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Citations for Millipore Sigma :
651 - 700 of 1716 citations for Mayaro virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor cell lines and UPCs were infected with the concentrated virus in a culture media containing 8 µg/ml Polybrene (Sigma) and positively transduced cells selected by FACS for the expression of the corresponding fluorescent reporter.
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bioRxiv - Biophysics 2023Quote: ... The cells were then infected in triplicate with VSV-G-pseudotyped GFP-encoding virus in the presence of polybrene (10 µg/ml; Sigma) at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... The next day 50 µl of concentrated virus was added together with 5 µg/ml polybrene (Millipore, TR-1003-G) to the culture media ...
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bioRxiv - Immunology 2024Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/mL of cytosine arabinoside (Sigma-Aldrich), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Biophysics 2020Quote: ... Cell lysate was incubated with anti-FLAG magnetic beads (Sigma-Aldrich, Milwaukee, WI). Beads were washed and myosin was eluted from the beads by 3x FLAG peptide (ApexBio ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were isolated and added 50 µL of glutathione agarose beads (Sigma, G4705). The samples were incubated overnight and captured ubiquitinated protein was eluted in SDS reducing sample buffer.
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bioRxiv - Cancer Biology 2021Quote: ... Whole- cell lysates were incubated with anti-FLAG M2 agarose (Sigma A2220-10ML) or anti-HA agarose (Sigma E6779-1ML) ...
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bioRxiv - Immunology 2021Quote: ... The lysates were incubated with 1.5 μl of monoclonal Anti-Flag M2 (Sigma) for 2 hrs at 4 °C with gentle agitation followed by the addition of 25 μl of Protein A/G magnetic beads (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... and the resulting lysates incubated with 30 µl anti-FLAG M2 (Sigma, A2220) pre-conjugated agarose-resin for 2-3 hours at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 50 mL lysate was amended with 5g PEG8000 and 3.3 g NaCl (Sigma), shaken until dissolved and incubated on ice overnight ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lysates and concentrated particles were analyzed for FLAG (Sigma–Aldrich; A8592; 1:10,000), GAPDH (Sigma–Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... lysates were collected on-plate with 1x RIPA buffer (EMD Millipore #20-188) with protease and phosphatase inhibitors (ThermoScientific #1861280) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 µg lysate was treated with 0.5 µl alkaline phosphatase (Sigma Aldrich, P0114) and incubated at 37 °C for 2 h ...
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bioRxiv - Microbiology 2019Quote: ... One tube of lysate was treated with 1 μL of trehalase (Sigma-Aldrich) enzyme or vehicle control ...
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bioRxiv - Biochemistry 2019Quote: ... and the resulting lysate was immunoprecipitated using anti-FLAG-conjugated agarose beads (Sigma) in a 50 mM Tris buffer ...
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bioRxiv - Immunology 2020Quote: Protein lysates were subjected to SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked and probed with one of the following antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were subjected to SDS PAGE electrophoresis and transferred onto PDVF membrane (Millipore). Membranes were probed with antibodies against YAP (Cell Signaling Technology 4912) ...
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bioRxiv - Cancer Biology 2021Quote: ... cell lysates were incubated with anti-HA affinity-agarose beads (Sigma, cat. E6679) at 4 °C overnight on a shaking platform ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were incubated with 50 μL of Myc-tag beads (A7470, Sigma-Aldrich, blocked with 5% BSA for one hour at room temperature) ...
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bioRxiv - Biochemistry 2020Quote: ... the clarified lysates were mixed with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours at 4 °C on a rotator ...
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bioRxiv - Bioengineering 2021Quote: ... Whole cell lysates were normalized to HRP-tagged Actin antibody (#A3854, Sigma Aldrich) and nuclear fractions to HRP-tagged Histone H3 (#12648 ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were loaded in 10% polyacrylamide gels and transferred to Immobilon membranes (Millipore) for Western blot ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and lysates were prepared in RIPA buffer (cat #R0278, Sigma) supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were prepared in RIPA buffer (containing protease (1:100; Sigma, P8340) and phosphatase inhibitors (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... the cleared lysate was bound with 50µl of FLAG-M2 resin (Sigma, A2220) at 4°C for 2 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were subjected to SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% nonfat milk for 1 h at room temperature and then probed with the indicated primary antibodies at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Bacteriophage lysate was concentrated using an Amico® Ultra-15 centrifugal filter (Millipore) and LB broth was replaced with a solution of 10 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: ... Cleared lysates were incubated with 10μL of anti-FLAG M2 affinity beads (Sigma) for 2 hours at 4°C with rotation ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: Cell lysates were prepared on ice in RIPA buffer (Sigma-Aldrich, cat. R0278) with freshly added cocktail of proteases (Millipore-Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cleared cell lysate was filter with 0.45 µm sterile filter (Millipore Merck, Schwalbach) and loaded onto a 5 ml HiTrap Chelating HP column (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The lysates were further incubated with 125 units of Benzonase (E1014-25KU, Sigma) in an end-over-end rotator at 4 °C for 20 min ...
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bioRxiv - Cell Biology 2021Quote: The keratinocyte lysates were prepared in RIPA buffer with protease inhibitors (Sigma, #P2714) and sonicated at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 2mg of cleared lysates were incubated with 3μg anti-Flag antibody (Sigma F3165) for 2 hours at 4°C with constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were resolved by SDS-PAGE and transferred onto PVDF membranes (Millipore). Membranes were blocked with 2% BSA in TBS tween20 0.1% and incubated in the presence of the primary and then secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... whole cell Triton X-100 lysates were incubated with Flag-M2 agarose (Sigma) and washed with lysis buffer three times ...
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bioRxiv - Biochemistry 2022Quote: ... clarified lysate was incubated with 20 μl Anti-FLAG M2 paramagnetic beads (Sigma) per ml of lysate for 2 h to overnight rotating at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... IP was carried out by incubating lysates with OGT antibody (Sigma-Aldrich O6264), OGlcNAc antibody (CTD 110.6 ...
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bioRxiv - Genetics 2022Quote: ... The cell lysates were incubated with anti-Flag M2 affinity beads (Sigma, F2426) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cell lysates were incubated with antibody and protein A/G magnetic beads (Sigma) from 3 h to overnight at 4°C under rotation conditions ...
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bioRxiv - Neuroscience 2020Quote: ... Cleared lysate was incubated with anti-FLAG M2 magnetic beads (Sigma-Aldrich, M8823) overnight with rotation at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... lysates were incubated overnight with ANTI-FLAG® M2 Affinity Gel (Sigma-Aldrich), EZview™ Red Anti-HA Affinity Gel (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... Total cell lysates were prepared using RIPA buffer (Sigma-Aldrich, catalogue no. R0278), supplemented with 10 μL/ml protease inhibitors cocktail (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and the cell lysates were co-immunoprecipitated by anti-Flag agarose (Sigma, A2220), separated by SDS-PAGE and visualized by staining ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleared lysates were added to 20 µL anti-FLAG M2 magnetic bead (Sigma) solution (50% beads per total volume with Beads Buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Physiology 2019Quote: ... cell lysates were prepared in buffer K containing protease inhibitor cocktail (Sigma, #P2714). The cell lysates were centrifuged at 12,000g for 10 mins at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...