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Citations for Millipore Sigma :
7401 - 7450 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... To create the Tmem98A193P allele Edit-R crRNA (sequence 5’-CCAAUCACUGUCUGCCGCUG-3’) (Dharmacon) was annealed to tracrRNA (Sigma) in IDT Duplex buffer (IDT) ...
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bioRxiv - Bioengineering 2019Quote: ... Chips fabricated with polyester terephthalate (PET) track-etched membranes were assembled using (3-Glycidyloxypropyl) trimethoxysilane (GPTMS; Sigma, 440167) and (3-Aminopropyl ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 x in ice-cold choline-buffer and lysed in 0.5% Triton X-100 (Sigma) in double-distilled water for 20 min at room temperature with gentle agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted nsp7 sample was concentrated with a 3 kDa molecular weight cut-off Centrifuge Filter (Millipore Corporation) and then size-separated by a Superdex S75 10/300 GL column in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were split into 3 replicates containing 6.6 million cells each and treated with either DMSO (Sigma-Aldrich) or 40nM erlotinib (Selleckchem) ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were then stained at room temperature in 0.05 M Tris-HCl pH 7.6 buffer containing 0.5 mg/ml 3-39 diaminobenzidine (DAB, Sigma Aldrich) and 0.01% hydrogen peroxide.
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed with 1X TBS 3 × 5 minutes followed by treatment with 0.05% 3,3’-Diaminobenzidine (Sigma Aldrich) and 0.01% H2O2/1X TBS for 20 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... The plates were washed 3 times with pre-cooled PBS prior to the addition of L-DOPA (Sigma). After incubation in L-DOPA for 1 day ...
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bioRxiv - Biochemistry 2019Quote: ... Isolated protein was then buffer exchanged with 0.5 M Triethyl ammonium bicarbonate (pH 8.5) using a 3 kDa cutoff centrifugal filter (Amicon, Millipore). Protein was then estimated using Bradford’s reagent (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... Dialyzed CTPS2 was then concentration approximately 5-fold using a 3 kDa cut-off centrifugal filter unit (Millipore), flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... cells at 3–4 DIV were incubated with culture medium containg 0–0.8 mM methylmethanesulfonate (MMS, Sigma, 129925) or 0–8 µM etoposide (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... Fixed collagen samples were washed 3 times with a solution containing 50 mM PIPES pH 7.4 (Sigma, P6757) kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI ...
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bioRxiv - Plant Biology 2020Quote: ... low-fat dry milk) or anti-GFP (Sigma-Aldrich, dilution 1:1000 in TBS-T with 3% BSA) primary antibody at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Mo and PN were then washed in DMEM and placed in 3 μm Boyden chambers (Merck Millipore, France), at the concentration of 0.5 x 106 cells/chamber in 200 µl DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... washed 3 times with phosphate-buffered saline (PBS) and permeabilized in 0.4% Triton X-100 (T878, Sigma-Aldrich), 1% donkey serum (D9663 ...
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bioRxiv - Physiology 2020Quote: ... Each experimental condition was plated in triplicate and 3 wells were always treated with fulvestrant (ICI182,240) (Sigma Aldrich) in order to measure the basal ERα transcriptional activity ...
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bioRxiv - Cancer Biology 2019Quote: Cells were treated with blebbistatin (20μM, 3 hr) (Selleckchem, S7099), fasudil (5μM, 24 hr) (Selleckchem, S1573), ML7 (20μM, 1 hr) (Sigma, I2764), ionomycin (10μM ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore). The protein was conserved in 10 mM Tris-HCl pH7.63 and 33% glycerol ...
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Microbiology 2019Quote: ... Membranes were blocked in 0.05% v/v Tween-3% v/v bovine serum albumin-PBS (Sigma Aldrich, USA) overnight at 4°C and washed twice with 0.05% v/v Tween (Sigma Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... permeabilized in 0.1% v/v Triton X-100 in PBS and blocked in 3% w/v BSA (Sigma) in PBS for 1 hr ...
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bioRxiv - Biophysics 2020Quote: ... Cleaned glass coverslips were then treated during one hour with 3-(trimethoxysilyl)propyl acrylate (Sigma #2530-85-0) to promote a strong adhesion between the hydroxy-PAAm hydrogel and the glass coverslips and finally dried under a nitrogen flow ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant (total protein concentration ∼3 mg/ml) was incubated with ANTI-FLAG M2 agarose beads (Sigma Aldrich) at 4°C for 1 h with agitation ...
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bioRxiv - Bioengineering 2021Quote: ... then rinsed 3 times with PBS and permeabilized with staining buffer (0.1% Triton X-100/PBS, Sigma, 93443) for 1 hour before immunolabeling.
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bioRxiv - Plant Biology 2021Quote: ... 250 μM or 1 mM L-glutamic acid monosodium salt monohydrate (Sigma Aldrich, cat No. 6106-04-3). Plants were analyzed in single blinded-experiments ...
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bioRxiv - Cell Biology 2021Quote: ... TACC3 (5’ GAGCGGACCUGUAAAACUA 3’) and a non-targeting oligo against Luciferase was used as control (Sigma, Custom order). RNAi Max (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... 3 mM MgCl2) containing 80 μl NBT (Sigma) (35mg/ml in70% DMSO and 30 μl de BCIP (Sigma) (50 mg/ml in 100% de DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... On the day of the experiment cells were treated with 3 ng/ml IL-1α (SRP3310, Millipore Sigma), 30 ng/ml tumor necrosis factor (8902SF ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were centrifuged again at 1,500 for 3 minutes and pellets were resuspended in 0.25% Triton X-100 (Sigma), transferred to 1.5 ml tubes ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then fixed in 3% paraformaldehyde in PBS for 15 minutes and stained with DAPI (Sigma-Aldrich). Images were taken with Nikon Eclipse TE2000-E inverted fluorescence microscope equipped with a CCD-camera.
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bioRxiv - Bioengineering 2020Quote: ... Cells were rinsed 3 times in 1X PBS and permeabilized with 0.1% Triton X-100/PBS (Sigma, 93443) for 15 min at RT ...
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bioRxiv - Bioengineering 2021Quote: Before staining with either 5-bromo-4-chloro-3′-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, Sigma-Aldrich) for alkaline phosphatase (ALP ...
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bioRxiv - Biochemistry 2021Quote: ... 3 nM of detergent-solubilized or nanodisc-reconstituted complex I and either 1 mM FeCy (Sigma Aldrich BVBA) or 100 μM Q1 (Sigma Aldrich BVBA ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using 3 kDa (KrACP, FabD) and 30 kDa (KrKSCLF) Amicon Ultra (Millipore, 15 mL capacity) molecular weight cutoff (MWCO ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927; 20mMTris-HCL pH 7.6, T7943, Sigma, 150mM NaCl, Sigma) then stained with Ponceau S (PoncS ...
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bioRxiv - Neuroscience 2021Quote: ... both labels were purified using 3 kDa molecular weight cut-off ultrafiltration filter (Amicon Ultra Centrifugal Filter, Millipore). There was no detectable dye in the filtrate ...
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bioRxiv - Neuroscience 2021Quote: ... KO and Floxed female and male mice were intraperitoneally injected with 3 mg/kg LPS (O111:B4, Sigma). 24 h after ...
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bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... The selectivity of the HIS3 reporter gene was eventually modulated with 3-aminotriazole (Sigma, St Louis, MO, USA) in order to obtain a maximum of 285 histidine-positive clones for 50 millions diploids screened ...
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bioRxiv - Cancer Biology 2021Quote: ... Metabolic activity was measured by adding 10 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 10 mg/ml, Sigma). After four hours of incubation ...
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bioRxiv - Biophysics 2020Quote: ... cleaned and silanized with (3-aminopropyl)trimethoxysilane (APTMS; unless otherwise stated, all chemicals from Sigma-Aldrich, Dorset, UK) for 3 min (minutes) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.1% SDS with cOMPLETE™ Mini protease inhibitor cocktail (#11836153001) and phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: shRNA Endo180 knock-down clones from 3T3 and CAFs were generated using lentiviral particles (Sigma, Supplementary Table 3). For siRNA knockdown ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were fixed on day 0 and day 3 with 500 µl of 10% trichloroacetic acid (Sigma T9159) in 1 mL media and incubated at 4°C for at least one hour ...
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bioRxiv - Cell Biology 2021Quote: ... Calu-3 cells and differentiated macrophages were incubated with 25μM of merbromin (Mercury dibromofluorescein disodium salt, Sigma-Aldrich), 1μM of tannic Acid (Sigma-Aldrich ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... and construct expression was induced for 3 days using media above with 1 µg/mL tetracycline (Sigma Aldrich) added ...
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bioRxiv - Neuroscience 2020Quote: ... 6–8-week-old C57BL/6N mice were anesthetized by inhalation of isoflurane (3–4%) or intraperitoneal injection of 2% Avertin solution (2,2,2-tribromoethyl alcohol dissolved in Tert-amylalcohol (Sigma)) dissolved in saline ...
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bioRxiv - Physiology 2021Quote: ... 12 week-old mice were fed ad libitum and given CL316243 (3 mg/kg body weight; Sigma Aldrich) or vehicle (0.5% carboxymethylcellulose in sterilized water ...
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bioRxiv - Physiology 2020Quote: ... at 4°C for 60 min using an Amicon Ultra-4 3 kDa centrifugal filter device (Merck Millipore). The 50 μL retentate was the final volume of concentrated EVs ...
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bioRxiv - Biochemistry 2020Quote: ... Japan) and 1:3 mixture of PVA and polyvinylpyrrolidone (PVP: average MW, 40,000; Sigma-Aldrich, St. Louis, MO), respectively ...