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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Virus and cell pellets were solubilized in lysis buffer [10mM iodoacetamide (Sigma-Aldrich), CompleteTM protease inhibitor tablets (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was aspirated and cells resuspended in virus medium containing TPCK-trypsin (Sigma), 20 mM HEPES (Corning ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Microbiology 2024Quote: ... The virus-containing supernatant was filtered through 0.45-mm polyvinylidene difluoride filters (Millipore) and precipitated in 8.5% polyethylene glycol [average molecular weight (Mn) ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus containing medium with 8 μg/mL polybrene (Sigma-Aldrich, Cat #H9268) was used in 1:1 dilution with the culture medium to infect SKBR3 and HCC1954 cells ...
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bioRxiv - Neuroscience 2023Quote: ... the virus-containing medium was filtered through Costar Spin-X columns (Sigma #8162) as previously described 31.
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bioRxiv - Neuroscience 2023Quote: ... The virus was slowly mixed with silk fibroin solution (50mg/mL, Millipore Sigma) with a ratio of 1:1 through the pipette to avoid generating bubbles ...
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bioRxiv - Immunology 2023Quote: ... virus particles were precipitated by adding 50% (w/v) PEG 6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... and Anti-Adeno-associated Virus 9 Antibody clone HL2374 (Millipore Sigma, #MABF2326-25UG). Following three additional washes ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Virus-containing medium was passed through a 0.45 µm filter (cat. #SLHVR33RB, Millipore), aliquoted and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... individuals performed a nasopharyngeal swab into virus transport medium (VTM; MWE Sigma-Virucult), gave details on any recent infection episodes ...
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bioRxiv - Microbiology 2021Quote: Viral stocks of molecular clones HIV-1NL4-3 and HIV-1NL4-3 BaL (AIDS Reagents Program) were generated by transfecting HEK293T cells using PEI transfection reagent (Sigma, Cat: 408727). Pseudotyped HIV-1VSVg-NL4-3 ...
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bioRxiv - Microbiology 2023Quote: ... J-Lat 10.6 and J-Lat 5A8 previously knocked out for ZAP (12) were transduced with HIV-CRISPR library lentivirus with DEAE-Dextran (final concentration 20 ug/mL, Sigma-Aldrich; D9885) at a multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2021Quote: ... nitrocellulose membranes were incubated with primary antibodies against α-E catenin (1: 1000; Cell Signalling Technologies, catalogue number # 240) and α-tubulin (1: 20 000; Sigma–Aldrich, catalogue number T6074) at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50), total SR proteins (1;1000 ...
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bioRxiv - Bioengineering 2019Quote: ... microtubule associated protein 2 (MAP2) (1:500, Sigma-Aldrich). The cells were subsequently washed 6 x 5 min each with PBS/0,1% Triton-X 100 ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-Glial Fibrillary Acidic Protein (1/1000, Millipore, MAB360). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced using 1 mM dithiothreitol (Sigma-Aldrich) for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Anti-ACTB reference protein (Sigma Aldrich, A2066, 1:10000) antibody was also used ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-microtubule associated protein 2 (MAP2, 1:200, Millipore), mouse monoclonal anti-ankyrinG (AnkG ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM NaVO3) with protein inhibitor cocktail (Sigma®) followed by total protein quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-SR proteins (Millipore, cat#MABE126, 1:2,000 dilution); anti-phosphoepitope SR proteins clone1H4 (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse antiglial fibrillary acidic protein (GFAP) (Millipore, 1:500), Beta-actin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... myelin basic protein (MBP; 1:200; MAB386; Milipore Sigma), Synaptophysin (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was expressed using a pETBlue-1 (Novagen) vector in E ...
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bioRxiv - Cell Biology 2024Quote: N-terminally His6-Smt3-tagged proteins (pRSFDuet-1, Novagen) were expressed in Rosetta (DE3 ...
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bioRxiv - Neuroscience 2023Quote: ... Thorough washing was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... cell monolayers were allowed to migrate for at least four hours before exchanging the medium with pre-warmed medium containing 10 μg/ml E-cadherin blocking antibody (clone DECMA-1, Millipore MABT26).
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bioRxiv - Immunology 2020Quote: ... 180 uL of co-culture media (RPMI-1640 media containing 1% FBS, LPS (100 ng/mL; from E coli, Sigma-Aldrich) and IFNɣ (100 ng/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... the broad-spectrum hydroxamate metalloproteinases inhibitor CT1746 (100 µM) and the protease inhibitor cocktail (a mixture of aprotinin, bestatin, E-64, leupeptin and pepstatin A, 1/1000)(SIGMA, P1860) with or without 20 nM Wnt5a in the presence or absence of 500 nM RAP for 3 h at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... All E. coli strains (a gift from Dr. Christian Rudolph) were routinely grown in modified Luria Broth (LB) (1% tryptone (Sigma-Aldrich), 0.5% yeast extract (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated with primary antibodies (CK8, Abcam ab182875,1:200. CK14, Biolegend 905301,1:400; E-cadherin, Biolegend 147301,1:200; α-SMA, Sigma A5228, 1:500) diluted in blocking buffer at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... CD11c+ were purified and either plated with no further supplementation or stimulated with 100 ng/mL or 1 μg/mL of lipopolysaccharide (LPS, E. coli O111:B4, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... 8-week old male and female Cx3cr1-GFP mice bred on a C57BL/6J background (n=2 females and 1 male/condition) were intraperitoneally injected with 0.5 mg/kg lipopolysaccharide (LPS; Lipopolysaccharides from E. coli O55:B5, Sigma-Aldrich L5418) or vehicle solution (PBS ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Genetics 2021Quote: ... were superovulated by serial Pregnant Mare Serum Gonadotropin (5 IU per mouse, Prospec Protein Specialists) and human chorionic gonadotropin (5 IU, Millipore) injections 46 h apart ...
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bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).