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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for 7 Hydroxy 2 3 dihydro 1H cyclopenta c chromen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were also washed for 10 minutes in a solution of 1.25×10−3 mg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Sigma: D9542) in PBS to fluorescently label nuclei within tissue sections.
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... tricaine-anesthetized 3 or 4 days-post fertilization (dpf) larvae were mounted in 2% low-melting point agarose (Sigma-Aldrich) on a 35 mm glass bottom dish (#1.5H Glass ...
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bioRxiv - Immunology 2023Quote: ... 3-4 and 7-9 were treated with 100 µL of embryo water plus 1 µg/mL 2′,7′-Dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) in 0.4% DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... sodium-3-methyl-2-oxobutyrate (ketovaline, Sigma), 3-methyl-2-oxovaleric acid sodium salt (ketoisoleucine ...
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bioRxiv - Developmental Biology 2020Quote: ... phosphatase inhibitor cocktail 2 and 3 (Sigma), and cOmplete EDTA-free protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2-3 mL mineral oil (M8410, Sigma) was used to fully overlay the droplets to prevent the evaporation.
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bioRxiv - Neuroscience 2020Quote: ... 3 mM Mg(Ace)2 (Sigma #63052), 10mM Tris-HCl (pH 8 ...
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bioRxiv - Neuroscience 2020Quote: ... 3 mM Mg(Ace)2 (Sigma #63052), 10mM Tris-HCl (pH 8 ...
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bioRxiv - Neuroscience 2020Quote: ... 3 mM Mg(Ace)2 (Sigma #63052), 10mM Tris-HCl (pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... indole-3-actic acid (Sigma, 2 mM) were freshly prepared for serial dilutions (10x ...
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bioRxiv - Neuroscience 2022Quote: ... phosphatase inhibitor cocktails 2 and 3 (Sigma). Lysates sat on ice for 30min with intermittent vortexing ...
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bioRxiv - Bioengineering 2021Quote: ... + 0.05 × 10−3 M 2-mercaptoethanol (Sigma)] supplemented with soluble anti-mouse CD28 (5 µg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... 3-methylene-2-norbornanone (Sigma; Cat# M46055), lumiflavin (Cayman Chemical ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2-ethyl-3-methylpyrazine (Sigma W315508), 1% 2,5-dimethylpyrazine (Sigma 17542015) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1% phosphatase inhibitor cocktail 2 & 3 (Sigma) and protease inhibitor cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... 1x phosphatase inhibitor cocktail 2 and 3 from Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... phosphatase inhibitor cocktail 2 and 3 (Sigma) and benzonase (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... and phosphatase inhibitors 2 and 3 (Sigma). Lysates incubated on ice for at least 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... β2/3 subunit (Millipore, catalog #: 05-474) (1:250 dilution) ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 10 min 100% EtOH) and cleared (3 x 5 min) in xylene (247642, CAS: 1330-20-7, Sigma Aldrich). Finally ...
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bioRxiv - Plant Biology 2023Quote: ... 2 mM Ca(NO3)2 ×24H2O (13477-34-4, Sigma), 2.5 mM KH2PO4 (7778-77-0 ...
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bioRxiv - Immunology 2022Quote: ... or MDM (106) were treated for 1h at 37°C with 1 ml of VLPs-VPX+ containing 5 μg/ml polybrene (Sigma, #H9268) for SAMHD1 degradation16,37 before the addition of 20 µg CAp24 of each control or/and encoding-gene lentiviral vectors with 5 μg/ml polybrene for 2h transduction ...
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bioRxiv - Developmental Biology 2019Quote: Somite extract (200μg protein for each condition) was incubated at 37°C for 1h in the presence of 300μM D-NAME (Sigma, Lot#BCBM7105V) or L-NAME (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with primary antibodies in blocking buffer for 1h at 21°C: 1:1000 α-tubulin (118M4779V; Sigma Aldrich), 1:250 CREST (15-234-0001 ...
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bioRxiv - Cancer Biology 2023Quote: ... the tissue fragments were washed with ice cold phosphate-buffered saline (PBS) and were subsequently enzymatically digested at 37°C for 1h using 5 mg/mL collagenase II (Sigma-Aldrich), 10 μM Y-27632 (Cayman Chemicals ...
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bioRxiv - Neuroscience 2024Quote: ... for one hour before overnight incubation at 4℃ with one of the following primary antibodies: anti-NG2 (1:200; Millipore AB5320), anti-Laminin (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... 4% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate (CHAPS; Sigma-Aldrich, Cat. No. 226947)] ...
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bioRxiv - Cell Biology 2022Quote: ... Primary human lung fibroblasts of passage 5 until 7 (table 2) or CCL206 fibroblasts were proliferation-inactivated with mitomycin C (10 μg/ml, Sigma-Aldrich, St. Louis, MO, USA, #M4287) for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing PelXC232S were pooled and concentrated to a volume of 2 mL by centrifugation at 2 200 × g at a temperature of 4 °C using an Amicon ultra centrifugal filter device (Millipore) with a 10 kDa molecular weight cut-off ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Biophysics 2020Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Virus containing fractions were collected ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Biophysics 2021Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Part of the supernatant containing pelleted viruses was collected ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were equilibrated at 4 °C and incubated for 30 min in the presence of 2 mg/ml iodoacetamide (Sigma). Membranes were then solubilized in 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... stratified for 2 d at 4°C in the dark and sown on Gamborg’s B5 Basal Salt medium (Sigma G5768) supplemented with 1% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... at 25,000 x g for 2 h at 4°C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were discarded ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Pathology 2021Quote: ... at 25,000 x g for 2 hours at 4 °C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were decanted ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...