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Citations for Millipore Sigma :
7401 - 7450 of 10000+ citations for 6 METHOXY 1 2 3 4 TETRAHYDRO NAPHTHALENE 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... One flask of cells was treated with 1-2 μM (98, 99) TAT-Cre Recombinase (EMD Millipore, #SCR508) while the other was treated with DPBS alone ...
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bioRxiv - Cell Biology 2022Quote: ... fixed with 2% paraformaldehyde in PBSP (1x PBS pH7.4, 1 mM DTT, 1x Sigma plant protease inhibitor cocktail) for 30 min on ice ...
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bioRxiv - Evolutionary Biology 2022Quote: ... corneas were resected and homogenized by incubation in 1mL of buffer containing 2 mg/mL collagenase 1 (Sigma) for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times and incubated in 4,6-diamidino-2-phenylindole (DAPI) (1 µg/ml; Sigma-Aldrich) for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were reduced and alkylated in digestion buffer containing 1:50 (v:v) tris(2-carboxyethyl)phosphine (0.5 M, Sigma) and 1:10 (v:v ...
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bioRxiv - Neuroscience 2022Quote: ... by 1:10 dilution in a vehicle solution 10% w/v 2-hydroxypropyl-β-cyclodextrin in PBS (Sigma). Alternatively ...
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bioRxiv - Microbiology 2022Quote: ... zebrafish embryos were treated with 180 µM of 1-phenyl 2-thiourea (PTU) (Sigma-Aldrich, 103-65-5), to prevent pigmentation [25] ...
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bioRxiv - Molecular Biology 2024Quote: ... 10-25 μg of chromatin was incubated with 1-2 μg of antibodies (Abcam: 9E10, Y69, ab2739, Millipore: 04-835 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by staining with a 1% alizarin red S solution (200 μL/well; Sigma-Aldrich, #01-2180-2) at pH6.4 ...
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bioRxiv - Plant Biology 2023Quote: ... and reduced and alkylated by addition of digestion buffer containing 1:50 (v:v) tris(2-carboxyethyl)phosphine (0.5 M, Sigma) and 1:10 (v:v ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey anti-rabbit immunoglobulin G secondary antibody (1:1,000 for 2 hours at RT; Millipore). To eliminate nonspecific binding prior to immunostaining endogenous peroxidase activity was blocked using 0.3% H2O2 for 30 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin in embryos was stained with Hoechst 33343 (1:5000 dilution in PBS, 2 μg/ml, Sigma-Aldrich) for 15 minutes.
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bioRxiv - Neuroscience 2023Quote: ... The eyes were then placed in a 2:1 solution of PolyFreeze Tissue Freezing Medium (TFM) (Sigma-Aldrich) and 30% sucrose for 24 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ40-E22Q was dissolved to 1 mM in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP; Sigma, St. Louis, MI, USA), incubated for 24h to breakdown pre-existing β-sheet structures and lyophilized ...
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bioRxiv - Cell Biology 2023Quote: ... containing 10 µg/ml LPC (leupeptin, Pepstatin A, and chymostatin) and 1% phosphatase inhibitor cocktail 2 (P5726, SIGMA). Following clarification of the lysate by centrifugation at 15,000 rpm (21,000 g ...
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bioRxiv - Immunology 2023Quote: ... Then the wells were washed 2 times with PBS and 200 μl of SLC3A2 (1: 500) (Sigma / HPA017980) primary antibody solution (in 5%BSA ...
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bioRxiv - Microbiology 2023Quote: ... P cells were grown till 0.5 OD600 in 2 ml LB with 1 mM deferoxamine mesylate (DFO, Sigma) or 1 mM FeCl3 respectively ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody65 (kind gift of G ...
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bioRxiv - Neuroscience 2024Quote: ... Both groups were treated with WIN55,212-2 (1 nM or 10 nM) (Sigma-Aldrich, St. Louis, MO, USA) dissolved in ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP (1:1000 ...
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bioRxiv - Systems Biology 2024Quote: ... 10 μg/ml leupeptin) and phosphatase inhibitors (10 mM NaF, phosphatase inihibitor cocktail 1 and 2, Sigma Aldrich). The cell lysate was treated with 5 mM dithiothreitol for 1 h to reduce proteins and then alkylated with 10 mM iodoacetamide for 45 min in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... NovaMatrix) was dissolved at 1% w/v in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES; Sigma-Aldrich), 0.3M NaCl (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... after which the cells were suspended (1 μl loop-full) in 2 ml R2A liquid medium (Sigma-Aldrich) supplemented with 0.5% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... To prevent pigmentation E3 medium was changed to 0.003% (m/v) 1-phenyl 2-thiourea (PTU; Sigma-Aldrich) at 24 hours post fertilization (hpf) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and incubated for 2 h in 1:20,000 horseradish peroxidase (HRP)-conjugated goat α-mouse IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Molecular Biology 2024Quote: ... re-suspended in ChIP Buffer 2 (10 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA (Sigma), Complete protease inhibitor cocktail ...
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bioRxiv - Bioengineering 2019Quote: ... cells were permeabilized via 0.02% Tween 20 for 20 minutes and incubated overnight at 4°C with rabbit anti-SLCO1B3 (OATP1B3) primary antibody (2-μg/mL working concentration, HPA004943, Sigma-Aldrich Canada, Oakville, Ontario, Canada). Goat anti-rabbit AlexaFluor 647-conjugated secondary antibody was then applied for two hours at room temperature (1:500 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with 15 μM substrate 2′ (4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-NANA) in 37°C warmed sterile filtered TBS (Sigma Aldrich; St. Louis, MO). Fluorescence intensity (excitation ...
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bioRxiv - Neuroscience 2023Quote: Mice were fasted for 2-5 hours prior to oral gavage of 4-kDa (Sigma-Aldrich, 600 mg/kg body weight, 80 mg/mL PBS) by gavage ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cells fixed with 4% paraformaldehyde (ProSciTech, Cat. no. C004) in PBS for 30 min at RT and blocked with 2% BSA (Sigma-Aldrich, Cat. no. A8022-10G) in PBS (BSA/PBA ...
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bioRxiv - Cell Biology 2020Quote: ... complete medium containing 20 mM 2-deoxyglucose (2-DG; Carl Roth GmbH, Karlsruhe, Germany) and 10 M antimycin-A (Sigma-Aldrich, Steinheim, Germany) for at least 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... the affinity gel was suspended in elution buffer (2% SDS, 50 mM Tris pH 7.5, 50 mM NaCl, 20% glycerol, 2 mM dithiothreitol (Sigma-Aldrich #D0632-5G; fresh) and incubated at 65°C for 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: The mouse was induced by injecting 2 month-old female BALB/c mice with2 mg human-derived proteoglycan (PG) together with 2 mg DDA (Sigma, MO, united States). as described previously (1) ...
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bioRxiv - Microbiology 2022Quote: ... and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2; Catalog no. H7041; Sigma, St. Louis, MO). All the cells were incubated at 37°C in the presence of 5% CO2.
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bioRxiv - Neuroscience 2022Quote: ... 1 hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in FACS buffer (1x DPBS, 2% FCS and 2 mM EDTA) + 5 μM Actinomycin D (ActD, Sigma, Cat#A1410-5MG) for transcriptomics ...
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bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
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bioRxiv - Microbiology 2023Quote: hPCLS were infected with SARS-CoV-2 at 2×105 TCID50 per slice and treated with homoharringtonine (HHT) (Sigma, cat# SML-1091-10MG) at 1 h p.i ...
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bioRxiv - Biophysics 2023Quote: Agarose gels were prepared at 2% (weight) in Tris-Borate-EDTA (TBE, Sigma-Aldrich, 89 mM Tris-borate, 2 mM EDTA, pH 8.3) buffer ...
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bioRxiv - Genomics 2023Quote: ... the following modifications were made to the initial extraction: 2% (w/v) polyvinylpyrrolidone (PVP, average molecular weight 40,000) and 10% 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO) were added to the lysis buffer ...
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bioRxiv - Physiology 2024Quote: ... 8.0x104 cells/well were seeded the night prior on a 96-well plate and treated with 5mM 2-deoxy-D-glucose 2h prior to the initial seahorse reading (2-DG; Millipore Sigma cat. D2134). ATP production rates from glycolysis (glycoATP ...
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bioRxiv - Developmental Biology 2024Quote: ... The following chemical compounds were prepared and used according to the manufacturers’ guidelines: 2-deoxy-D-glucose (2-DG, Sigma, cat. no. D8375); oligomycin-A (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1 % tetramethylethylenediamine (TEMED, Roth) and 0.005 % 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (4-hydroxy-TEMPO, Sigma) were added to activate the polymerization reaction ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were probed overnight at 4°C with anti-HA antibody (MBL, M180-3) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Sigma-Aldrich, G8795) or with anti-dystrophin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... 1:100 Phosphatase Inhibitor cocktail 3 (Sigma), 50mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... 1% Phosphatase Inhibitor Cocktail 3 (Sigma-Aldrich) and 0.5% Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... 1× Phosphatase Inhibitor Cocktail 3 (Sigma, P0044), 2 mM PMSF (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... 3-isobutyl-1-methyxanthine (Sigma Aldrich, I5879). Insulin (Actrapid Novonordisk ...
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bioRxiv - Bioengineering 2021Quote: ... 500μM 3-isobutyl-1-methyxanthine (Sigma Aldrich), 1μM pioglitazone (provided by AZ) ...