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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Recombinant Human CSF2 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Biophysics 2022Quote: ... Non-specific protein adsorption to the coverslip was prevented by adding 0.005% (v/v) Tween-20 (P2287, Sigma-Aldrich) to the solution ...
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bioRxiv - Cancer Biology 2019Quote: ... a sample mixture of 10 μg protein and non-reducing sample buffer was loaded on a polyacrylamide gel containing gelatin (0.25 mg/ml; Sigma). The gel was then soaked (36 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
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bioRxiv - Immunology 2020Quote: ... human TLR4/CD14/MD2 or non-transfected (null-1) were cultured and maintained using DMEM GlutaMAX™ containing 10 % FBS (Sigma-Aldrich), 1 % P/S ...
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bioRxiv - Cancer Biology 2021Quote: ... Either DNA from the BRCA1-methylated HCC38 TNBC cell line or CpGenome™ Human Methylated and Non-Methylated DNA Standards (Sigma-Aldrich) were included in each bisulfite conversion reaction as controls ...
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bioRxiv - Microbiology 2020Quote: ... 100 μg/ml of biotinylated HA (Sigma) was added to each well and incubated for 1 h at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... stored and biotinylated using biotin-NHS (Sigma) as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... or 1:1500 biotinylated IB4 (Sigma, L2140) while shaking at 4 °C overnight in solution with 0.2% Triton X-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... Biotinylated porcine intestinal mucosa heparin (Merck Millipore) was immobilized on streptavidin biosensors at 10 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... biotinylated WFA (1:1000, Sigma, cat# L1516), rabbit anti-BCAN (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... IB4 biotinylated (Sigma Aldrich, L2140, 1:200). Sections were washed in PBS for 5 minutes three times ...
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bioRxiv - Neuroscience 2023Quote: ... and biotinylated WFA (1/100, Sigma L1516). Secondary antibodies were Alexa Fluor-conjugated (Molecular Probes) ...
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bioRxiv - Immunology 2023Quote: ... OVOL1 protein expression were determined by rabbit anti-human OVOL1 antibody (Sigma Aldrich).
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Bioengineering 2023Quote: ... 0.5 wt% non-allergenic and non-toxic methylcellulose (Sigma Aldrich, St. Louis, MO, USA) was added to the serum-free DMEM culture media ...
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
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bioRxiv - Cell Biology 2022Quote: ... non-essential aminoacids (Sigma-Aldrich), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and non-radioactive ZnCl2 (Sigma). Uptake was halted by applying the same volume of ice-cold stop buffer (15 mM HEPES ...
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bioRxiv - Cancer Biology 2020Quote: ... non-essential amino acids (Sigma), 0.15 mM monothioglycerol (MTG) ...
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bioRxiv - Molecular Biology 2022Quote: ... non-essential amino acids (Millipore), L-glutamine (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... non-targeting (Millipore Sigma, SHC016) and Pink1 (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... non-essential amino acids (Sigma), and L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... non-essential amino acids (Sigma), sodium pyruvate (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... non-essential amino acids (Sigma), penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... non-essential aminoacids (Sigma-Aldrich), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... GST tagged was bought from Sigma-Aldrich (SRP5172). G-actin and biotinylated actin were bought from Cytoskeleton ...
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bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-goat secondary antibody (Sigma-Aldrich), GFP ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-mouse FITC tagged (1:200, Sigma) at room temperature in dark for 90 min ...
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were loaded to have roughly similar band intensity for the band of interest to enable better estimation of relative protein size (recombinant pro-conA: 0.75 μg, conA (Sigma): 1.5 μg ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was used as the host for expressing recombinant proteins encoded in the pET30a vector (Novagen, USA). Appropriate antibiotics were added when needed at the following concentrations ...
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...