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Citations for Millipore Sigma :
6951 - 7000 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... All samples were diluted to the same protein concentration using 2X Laemmli buffer (Sigma-Aldrich), boiled for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mg lysate proteins were mixed with 200 µL of anti-Flag M2 (Sigma-Aldrich) or anti-HA (Thermo Scientific ...
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bioRxiv - Genetics 2024Quote: ... Proteins were transferred to either NitroBind nitrocellulose (GE, EP4HY00010) or Immobilon-FL PVDF (Millipore, IPFL00010) membranes in Tris-Glycine transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... 5-100 ng of S-RBD protein (SinoBiological, 40592-V08B) or ovalbumin (Sigma-Aldrich, A5503) recombinant protein were added as control ...
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bioRxiv - Immunology 2024Quote: ... and RIP assays were carried out with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore) and anti-Flag antibody (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... the protein was concentrated to approximately 30 mg/ml using an Amicon Ultracel-10K (Millipore) and then subjected to size-exclusion chromatography using either an S75/26–60 column or an S200/26–60 (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein was extracted using RIPA buffer mixed with protease inhibitor cocktail (Millipore Merck, 539134) and quantified using Qubit® Protein Assay Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to 0.45 µm pore size polyvinylidene difluoride membranes (PVDF) (Millipore, catalog # IPVH00010), incubated with rabbit anti-LC3B antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Medium was supplemented with 10 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Sigma) when iPSCs were thawed or passaged as single cells.
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bioRxiv - Immunology 2023Quote: ... protein bands within the gel were transferred on an Immobilon-FL transfer membrane (Millipore IPFL00010) for 45 mins at 20 volts and 400 mAMPs ...
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bioRxiv - Plant Biology 2023Quote: ... was used to detect proteins with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (Sigma, A6154).
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bioRxiv - Synthetic Biology 2022Quote: ... The buffer of the eluted protein was subsequently exchanged using a G25 column (Sigma-Aldrich) into TEV buffer (50 mM Tris ...
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bioRxiv - Pathology 2023Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were lysed in protein lysis buffer (Cell Signalling) supplemented with phosphatase (Phos-Stop, Sigma) and protease (Complete Mini ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested with a combination of LysC (Wako Chemicals) and Trypsin (Porcine, Sigma Aldrich) (1:70 and 1:25 enzyme to protein ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration in the extracts was measured using the QuantiPro BSA Assay Kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated via 15% SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore). Western blot analyses were then conducted using PilA- ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualized with the FITC- and TRITC-conjugated secondary antibodies (Sigma, F6005, and T5393) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was induced with 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich, I6758) for 2 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma, Merck) in 50 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Systems Biology 2022Quote: Single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was estimated using 4% copper sulfate and bicinchoninic acid (Sigma, USA, Cat#B9643). Protein samples were separated by 8-12% SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... The amount of total protein in the samples was quantified using the Bradford assay (Sigma). An equal concentration of protein samples was mixed with 2X Laemmli buffer (BioRad) ...
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bioRxiv - Plant Biology 2023Quote: ... After electrophoresis proteins were transferred to a Polyvinylidene fluoride (PVDF; Immobilon-P™, Millipore®) membrane using semi-dry blotting (Amersham biosciences TE 77 ECL Semi-Dry Transfer Unit ...
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bioRxiv - Physiology 2023Quote: ... STIM-1 polyclonal (Protein Tech group) was used at 1:250 and HCN4 polyclonal (Sigma) was used at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Total proteins of BR-EVs were extracted with RIPA buffer containing 0.5% PMSF (Sigma Aldrich). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... to stain adrenergic nerve fibers and growth association protein 43 (GAP-43, Millipore, #AB5220;1:100) for regenerating nerve terminals ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 0.5ml using Amicon Ultra-4 ml Centrifugal Filters (Millipore) and loaded onto a gel filtration column (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was precipitated after chilling samples on ice and adding cold ammonium acetate (Sigma, A1542) to a final concentration of 1.875 mM ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of protein was incubated with 20 mM methoxypolyethylene glycol maleimide (mPEG, Sigma-Aldrich) in the presence of 3% SDS for 10 minutes at 60ºC ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Developmental Biology 2023Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Biophysics 2023Quote: ... Protein was eluted with wash buffer supplemented with 100 mg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to a storage buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins reacting with primary antibodies were visualized by the Immobilon forte western HRP substrate (Millipore) for detecting peroxidase-conjugated secondary antibodies and analyzed with an ChemiDoc Touch (Bio-rad).
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were expressed in Escherichia coli strain BL21(DE3) or Rosetta 2 (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to FL-PVDF membranes (cat. No. IPFL00010; Merck Millipore; Massachusetts, USA). The membranes were incubated with ms anti-Y1-R (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Microbiology 2023Quote: ... The protein bands were developed with Immobilon Forte Western HRP substrate (Millipore Sigma, MA, USA) and imaged using a Bio-Rad ChemiDoc Imaging System (Hercules ...
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Microbiology 2023Quote: ... Dried protein pellets were resuspended with 50 mM TEAB buffer (Millipore Sigma, Burlington, MA, USA) and quantified using Pierce 660nm Protein Assay Reagent (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to an Immobilon-P membrane (Millipore, USA) for 30 min using the Towbin’s transfer buffer in a semi-dry transfer system ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were fractionated by SDS-PAGE and transferred to 0.4 µm PVDF membranes (Millipore, MA) prior to immunoblotting ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated protein (7-15 mg/ml) was serially diluted in 1% Genapol X-100 (Sigma). 0.3-0.5 μl of diluted protein was added to the cis (ground side ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were purified from clarified supernatants using a 1mL StrepTrap™ HP column (Sigma), buffer exchanged ...
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bioRxiv - Plant Biology 2023Quote: ... The separated protein samples were transferred to PVDF membranes (Immobilon-P; Millipore, Burlington, MA, USA) using the Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).