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Citations for Millipore Sigma :
7301 - 7350 of 10000+ citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Corresponding secondary HRP-conjugated anti-rabbit (1:3000 or 1:2000 in 5% non-fat milk or 5% BSA/TBS-T; Sigma-Aldrich, St. Louis, MO, USA) was used for a 1-hour incubation at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated at 4 °C overnight in the same solution with the primary rabbit anti-OMP (olfactory marker protein; 1:500, O7889 from Sigma). Immunofluorescence was achieved by reaction with the secondary antibody donkey anti-rabbit-488 (A21206 from Molecular Probes ...
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bioRxiv - Immunology 2019Quote: ... saturated in blocking solution (PBS-BSA 5%) for 1 hour and finally incubated with primary Abs: rabbit anti-mouse laminin (Sigma), Alexa fluor 594 Goat anti-mouse IgM (Thermofisher ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... the NC membranes were incubated with goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP; Sigma-Aldrich; dilution, 1:8000) and the color reaction was examined using ECL Kit (Amersham Pharmacia Biotech ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was then stained and visualised for β-actin (rabbit anti-β-actin HRP, Sigma-Aldrich, A3854, 1:50,000) as described above.
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bioRxiv - Biochemistry 2021Quote: ... or 680LT-conjugated anti-rabbit IgG (1:5000, Li-COR) and IRDye 800CW (Li-COR) labeled HPA (Helix pomatia lectin, Sigma) or PNA (peanut agglutinin ...
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bioRxiv - Microbiology 2021Quote: ... The deposited C5b9 complex in the reaction mixture was detected using rabbit anti-C5b-9 pAb at 1:1000 dilution (204903; Sigma) and HRP conjugated anti-rabbit pAb (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated with 50 µl of goat anti-rabbit IgG conjugated to alkaline phosphatase diluted 1:2000 (A3687, Sigma) in PBS for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in the dark in Alexa 555-conjugated or Alexa 488-conjugated goat anti-rabbit-IgG (1:300; Millipore Sigma) for 2 h following primary antibody incubation ...
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bioRxiv - Pathology 2019Quote: ... Western-blot analysis was carried out using anti-LC3-II (1:1000, rabbit polyclonal, Sigma-Aldrich, Saint-Quentin Fallavier, France), anti-ß-tubulin (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... the sections were washed (3 × 5 min) in 0.1 M PBS and incubated with goat anti-rabbit Alexa488 (1:500; Sigma-Aldrich) secondary antibody (to label collagen-I ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C with rabbit anti-myc (1:250; Sigma-Aldrich Cat# C3956, RRID:AB_439680) in PBS with 2% BSA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were then washed again and incubated with horseradish peroxidase-conjugated rabbit anti-horse IgG (1:1000; Sigma, UK) for 3 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... VP sections near the center of the microinjection sites were incubated overnight at room temperature in rabbit anti-Fos (1:5000; Millipore) and mouse anti-DSRed (targeting mCherry ...
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bioRxiv - Neuroscience 2021Quote: ... fixed with a 4% PFA solution and stained for tyrosine hydroxylase (TH; rabbit anti-TH, 1:1000, Cat#: 657012, Merck Millipore) and neurobiotin (Streptavidin Alexa Fluor conjugate 647 ...
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Free-floating sections were incubated at 4°C for 16 hours with rabbit anti-Myc (1:500, C3956; Sigma-Aldrich) primary antibody in PBS/0.5% normal donkey serum/0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... protein isolation and immunoprecipitation was as previously described (Mohammed et al., 2016) using 1×107 OL-differentiated NSCs incubated with rabbit anti-OLIG2 (AB9610, Millipore) and using rabbit pre-immune IgG as control ...
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bioRxiv - Neuroscience 2022Quote: ... and 3% BSA followed by overnight incubation at room temperature with rabbit anti-KCC2 IgG (1:1000 dilution; #07-432, Merck-Millipore) (Yassin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were then washed 3 times for 20 min in PBS-Triton before incubation with anti-rabbit Alexa Fluor 546 used at 1:500 dilution in blocking buffer containing Hoechst 33342 (Sigma) to label the nuclei ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed with PBS-0.1% Tween 20 and then incubated with 100 μl of 100 μg/well of biotinylated rabbit anti-human C1q (Sigma) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated with 1:5 diluted Duolink® in Situ PLA Probe anti-rabbit Plus (cat#DUO92002, Sigma-Aldrich) and anti-mouse Minus (cat#DUO92004 ...
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bioRxiv - Systems Biology 2020Quote: ... Antibodies used included: Rac1 antibody (Millipore, clone 238A, 1:500), anti-RhoA antibody (Santa Cruz Biotechnology 26C4 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μL of antibody (α-Flag-M2 antibody, Sigma-Aldrich / α-HA ...
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bioRxiv - Neuroscience 2019Quote: ... 1:1,000 for chicken anti-GFAP, Abcam; 1:1,000 for anti-Iba-1, Wako; 1:1,000 for anti-TH, Millipore). Sections were then washed in PBST and incubated for 1h at room temperature with the corresponding Cy-(1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... GluK2/3 (rabbit, Millipore, 04-921, WB 1:4000, IF 1:500), Homer (guinea pig ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies were rabbit anti-phosphorylated ERK (1/500; Tasaki et al., 2011a) or mouse anti-α-tubulin monoclonal antibody DM 1A (1/5,000; Sigma-Aldrich, T9026). An appropriate antibody conjugated with horseradish peroxidase (1/5,000 ...
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bioRxiv - Neuroscience 2022Quote: ... whereas transfected Flag-Glt-1 cells were incubated overnight with a mouse anti-Flag® primary antibody (1:200-Sigma Aldrich; #F1804) that recognized the DKK sequence ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies and their concentrations were as follows: 1:1000 mouse-anti-α-SMA (Millipore Sigma; Burlington, MA; clone ASM-1/1A4), 1:300 rabbit-anti-Daam1 (Proteintech ...
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bioRxiv - Molecular Biology 2019Quote: ... Following fixation with cold methanol and blocking with 1% BSA, presence of RAGE in the nucleus was confirmed using immunofluorescence (primary antibody, 1:200 goat anti-RAGE (Millipore, CA, USA), 1:5000 secondary antibody donkey anti-goat 488 (LICOR ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:400 mouse anti-light chain clathrin antibody (Sigma Aldrich, C1985) in PBS + 1% BSA and washed three times with PBS + 1% BSA ...
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bioRxiv - Biophysics 2019Quote: ... Then they were incubated again for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1% BSA ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and NURF-1.F isoform with FLAG tag was detected using 1:1000 Millipore ANTI-FLAG antibody (Millipore Sigma, Catalog number: F3165).
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bioRxiv - Bioengineering 2022Quote: Specimens were permeabilized with 4% paraformaldehyde and incubated for 1 hour at room temperature in primary antibody anti-vinculin (1:500, Sigma Aldrich, V4505), secondary antibody Alexa Fluor 488 (1:600 ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS at 37°C for 30 minutes and subsequently incubated for two hours with a 1:200 concentration anti-glial fibrillary acidic protein (GFAP) antibody produced in mouse (Sigma, 1:200) and 1:500 concentration anti-β-Tubulin III antibody produced in rabbit (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... All four antibodies were used together (1:200) for staining protocols in sequential stainings with Chicken anti-neurofilament 200 (NF200, Millipore, 1:1000), Rabbit anti-S100 (Dako ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was subsequently blocked using 1% casein in 1x PBS and incubated with primary antibody solutions with following dilutions: mouse anti-HA (Sigma, 1:500), rabbit anti-HSP101 (r950 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated with primary antibodies in blocking buffer in a humidified chamber for 1 h: anti-FLAG M2 (1:2000 dilution, Sigma Aldrich, F3165) and an endogenous compartment marker antibody from rabbit (Supplementary Table 21 for list of antibodies used) ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were used at 1:1000 and included: mouse monoclonal anti-β1/β2-adaptins (clone 100/1; Sigma, St. Louis, MO); mouse monoclonal anti-clathrin heavy chain (clone 23 ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... then washed twice in PBS and incubated 1 hour with the tertiary antibody goat anti-mouse whole molecule conjugated to FITC (Sigma, 1:400). The intrabody anti-GFP nanobody 3 x HA-tagged (INbGFP::3HA ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated for 1 hour at room temperature with a goat anti-mouse alkaline phosphatase-conjugated secondary antibody (dilution 1: 5000; Sigma-Aldrich, USA) and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight at 4 °C with BB containing a primary antibody cocktail (1:150 mouse anti-ionized calcium-binding adapter molecule 1 (Iba1; EMD-Millipore cat# MABN92+), 1:300 rabbit anti-transmembrane protein 119 (TMEM119 ...
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bioRxiv - Cell Biology 2022Quote: ... to block non-specific staining and incubated with a mouse anti-acetylated tubulin antibody (Clone 6-11b-1, 1:100; Sigma Cat# T6793) for 4 h at room temperature ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked in 1% Casein/PBS (1 hour, room temp) and incubated with the following primary antibodies (4°C, overnight): anti-Nup93 (1:1000, Sigma-Aldrich, HPA017937); anti-Nup107 (1:200 ...
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bioRxiv - Biochemistry 2024Quote: ... Excess serum was tipped off and sections were incubated with primary antibody (anti-FLAG, Sigma F7425, 1:100 diluted in 1% BSA), overnight in a humid chamber at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The following primary antibodies were applied for 24 h (or 48 h for anti-cFos antibodies) at 4°C: mouse anti-APC/CC1 Ab-7 (Millipore, OP80, 1:100), chicken anti-mCherry (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Blot was probed with ∼1:10,000 diluted primary antibody (anti-Flag™ for ParA-M2, F7425 Sigma-Aldrich, and anti-DnaA for DnaA) overnight at 4°C ...