ML) were ordered from Sigma-Aldrich. 1 µM γ-CD stock suspension was prepared with MilliQ ...
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bioRxiv - Cancer Biology 2024Quote: ... cast from ultra-pure agarose (20 mg/mL in Milli- Q water with 9 mg/mL NaCl) using a 3D micro-mold (3D Petri Dish®, Sigma Aldrich). Once solidified ...
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bioRxiv - Cell Biology 2024Quote: iBMDM and RAW cells were transfected with custom CRISPR gRNA plasmid DNA (U6-gRNA:CMV-Cas-9–2 A-tGFP; Sigma-Aldrich, Oakville, Canada) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with doxycycline (1 µg/ml), Compound E (0.1 µM, Enzo Life ALX-270-415-C250) and FuDR (8 μM, Sigma 50-91-9). From then on ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.003% 1-phenyl-2-thiourea (PTU) (Sigma Aldrich, P7629) was added to 0.3X Danieau’s buffer at 8 hours post-fertilization in order to prevent pigment formation ...
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bioRxiv - Bioengineering 2022Quote: ... and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, ≥95%, Sigma-Aldrich), 4-arms thiolated PEG (PEG4SH ...
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bioRxiv - Plant Biology 2020Quote: ... pentaerythritol and phenyl b-D-glucopyranoside (both Sigma-Aldrich) were spiked to the extracts before drying ...
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bioRxiv - Immunology 2020Quote: ... plus 0.003% 1-phenyl-2-thiourea (P7629 Sigma-Aldrich).
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bioRxiv - Developmental Biology 2021Quote: ... containing 0.003% 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) to avoid pigmentation and Tricaine at 0.08 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... 0.003% (v/v) 1-phenyl-2-thiourea (PTU) (Sigma) was added after 10 - 12 h[42] ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.2 mM of 1-phenyl-2-thiourea (PTU, Sigma) was added to the E3 media from 24 hpf onwards.
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bioRxiv - Bioengineering 2024Quote: ... with Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, Sigma-Aldrich, 900889) at a concentration of 0.16 mg mL−1 and filter-sterilized at 40°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 1-Phenyl-2-thiourea (PTU, 75 mM, Sigma, #P7629) was added to inhibit melanogenesis (Karlsson et al. ...
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bioRxiv - Bioengineering 2023Quote: ... The photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP; Sigma Aldrich) was added for a final concentration of 2.2 mM ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 1-Phenyl-2-thiourea (PTU, Sigma-Aldrich) after 24 h post fertilization (hpf) ...
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bioRxiv - Bioengineering 2024Quote: ... LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate, Sigma Aldrich GmbH, Germany), Kanamycin (CAS No ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then cleared by replacing ethanol with ethyl cinnamate (ECi; Ethyl 3-phenyl-2-propenoate; Sigma-Aldrich, order no. 112372-100G). After 30-60 min ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: Cell culture supernatant was diluted 1:1 in binding buffer (0.16 M sodium phosphate, 500 mM NaCl, pH 9) and loaded on a Protein A Sepharose (Sigma Aldrich, 17-1279-03) column at a flow rate of 2 ml/min ...
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bioRxiv - Physiology 2020Quote: ... The other half of the conditioned media was subjected to IgG pull-down step (2 µl for 9 ml CM) (Anti-mouse IgG monoclonal Ab; Sigma-Aldrich; Cat#M9144) which was used as a negative control for this assay ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Microbiology 2022Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... and 78.1 µL of a 10 mg/mL (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate (BCN, 17 eq.) (Sigma Aldrich, Cat. No. 744867-10MG) and stirred at 4°C for 16 h ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with a pDisplay vector encoding amino acids 16 to 682 of SARS-CoV-2 S (S1) fused to a C-terminal hemagglutinin (HA) peptide using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... chacei subadults were progressively dehydrated in a PBS-ethanol series (ambient temperature) before embedding in polyethylene glycol distearate-1-hexadecanol (9: 1) resin (Sigma, St. Louis, MO). Resin blocks were stored in −20°C until trimming into 10 µm sections with a RM 2255 microtome (Leica Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 5 µg of EPS15 or DECR1 expression plasmids using X-tremeGENE™ 9 DNA Transfection Reagent (Sigma-Aldrich, 6365787001) using 1:3 DNA:reagent ratio ...
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bioRxiv - Neuroscience 2024Quote: ... pups PND 8-9 were given an intraperitoneal (i.p.) injection of lipopolysaccharide (LPS, 40 μg/kg; E. coli O111:B4, Sigma; dissolved in sterile saline) or vehicle (saline) ...
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bioRxiv - Cell Biology 2023Quote: Cells were arrested in prometaphase by incubating them for 2 – 4 h in 200 ng ml-1 nocodazole (Sigma-Aldrich, 31430-18-9). Mitotic exit was induced by adding flavopiridol (Tocris Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... a human high grade urothelial cancer (obtained from ATCC, HTB-9™) and MB49 cells (murine urothelial carcinoma cells, 7,12-dimethylbenz[a]anthracene, DMBA, EMD Millipore, Cat. SSC148) were maintained as monolayers in RPMI1640 medium supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: Tissue sections were prepared by embedding dissected organs (foregut, midgut tube and scaphognathites) in polyethylene glycol distearate-1-hexadecanol (9 : 1) resin (Sigma, St., Louis, MO) after progressive dehydration and soaking into the resin (PBS – ethanol series at ambient temperature and ethanol – resin series at 40°C ...