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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... for each IP reaction 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of GFP antibody (mouse anti-GFP ...
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bioRxiv - Cell Biology 2020Quote: ... 1-5ug of antibody was incubated overnight with chromatin and the complex antibody-chromatin was then captured with G protein sepharose beads (Sigma Aldrich) 1h at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and the supernatant containing the sheared chromatin was pre-cleared with Protein G plus-Agarose beads (Cat No. IP04-1.5ML, Merck Millipore, Burlington, USA), which were pre-coated with bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then added to the Protein G sepharose beads coupled to either HA 12CA5 mouse monoclonal IgG (Sigma Aldrich) or anti-Ty1 (BB2 ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with protein A/G agarose beads (Pierce, Waltham, MA, USA) conjugated to antibodies against FLAG-tag (Sigma-Aldrich) or Exo70 (Santa Cruz ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... determined by BCA assay were precleared for 1 hour with Protein G Sepharose 4 Fast Flow beads (GE17-0168-01, Millipore Sigma) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A-Sepharose and Protein G-Sepharose were purchased from Sigma-Alderich ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... Anti-Flag IP was performed by incubating 1 mg lysate with anti-Flag M2 conjugated beads (Sigma) for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... They were then incubated with antibody-bead conjugate (Anti-FLAG® M2 Magnetic Beads, Millipore & Sigma or GFP-Trap_A, Chromotek) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... They were then incubated with antibody-bead conjugate (Anti-FLAG® M2 Magnetic Beads, Millipore & Sigma or GFP-Trap_A, Chromotek) overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were measured in spinal cord homogenates by Luminex multiplex bead-based analysis (MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel; Millipore) using the Bio-Plex 200 system (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were clarified by ultracentrifugation and supernatants were supplemented with NaCl up to a final concentration of 500 mM and subjected to anti-FLAG immunoprecipitation using 100 ul of packed beads magnetic beads (anti-FLAG, M2, Sigma). After 16h beads were washed 5 times with IP buffer and eluted twice with 250 ul of 1 mg/ml 3x-FLAG peptide (Bachem ...
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bioRxiv - Neuroscience 2023Quote: ... Cytokine levels were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) using the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Microbiology 2024Quote: ... Purified lysates were co-immunoprecipitated by incubation with 50 µL packed bead volume of Anti-FLAG M2 magnetic beads (Sigma) for 1 h at 4°C by slightly rotating ...
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bioRxiv - Cell Biology 2020Quote: ... Approximately 0.4 g of glass beads (425 - 600 μm, Sigma) was added to the mixture ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 g of glass beads (acid washed, 1 mm, Sigma) were added and the cells were disrupted in a MiniBeadbeater-24 (Bio Spec Products Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... lysed with the addition of glass beads (G-8772, Sigma) and 5 X 1 minute disruption in Mini-Beadbeater-8 (Biospec Products ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 g of glass beads (0.106 µm diameter, Sigma-Aldrich) were added to the mixture and the pellet was resuspended by bead beating using the FastPrep-24™ 5G bead beating grinder and lysis system from MP at a speed of 6.5 m/sec for 40 seconds ...
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bioRxiv - Cell Biology 2020Quote: Mouse of 5-8 weeks of age were injected intraperitoneally (IP) with 1 µg/g of Tunicamycin (Sigma) using 150 mM of D-Glucose as vehicle ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates were either incubated with Anti-FLAG® M2 Magnetic Beads (Millipore Sigma) overnight with continuous rotation at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... the crude lysates were incubated with Anti-FLAG M2 Magnetic Beads (SIGMA-ALDRICH, M8823) or Anti-NP Magnetic Beads (MCE Protein A/G Magnetic Beads and NP antibody from our lab ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell extract were applied to magnetic anti-Flag M2 beads (M8823; Sigma-Aldrich) and incubated for 2 hours at 4°C with rotation ...
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bioRxiv - Microbiology 2020Quote: ... Co-immunoprecipitation was performed according to manufacturer’s instructions (Anti-Flag Magnetic Beads, Sigma-Aldrich). For co-immunoprecipitation of endogenous proteins 5×106 bone marrow derived macrophages were infected with CHIKV at a MOI of 10 for 0 ...
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bioRxiv - Immunology 2020Quote: ... the cleared lysate was mixed with the anti-FLAG M2 magnetic beads (SIGMA-ALDRICH) and rotated at 4 °C for 18 hours ...
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bioRxiv - Neuroscience 2020Quote: ... A mouse cytokine/chemokine magnetic bead panel (MCYTOMAG-70K-06; Millipore, Billerica, MA, USA) was employed to detect the levels of IFN-γ ...
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bioRxiv - Microbiology 2021Quote: Chromatin immunoprecipitation were done with 40 µl of Anti-FLAG M2 magnetic beads (Millipore) for 2h at 4°C under constant agitation ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitations were performed by adding 15 μL of magnetic FLAG beads (Sigma-Aldrich, M8823) to the lysates and rotating at 4°C for 2 hours before washing 3 times with a high salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... Other chemokines and cytokines were measured by the MILLIPLEX mouse magnetic bead assay (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... 1mM EDTA (binding buffer) and combined with M2 anti-FLAG magnetic beads (Sigma, M8823). Bound lysate was washed with binding buffer + 0.05% NP-40 ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysates were incubated with Anti-FLAG M2 Magnetic Beads (Sigma, Taufkirchen, Germany) end-over-end rocking for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Whole cell extracts were incubated with anti-FLAG M2 magnetic beads (Sigma-Aldrich, F7425) or GFP-Trap magnetic beads (Chromotek ...
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bioRxiv - Biochemistry 2021Quote: ... The cell lysate was incubated with anti-FLAG magnetic beads (Sigma-Aldrich, Milwaukee, WI). Beads were washed and myosin was eluted from the beads by 3x FLAG peptide (ApexBio ...
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bioRxiv - Molecular Biology 2021Quote: ... Serum TSH was analyzed with a mouse pituitary magnetic bead panel kit (EMD Millipore Corp. ...
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bioRxiv - Immunology 2022Quote: ... anti-MYC antibody (C3956) and anti-FLAG magnetic beads (10004D) were obtained from Sigma. Anti-FLAG antibody (rabbit source) ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was incubated with magnetic affinity beads carrying anti-FLAG (Sigma-Aldrich) or anti-GFP (Chromotek ...
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bioRxiv - Cancer Biology 2022Quote: FLAG Immunoprecipitation: 200 μL of 50:50 magnetic FLAG bead slurry (Millipore-Simga; M8823) were washed twice with Tris Buffer Saline (TBS ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate (8 mg/sample) was incubated with 200 µl anti-Flag magnetic beads (Sigma) at 4°C for 90 min ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were mixed with 40 μl of Anti-FLAG® M2 Magnetic Beads (Millipore) and incubated for 3 hours while rotating at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Sera-Mag magnetic bead mix (1:1 ratio of Sigma-Aldrich, GE45152105050250 and GE65152105050250) was added in a 25:1 protein to bead volume ratio and proteins were bound onto the beads by adding an equal volume (protein plus beads ...
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bioRxiv - Biophysics 2023Quote: ... The resulting supernatant was incubated overnight with anti-FLAG M2 magnetic beads (Sigma-Aldrich) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatants were incubated with 10 µl FLAG M2 Magnetic Beads (Milipore-Sigma) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich, St. Louis, MO). Bound proteins were eluted by addition of 500ug/mL FLAG peptide (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with pre-washed 50 μl of magnetic-streptavidin beads (MyOne C1, Sigma) for 1.5h in cold room rotating ...
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bioRxiv - Microbiology 2024Quote: ... Then 400 µg of cell lysate was added to anti-HA magnetic beads (Sigma) in the presence of RNaseA (50 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were then added to prewashed anti-FLAG M2 magnetic beads (Sigma, Cat. # M8823) or anti-ALFA ST magnetic beads (NanoTag Biotechnologies ...
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bioRxiv - Plant Biology 2020Quote: ... antibodies and Protein A agarose beads (Sigma). After elution of immune complexes ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A sepharose beads (Sigma-Aldrich, P3391). Neutravidin Resin (Piere ...
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bioRxiv - Plant Biology 2020Quote: ... bound to Protein-A agarose beads (Sigma) were used to immunoprecipitate the genomic DNA fragments ...