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Citations for Millipore Sigma :
651 - 700 of 4382 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Neuroscience 2019Quote: ... GABAA receptor α subunit (1:250; rabbit; Sigma-Aldrich, St. Louis, USA; Cat# G5544), MAP2 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... we applied either dopamine or the D2-like receptor antagonist eticlopride (Sigma-Aldrich Spain) iontophoretically through multi-barreled pipettes attached to the recording electrode ...
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bioRxiv - Neuroscience 2020Quote: ... a mouse (IgG2a) anti-GluA2 (Glutamate receptor subunit A2) at 1:2000 (#MAB397, Millipore) a chicken anti-neurofilament (AB5539 ...
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bioRxiv - Neuroscience 2019Quote: ... or an equal amount of normal IgG (3 μg, androgen receptor Assay Kit, Millipore). After decrosslinking and DNA purification ...
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bioRxiv - Cell Biology 2020Quote: ... Receptors were surface labelled by addition of M1 anti-Flag antibody (1:1000, Sigma) conjugated to Alexa 555 (A10470 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit (rb)-α-gamma-aminobutyric acid receptor α6 (GABARα6; 1:500; Millipore Sigma; #AB5610), rb-α-T-box brain protein 2 (Tbr2 ...
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bioRxiv - Cell Biology 2022Quote: ... receptor independent activator FSK (1 or 10 μM, Sigma-Aldrich, St. Louis, MO, USA) was added 20 h after activation and differentiation treatments ...
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bioRxiv - Neuroscience 2022Quote: ... anti-platelet derived growth factor receptor alpha (PDGFRa) (1:100, rat, Millipore Cat# CBL1366), anti-GFP (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... a glucocorticoid receptor agonist and 1 mg/mL mifepristone (RU-486, M8046, Sigma Aldrich), a glucocorticoid receptor antagonist ...
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bioRxiv - Neuroscience 2023Quote: ... 5-HT receptor antagonist (10-5M methiothepine mesylate salt, Sigma, CAS:74611-28-2), 5-HT1A receptor antagonist (10-5M WAY-100635 maleate ...
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bioRxiv - Neuroscience 2022Quote: Pharmacological experiments with the GABAA receptor blocker gabazine (SR 95531 hydrobromide, Sigma-Aldrich, #104104509), were performed on sparse (50,000 per well ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted receptors were incubated onto a Flag affinity resin (Anti-Flag M1, Sigma) for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and the D1 receptor antagonist ecopipam were purchased from Sigma Aldrich (St. Louis, MO), were dissolved in 0.9% NaCl and administered IP before the behavioral testing (haloperidol ...
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bioRxiv - Immunology 2023Quote: ... or the A1 ADO receptor (ADORA1) antagonist 8- cyclopentyl-dipropylxanthine (DPCPX) (10 μM) (Sigma) were added to the medium in the absence of or together with MTX.
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bioRxiv - Neuroscience 2024Quote: ... and rat anti-muscarinic ACh receptor m2 (M2R) antibody (1:500; MAB367, Sigma-Aldrich) at 4 °C for 24 h on a shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Estrogen receptor antagonist ICI 182,780 (0.5 mg/mouse, Sigma Aldrich, #V900926, St. Louis, MO) was administered 1 hour before E2 injection to block all estrogen receptors41 ...
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bioRxiv - Molecular Biology 2020Quote: ... and FLAG-tagged ribosomes were purified using M2 FLAG-affinity resin (Sigma), as described previously (Inada et al. ...
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bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
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bioRxiv - Genetics 2020Quote: ... anti-mouse secondary antibody tagged with alkaline phosphatase (Sigma, catalogue no: A3562), CDP star (sigma) ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma). Immunoprecipitates were incubated for at least 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... FLAG-tagged TSC2 was immunoprecipitated with ANTI-FLAG® agarose beads (Sigma) for 2 hours at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... HA-tagged proteins were recognized using a murine antiHA antibody (Sigma-Aldrich) and then an antiMouse IgG-peroxydase antibody (KPL) ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP tagged proteins was captured using IgG sepharose fast flow beads (Sigma) and proceeded as described (33) ...
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bioRxiv - Biochemistry 2019Quote: ... Strep-II-tagged proteins were bound to Strep-Tactin resin (EMD Millipore), washed with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged ZC3H11A was immunopurified with anti-Flag(M2) magnetic beads (Sigma) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... or control plasmid expressing 3xFLAG-tagged TRIM25 through X-tremeGENE9 (Millipore Sigma). Twenty-four hours later ...
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged protein signals were detected with anti-FLAG (Sigma-Aldrich, F1804) in combination with anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP-tagged anti-rabbit and anti-mouse secondary antibodies (SA) (Sigma-Aldrich). Densitometry values of the immunoblots were computed using GelQuant.Net software.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... HA tagged CPα was probed using a mouse anti-HA antibody (Sigma). Probed proteins were detected using donkey anti-rabbit IgG antibody ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with FLAG-M2 antibody (F1804 Sigma-Aldrich) conjugated to protein G dynabeads (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... After incubation with peroxidase-tagged secondary antibodies (1:2000 diluted, Sigma-Aldrich), membranes were revealed with an ECL-plus chemiluminescence western blot kit (Amershan-Pharmacia Biotech).
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... followed by HRP-tagged anti-rabbit secondary antibody (1:10,000, Sigma-Aldrich).