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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Synaptic Ras GTPase Activating Protein 1 Syngap1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... 100uL of Pierce Protein A/G Magnetic Beads were coupled to 10 µg of antibody or control rabbit IgG antibody (Sigma). DNA was incubated with antibody-coupled beads and after uncoupling ...
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bioRxiv - Microbiology 2024Quote: ... Solution containing primary antibodies for FLAG-tagged proteins are made by adding Anti-FLAG M2 antibodies (produced in mouse) from SIGMA into 10mL of the blocking solution (1:10000) ...
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bioRxiv - Microbiology 2024Quote: ... Solution containing primary antibodies for HA-tagged proteins are made by adding Anti-HA HA-7 antibodies (produced in mouse) from SIGMA into 10mL of the blocking solution (1:20000) ...
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Retinoic acid (RA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) used for cell differentiation were purchased from Sigma-Aldrich (R2625 and P8139, respectively). According to our previous work (Carmona et al. ...
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bioRxiv - Immunology 2021Quote: RA was induced in the SKG SPF mice by intraperitoneal (IP) injection of 2 mg zymosan A (Sigma-Aldrich, St Louis MO; Z4250). These animals develop arthritis within 2-3 weeks of the zymosan A injection ...
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bioRxiv - Immunology 2023Quote: ... Serotype EH100 (Ra) (TLRgrade™) – Enzo Life Sciences #ALX-581-010-L002) at 10 ng/ml and/or dexamethasone dissolved in DMSO (Sigma Aldrich # D8893) at 100 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... The differentiation protocol consisted of two stages: a 4-day pre-differentiation step in EMEM/F-12 supplemented with 10% FBS and 10uM RA (Sigma-Aldrich, Cat# R2625), and a subsequent 6-day differentiation step in serum-free medium containing 50 ng/ml human brain derived neurotrophic factor (BDNF ...
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bioRxiv - Neuroscience 2024Quote: ... The differentiation protocol consisted of two stages: a 4-day pre-differentiation step in EMEM/F-12 supplemented with 10% FBS and 10uM RA (Sigma-Aldrich, Cat# R2625), and a subsequent 6-day differentiation step in serum-free medium containing 50 ng/ml human brain derived neurotrophic factor (BDNF ...
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bioRxiv - Bioengineering 2021Quote: ... 43] using rabbit polyclonal antibody Synapsin-1 (SYN-1, 1:200; Millipore), goat anti-guinea pig antibody VGlu2 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... or 1/200 anti-RpsF (Dundee Cell Products) antibodies and 1/6000 anti-rabbit secondary antibody (Sigma). Bound antibody was visualized using ECL chemiluminescent detection reagent (GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: ... CC1 (rabbit polyclonal antibody, 1:500, Oasis Biofarm, OB-PRB070, mouse polyclonal antibody, 1:300, Millipore, OP80), ASPA (rat polyclonal antibody ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used as primary antibodies: mouse anti-vinculin (1:400; clone hVIN-1, Sigma), rabbit anti-non-muscle myosin heavy chain II-A (NMIIA ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed in PBS-T for 40 min and the bound proteins were visualized with anti-FLAG-HRP antibodies (1:10000 in PBS-T for 1h, Sigma-Aldrich, Cat. no: A8592). All steps were performed at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled RNAs in the eluate were then detected by autoradiography and proteins were subjected to WB using an anti-Flag antibody (Sigma-Aldrich F3165; 1:10000).
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Cells were then fixed using 10% formalin and processed for immunofluorescence using an antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) and a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). The following day ...
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bioRxiv - Neuroscience 2022Quote: ... fast synaptic glycinergic and GABAergic inhibition was blocked by bath application of strychnine (1μM; Sigma-Aldrich), and picrotoxin (50μM ...
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bioRxiv - Neuroscience 2021Quote: ... GABAergic and glutamatergic synaptic input was reduced by addition of 0.4 mM picrotoxin (P1675; Sigma-Aldrich), 50 µM D-AP5 (A5282 ...
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bioRxiv - Neuroscience 2023Quote: ... Excitatory and inhibitory synaptic currents were blocked using 20 µM 6,7-dinitroquinoxaline-2,3-dione (DNQX, Sigma) and 10 µM SR95531 (Abcam) ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2, 1:100, Millipore; mouse anti-GRIN2BB ...
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bioRxiv - Plant Biology 2022Quote: Protein phosphorylation was performed by incubating 1 µg of PpeAld6PRase or myelin binding protein (MBP; Sigma-Aldrich) with the corresponding protein kinase in a standard medium containing 20 mM Tris-HCl pH 7.2 ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated in primary antibody of anti-HCN4 antibody (1:200, Alomone Labs, Israel) and anti connexin43 antibody (1:500, Sigma-Aldrich CO, US) for 3 nights at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-TUJ-1 antibodies (1:200; Merck-Millipore, MAB380) for 48 h ...
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bioRxiv - Zoology 2020Quote: ... We used anti-UCP-1 antibody (Sigma, U6382; 1:1000) and HRP-conjugated anti-rabbit IgG secondary antibodies (CST ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies (1:250 Anti-Fibronectin-1, F3648, Sigma-Aldrich; 1:100 Goat Anti-Type I Collagen ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were: rabbit anti-Barhl1 polyclonal antibody (Atlas Antibodies, HPA004809, Sigma, 1:500), goat anti-Robo3 polyclonal antibody (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies used: Antibody anti-acetylated tubulin (clone 6-11B-1, Sigma), α-tubulin (Biorad MCA77G) ...
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bioRxiv - Molecular Biology 2020Quote: ... and probed with primary antibodies: anti-RPL26 antibody (1:1,000; Sigma R0780), anti-NSUN2 (1:1,000 – 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were alkaline phosphatase-coupled IgG antibodies (1:10000; Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: Primary antibodies included: mouse anti-PCNA monoclonal antibody (1:500; Sigma Aldrich), mouse anti-glutamine synthetase (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... APC-035) and GFAP antibodies (GFAP clone antibodies #MAB360; 1:200, Millipore) for 24 hrs at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibody anti-MPO antibody (rabbit, dilution 1:10,000, Sigma-Aldrich, HPA021147) was added and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... using as primary antibody mouse monoclonal antibody 6-11B-1 (Millipore Sigma) diluted 1:50 with PBS ...
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bioRxiv - Pathology 2024Quote: ... Primary antibodies were used as follows: anti-Tuj-1 antibodies (mouse, Sigma, T8860 ...
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bioRxiv - Biophysics 2021Quote: ... Protein bands were investigated by using anti-αS monoclonal antibody (Syn 211, Millipore Sigma) and anti-mouse horse radish peroxidase secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... antibodies and precipitated proteins were analyzed by western blot using mouse anti-HA (Sigma) or mouse anti-Flag (Sigma) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Plant Biology 2020Quote: ... total proteins were incubated with 50 µL agarose conjugated with anti-Myc antibody (Sigma) for 2 h ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...