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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for 7 NITRO 2 1H QUINOXALINONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Brain sections were first incubated for 1h at room temperature (RT) in a blocking solution containing 1% BSA (Sigma), 2% Donkey Serum (Biowest ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Neuroscience 2021Quote: ... washed 3 times for 5 min with PBS and permeabilized for 1h at room temperature (permeabilization buffer: 2,5% donkey serum (Sigma), 1% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were then incubated 1h at RT with the rabbit polyclonal antiTWIST1 (1/50, ABD29, Millipore, Burlington; MA, USA); or 1h at 37°C with the sheep polyclonal anti-TWIST2 (1/200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole cell extracts (500 μg per IP) were immunoprecipitated for 1h using a PHGDH-specific antibody (Merck Sigma, HPA021241) complexed with Dynabeads Protein A (Life Technologies) ...
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bioRxiv - Physiology 2022Quote: ... and kept at 4°C for 1h in KB solution containing 50 mg/ml polyvinylpyrrolidone (PVP, Sigma, Chemical Co.). Finally ...
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The lysates were then precleared for 1h at 4°C using 10µl protein A agarose 50% slurry/plate (Millipore), and collected by centrifugation at 300g for 3min at 4°C (100µl of sample/plate was kept as input chromatin) ...
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bioRxiv - Physiology 2020Quote: ... Slides were then washed twice in warm water for 5 min and stained for 1h at RT with Picro-Sirius Red solution (0.1% (m/v) Sirius Red (Sigma) in saturated aqueous solution of Picric Acid (LabChem)) ...
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bioRxiv - Developmental Biology 2021Quote: ... plates were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Sigma-Aldrich, 5µg/ml in PBS, 1h at RT).
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bioRxiv - Immunology 2022Quote: ... was applied for 1h prior to staining with the indicated primary antibody diluted in PBS solution with 0.5% Goat Serum (Sigma), 0.5% BSA (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 1H NMR was acquired with 64 scans on a Jeol 400 MHz spectrometer using deuterated water (D2O; Sigma-Aldrich) as an internal standard ...
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bioRxiv - Cancer Biology 2022Quote: ... the zebrafish larvae were permeabilized for 1h at RT with a blocking buffer [5% BSA (A9647-10G, Sigma-Aldrich), 5% sheep serum (013-000-1210 ...
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bioRxiv - Immunology 2023Quote: ... the reaction mixture was incubated at 37°C for 1h and its aconitase activity was determined per manufacturer’s instructions (Aconitase Assay Kit from Sigma).
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bioRxiv - Developmental Biology 2023Quote: ... for 1 min 30 seconds at high intensity in a 200 mTorr vacuum and then incubated at room temperature for 1h with 200µL/well of 200 µg/mL Poly-D-Lysine (A-003-E, Merck Millipore) diluted in 0.1M HEPES pH 8.4 (H3784-25G ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked with 5% normal donkey serum for 1h at room temperature and incubated with Nissl (1:2000, in 0.1M PB, Sigma) for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... sample could be incubated also for 1h with oligo-dT-dig probe (1:1000, polyT-dig Sigma HA09131354-004). Antibodies were diluted 1:500 in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... the blot was again washed with block buffer and incubated for 1h with either Monoclonal ANTI-FLAG M2 (Sigma) (1:7500 dilution ...
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with the following primary antibodies for 1h: mouse anti-α-Tubulin (1:1000, T5168, Sigma-Aldrich) and rabbit anti-Lamin A (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: Coverslips with either adult mouse or neonatal rat cardiomyocytes were incubated 1h with anti-α-actinin antibody (A7811, Sigma) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed samples were washed rapidly twice with PBS and 3 times for 15 min with PBS-0.3% Triton X-100 (PBT) before being pre-incubated for 1h in PBT-1% bovine serum Albumin (BSA, Sigma). Samples were incubated overnight at 4°C with primary antibody diluted in PBT-1% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were permeabilized for 1h at room temperature in 0.1 M PBS containing 0.5% Triton X-100 (Sigma Aldrich), followed by 1.5 h incubation in blocking solution of 0.1 M PBS containing 0.3% Triton X-100 and 5% Normal Donkey Serum (NDS ...
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bioRxiv - Neuroscience 2022Quote: ... were dissected as mentioned above and fixed with 2% paraformaldehyde and 0.06% glutaraldehyde in PEM (0.1 M PIPES: P1851-500g, Sigma, Germany; 2 mM EGTA: E3889, Sigma, Germany; 1 mM MgSO4 x 7 H2O, v/v) for 90 min on ice ...
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bioRxiv - Genetics 2021Quote: ... followed by a 1h 15min incubation at 45 °C with 10 μl of Proteinase K (10mg/ml Millipore, cat#70663). One volume of phenol/chloroform was then added to each lysate ...
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bioRxiv - Developmental Biology 2020Quote: ... at 56°C for 40min and re-probed with anti– beta-actin antibody for 1h (1:1000, Sigma, ref. A5441).
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bioRxiv - Neuroscience 2021Quote: ... maintained for at least 1h at 4°C and then transferred onto semiporous membranes inserts (47 mm diameter, 0.45 µm pore size; Millipore FHLP04700) which were placed in six well tissue culture plates containing 1.1 ml medium per well ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was split in half and incubated for 1h at RT (while rotating) with either anti-FLAG M2 affinity gel (Sigma) or anti-HA affinity matrix (mAb 3F10 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with primary antibodies in BB for 1h at RT: monoclonal mouse anti-Col4a2 (Millipore, Cat. No. MAB1910), polyclonal rabbit anti-LM (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... blots were subsequently incubated for 1h at room temperature with horseradish peroxidase-conjugated secondary anti-rabbit/mouse antibodies (1:10000; Sigma). Immunoreactive bands were visualized by chemiluminescence detection of peroxidase activity (SuperSignal West Dura ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
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bioRxiv - Neuroscience 2021Quote: ... resuspended in PBS for 15 minutes and then incubated 1h at 37°C with 60 µg/ml of PI (Sigma). Cells were collected by centrifuge and resuspended in ice-cold PBS for FACS analysis.
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bioRxiv - Biochemistry 2020Quote: The formation of homoserine lactone in the enzymatic reaction was confirmed by comparison with the 1H NMR spectrum of an authentic sample (from Sigma) dissolved in the same buffer ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed multiple times in TBS-T for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; Sigma) as above at 1:2500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... bicuculline (20 μM, acute(0-1h) or 48 h), picrotoxin (10 μM, 1 h), CNQX (10 μM, 1 h) (Sigma), and synthetic Aβ1-42 (Tocris ...
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bioRxiv - Microbiology 2022Quote: ... Nitrocellulose membrane was washed in TBST for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; catalogue number A3562) (Sigma,) in 5% skim milk TBST at 1:2500 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1h at 37°C and then with 20μL 1% tert-butyldimethylchlorosilane in N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide (Sigma Aldrich) for 3h at 60°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Germany) slides were incubated for 1h at room temperature (RT) in TBS containing 10% donkey serum (Sigma-Aldrich, Vienna, Austria), 1% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... were blocked for 1h in TBS containing 0.1% Tween-20 (TBS-T) supplemented by 3% fatty-acid free BSA (Sigma Aldrich) before incubation with the purified STARD10 protein (1 μg/mL in TBS-T + 3% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... and or Alexa Flour 488 anti-mouse IgG (1:800, A11001, Life) at room temperature for 1h and mounted with DAPI (F6057, Sigma). The sections were observed using fluorescence microscope (Olympus BX35 ...
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bioRxiv - Pathology 2021Quote: ... Fixed cells were then washed with 1X PBS and stained for 1h at room temperature with 0.1% crystal violet (Sigma-Aldrich) prepared in 10% ethanol/water ...
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bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... Molten paraffin was then changed five times (each change lasting >1h) before the tissue was transferred into a Peel-A-Way embedding mould (Sigma) for transverse sectioning ...
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bioRxiv - Neuroscience 2023Quote: ... Permeabilisation was achieved by incubation for 1h in freshly prepared blocking solution (PBS with 5% (v/v) normal donkey serum (D9663, Sigma) and 0.3% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were blocked with 3% BSA in PBS with 0.3% Triton X-100 for 1h at room temperature and subsequently incubated with primary antibodies (rabbit anti-glutamate, 1:500, Sigma; rabbit anti-SOM ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS to permeabilise and a second time for 1h in 0.5% Triton X-100 1% fish gelatin (CAS #9000-70-8, Sigma-Aldrich) in PBS to block ...
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bioRxiv - Cell Biology 2024Quote: ... Bound material was eluted (1h at RT, shaking at 850 rpm) from anti-FLAG M2 magnetic beads (Sigma, Cat. # M8823) with homemade 10 µg/µL Strep-6xFLAG-peptide in PBS supplemented with 0.1% glyco-diosgenin ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were extracted from untreated (0) and UV-C treated WT and jmj27 plants (time points 30 min, 1h and 2h) using Tri-Reagent (Sigma). Reverse transcription reaction (RT ...
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bioRxiv - Molecular Biology 2024Quote: ... DIvA cells were pretreated for 1h and during subsequent DSB induction with either 20µM ATMi (KU-55933 from Sigma, SML1109), 2µM ATRi (ETP-46464 from Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... Magenta GA-7 vessels (Sigma-Aldrich, St. Louis, MO, USA), allowed to air dry for 25 min in the hood prior to vessel closure ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained with 7-aminoactinomyocin D (7AAD; Sigma Life Science) to exclude dead cells ...