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Citations for Millipore Sigma :
7301 - 7350 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich).
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bioRxiv - Immunology 2021Quote: ... Sheared chromatin was “pre-cleared” using Protein A Agarose/Salmon Sperm DNA beads from Sigma-Aldrich. Pre-cleared chromatin from the equivalent of 3.57×106 cells was used in the chromatin IP reactions along 3μg of either anti-TCF1 (C63D9 ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were immunoprecipitated by the addition of 3 µl of rabbit anti-FGFR3 (Sigma-Aldrich, F0425)/500 µg protein with protein A-agarose (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... proteins were fractionated on SDS-polyacrylamide gel electrophoresis gel and transferred to a nitrocellulose membrane (Millipore). Membranes were incubated with primary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...
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bioRxiv - Neuroscience 2019Quote: ... Protein extracts were separated by SDS-PAGE and electroblotted to PVDF membrane (Millipore, Bedford, MA, USA). After blocking with 5% (w/v ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were visualized on Microchemi 4.2 (DNR Bio Imaging Systems) using Immobilion Western ECL reagents (Millipore).
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bioRxiv - Immunology 2021Quote: ... The cells were harvested by centrifugation and lysed by a BugBuster Protein Extraction Reagent (Novagen, USA). Nanobodies were purified by IMAC using an AKTA start system and a HisTrap column ...
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bioRxiv - Microbiology 2020Quote: ... anti-PP1b that cross-reacts with both PP1b isoform and PP1c isoform proteins (Millipore, 07-1217), anti-phospho-PP1a (Thr320 ...
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bioRxiv - Cell Biology 2019Quote: ... isolated PMNs were preincubated with 20μM TMB-8 (Ca2+ antagonist and protein kinase C inhibitor; Sigma), for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was filtered through a 0.45 µm low protein-binding membrane (Millipore Steriflip HV/PVDF). To achieve a 300 times concentration of the GeCKO pooled library ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were resolved by standard SDS-PAGE gels and transfer to Immobilon PVDF membranes (Sigma, #IPVH00010) and incubated with specific antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate protein concentration was determined using the Direct Detect Spectrometer (Millipore Sigma, Cat. No. #DDHW00010-WW) and normalized to 5 μg ...
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bioRxiv - Biophysics 2021Quote: Liposomes were separated from free protein on a Sepharose® CL-4B column (Sigma Aldrich #CL4B200). The conditions resulted in on average <<1 ctSTIM1 dimer per liposome.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on an SDS-PAGE gel and transferred to a PVDF membrane (Sigma-Aldrich). Membranes were blocked for at least 30 min in blocking solution (5% milk in 1X TBS-T ...
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bioRxiv - Neuroscience 2022Quote: RNA immunoprecipitation was performed using the Magna RIP® RNA-Binding Protein Immunoprecipitation Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The specific protein bands were visualized with immobilon western chemiluminescent HRP substrate (Millipore, Sao Paulo, Brazil), captured by Alliance 4.7 (Uvitec ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using an Amicon ultra-15 centrifugal filter unit concentrator (Millipore, Burlington, MA) and loaded onto an ion-exchange column (HiTrap SP FF ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Protein expression was visualized using Immobilon ECL Ultra Western HRP Substrate (Millipore Sigma, catalog number WBULS0500) in combination with the BioRad ChemiDoc Touch Imaging system ...
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bioRxiv - Cancer Biology 2022Quote: ... Non-specific protein interactions were blocked by incubating with 10% goat serum (Sigma-Aldrich Cat# S26) for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The column was calibrated with standard proteins of known Stokes radii (Sigma-Aldrich, St. Louis, MO). Abl2 or Abl2-557-C and tubulin were mixed with tubulin at a ratio of 1:4 and incubated for 30 min on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein-containing fractions were dialyzed in a cellulose membrane tube (14 kDa MWCO, Merck-Millipore), first ...
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bioRxiv - Pathology 2022Quote: ... The signal intensity of targeted protein was detected using ECL western blot detection solution (Millipore, WBKLS0500).
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bioRxiv - Molecular Biology 2022Quote: ... The precleared lysate was then transferred to Protein G Dynabeads coupled with anti-FLAG antibody (Sigma) and the mixture was incubated for 2 hours at 5°C on a roller ...
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bioRxiv - Microbiology 2022Quote: Wild type and mutant CANC proteins were expressed in Escherichia coli Rosetta (DE3) cells (Merck Millipore) as previously described (Betancor et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The presence of transferred proteins on the membrane was confirmed by staining with Ponceau S (Sigma). Membranes were blocked for 2 hours at room temperature with 5% non-fat milk in 0.1% tween-20/TBS (TBS-T) ...
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bioRxiv - Immunology 2022Quote: ... Proteins were resolved on SDS–PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The PVDF membranes were then blocked with 5% skim milk and sequentially incubated with primary and secondary antibodies ...
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bioRxiv - Genomics 2022Quote: In vitro translated protein was purified over 20 µL of anti-HA agarose beads (Sigma-Aldrich), glutathione agarose beads (GoldBio) ...
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bioRxiv - Biophysics 2022Quote: ... Five microliters of a 5 mg mL−1 solution of protein G (Sigma-Aldrich, Tokyo, Japan) was added to the flow chamber (about 5 μL) ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Immunology 2022Quote: ... coupled to protein A Sepharose magnetic beads (Ademtech 0423) and anti-TAX1BP1 antibody HPA024432 (Sigma-Aldrich) together with protein A Sepharose (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... 500–1000 µg of total protein (estimated by Direct Detect® Infrared Spectrophotometer assay, Merck Millipore), were incubated overnight with 2 µg of antibody raised against an intracellular epitope in mouse NLGN1 (aminoacids 826 to 843 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were harvested from the cells by subcellular fractionation using ProteoExtract subcellular fractionation kit (Millipore Sigma). Fractionated samples were analyzed by SDS-PAGE and electrophoresis for detection of HA-tagged Nedd4 (Roche anti-HA high affinity,1:2000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were fractionated on a 10% SDS-PA gel and transferred to PVDF membranes (Millipore, IPVH00010), which were then treated with 5% non-fat milk in 1× TBS buffer (50 mM Tris- HCl [pH 7.5] and 150 mM NaCl ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Diluted chromatin was pre-cleared with Salmon Sperm DNA/Protein A agarose slurry (Millipore, 16-157) at 50 μl slurry/ml of diluted chromatin in an end-to-end rotator overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Digested proteins were proceeded to SDS-PAGE and analyzed by silver staining (Sigma-Aldrich, PROTSIL2-1KT).
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bioRxiv - Microbiology 2021Quote: ... Protein was separated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, Millipore). Blots were blocked using 5% milk in Tris-buffered saline containing 0.1% Tween 20 (TBS-T) ...
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bioRxiv - Cancer Biology 2020Quote: ... which would allow controlled expression of FOXL2WT or FOXL2C134W protein by addition of Dox (Sigma, D9891). Replication-defective lentivirus containing the rtTA Advanced TET-ON was generated by co-transfection of pLVX-TET-ON ...
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bioRxiv - Plant Biology 2021Quote: ... The protein complexes were specifically eluted with 125 mkl of 100 ng/mkl 3xFLAG peptide (Sigma) solution in TBS during 30 min on-ice incubation with agitation ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... 60 μL (50% slurry) of Protein-RIPA-equilibrated FLAG-m2 beads (Sigma-Aldrich, Cat. No A2220) were added together with 1 μL of Benzonase (Merck-Millipore ...
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bioRxiv - Plant Biology 2021Quote: Proteins fractionated by gel electrophoresis were transferred to polyvinylidene difluoride membranes (PVDF) (Immobilon®-P, Millipore) using a semi-dry blotting system (Biorad ...
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bioRxiv - Genomics 2020Quote: ... except for the following modifications: immunoprecipitation was carried out using monoclonal antibodies against protein A (Sigma); the lysis buffer contained 10mg/ml heparin (sigma H7405) ...
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bioRxiv - Neuroscience 2020Quote: ... Following the estimation of protein concentration using the Quantipro BCA assay kit (Sigma-Alrich, United States), equal amounts of lysate were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel and then transferred onto polyvinylidene fluoride membranes ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitated antibody/chromatin complex was collected using Protein A/G magnetic beads (Millipore 16-663). The input and ChIP samples were reverse cross-linked overnight at 45°C using 0.2M final concentration of NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...