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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Mouse Protein DEK DEK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: The RIP assay was performed using a Magna RIPTM RNA Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: The RBD and spike proteins were chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). For the RBD ...
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bioRxiv - Cancer Biology 2020Quote: RIP assay was performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma, Burlington, MA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations of wholecell lysates were determined by the QuantiPro™ BCA Assay Kit (Sigma-Aldrich, QPBCA). The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... Interactions between CB1 and cytoskeleton proteins were detected using the Duolink in situ PLA detection Kit (Sigma) following the instructions of the supplier ...
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The ECM fraction was isolated from the frozen tissues using the Compartment Protein Extraction Kit (Millipore, #2145) as per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were extracted from residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Metabolite profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under the control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Microbiology 2023Quote: RNA-binding protein immunoprecipitation (RIP) was conducted using the Magna RIP kit (catalog no. 17-700; Millipore) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial and cytoplasmic protein was isolated using the mitochondrial/cytoplasmic fractionation kit from Millipore Sigma (cat.#: MIT1000). Primary antibodies used include the following ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-mouse agarose or mouse agarose (both Sigma) were added for 1 hour at 40C prior to 3 washes in Lysis Buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse anti-mouse NeuN (Millipore, MAB377, 1:100), and Rabbit Anti-MBP (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... and proteins precipitated using protein-A Agarose (Sigma-Aldrich) prior to analyses by Western blotting.
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bioRxiv - Microbiology 2022Quote: ... proteins proteins were electrophoretically transferred to nitrocellulose membranes (Millipore) using a Transblot semi-dry transfer cell (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... the protein was quantified by PierceTM BCA Protein Assay Kit (Thermo ScientificTM, Rockford, IL, USA) and the carbohydrate was quantified by Total Carbohydrate Assay Kit (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Molecular Biology 2021Quote: PLA was performed using DuoLink red detection kit and DuoLink anti-mouse and anti-rabbit antibodies (Millipore-Sigma Cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... and analysed using the WesternBreeze Chromogenic anti-mouse Kit with a primary anti-FLAG (F4042, Sigma-Aldrich) antibody at a 4000x dilution ...
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bioRxiv - Cell Biology 2021Quote: Duolink In Situ Red Starter Kit Mouse/Rabbit Proximity Ligation Assay was purchased from Millipore Sigma (DUO92101) and was performed according to the manufactures protocol using KLK10 and HTRA1 antibodies described above.
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bioRxiv - Cell Biology 2019Quote: ... The proximity ligation assay was conducted using a DuoLink In Situ Mouse/Rabbit PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: Proximity ligation assay was performed using the Duolink® In Situ Orange Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: Cytokine quantification was determined using the MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Kit (MCYTMAG-70K-PX32, Millipore). The assay was conducted as per the manufacturer’s instructions and plates were ran on a MAGPIX instrument with xPONENT software ...
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bioRxiv - Molecular Biology 2023Quote: ... Proximity ligation was performed using the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Cell Biology 2021Quote: ... and screened for expression of the fusion proteins by western blotting with an anti-FLAG M2-HRP antibody (Sigma, mouse monoclonal, cat#: A8592).
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblotting for specific proteins was carried out using the following primary antibodies: Mouse monoclonal antibodies against anti-FLAG M2 (Millipore Sigma, Billerica, MA), Jak1 (CST-50996) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was detected using the Simple-Western-JESS capillary western blot system (Bio Techne) multiplexed for total protein and chemiluminescence detection of mouse αSFPQ (1:2000; Sigma-Aldrich WH0006421M2, RRID:AB_1843565) or mouse αTIAL1 (1:10 ...
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bioRxiv - Developmental Biology 2023Quote: ... Puromycin incorporated into the proteins were detected in a Western blot using a mouse-origin anti-puromycin monoclonal antibody (12D10, Sigma MABE343, lot # 3484967) and a goat-anti-mouse HRP-conjugated secondary antibody (Abcam ab6789 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Genetics 2020Quote: ... Antibody bound DNA-proteins were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed ...
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bioRxiv - Physiology 2019Quote: ... The silver stained protein spots were de-stained using the ProteoSilver Plus Stain Kit (Sigma-Aldrich, MO, USA), and in-gel enzymatic digestion was done overnight at 37°C using Trypsin Singles ...
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bioRxiv - Physiology 2019Quote: ... Gels containing protein spots were then stained using the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich, MO, USA) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The experiment was conducted with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore,17-700 Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: An RIP assay was performed using a Magna RIP RNA binding protein immunoprecipitation kit (17-700; Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RIP assay and RNA pull down assay were performed using RIP RNA-Binding Protein Immunoprecipitation kit (Sigma, USA) and Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The extraction of nuclear proteins has been performed using the CelLytic™ NuCLEAR™ Extraction kit (Sigma-Aldrich) complemented with PIC ...
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bioRxiv - Physiology 2020Quote: ... Subcellular fractions were extracted using differential lysis and solubilization with a commercial Millipore Compartmental Protein Extraction Kit (Millipore) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Lactate and pyruvate were measured in the protein-depleted flow-through using assay kits (MAK064-1KT, Sigma-Aldrich) and (ab65342 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant and standards containing bovine serum albumin (BSA) were analyzed using a BCA protein assay kit (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Lipid profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein quantification for each sample was conducted by BCA Assay in accordance with kit instructions (Millipore Sigma 71285M).
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bioRxiv - Developmental Biology 2021Quote: IGF1 levels were measured in serum and SSPC culture supernatant using ELISA (Sigma-Aldrich, RAB-0229) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... as well as BG505 Env-NP expression by ELISA (NP capture: Galanthus nivalis lectin (GNL, Sigma) / NP detection ...