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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Ethyl 2 pyrrolidin 2 yl 2 3 dihydro 1 3 thiazole 4 carboxylate hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI; 1:1000; Sigma) for nuclei counterstaining ...
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bioRxiv - Bioengineering 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Sigma-Aldrich, USA), sodium hydroxide (Sinopharm Chemical Reagent Co. ...
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bioRxiv - Microbiology 2023Quote: ... 4′,6-diamidino-2-phenylindole (DAPI; 1:1000; Sigma-Aldrich) was used for nuclei staining and added to the secondary antibody solution ...
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bioRxiv - Developmental Biology 2023Quote: ... 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (1:1000; Sigma) was used as nuclear counterstaining ...
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bioRxiv - Developmental Biology 2022Quote: ... and 4′6-diamidino-2-phenylindole (1:5000, Sigma Aldrich). Images were acquired on a Leica DM5500 microscope equipped with a Leica DEC500 camera.
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bioRxiv - Cell Biology 2020Quote: ... and 1% of photoinitiator (2-hydroxy-2-methylpropiophenone - Sigma) was immediately introduced by capillary action between the PDMS stamp and the glass coverslip ...
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bioRxiv - Molecular Biology 2021Quote: ... MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide] dye solution (Sigma-Aldrich, USA) (5 mg MTT in 1 ml PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma-Aldrich) and phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich). Protein concentration was measured with BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... The coverslips were then immersed in 2% (v/v) (3-Aminopropyl)triethoxysilane (Sigma A3648) prepared in acetone for 2 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Cells were split every 2-3 days with trypsin (Sigma, Saint Louis, Missouri, USA) to maintain a subconfluent monolayer.
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bioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich) and phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich). Protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... concentrated to 2 ml using an Amicon concentrator with a 3 kDa cutoff (Millipore), and applied into a Superdex 75 (16/600 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 2× M63 buffer was made by adding 3 g KH2PO4 (Sigma-Aldrich cat. P5655), 7 g K2HPO4 (Sigma-Aldrich cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were subcultured every 2-3 days using trypsin (Sigma, Saint Louis, Missouri, USA) to maintain a subconfluent cell layer.
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bioRxiv - Biochemistry 2020Quote: Ebselen (2-Phenyl-1,2-benzisoselenazol-3(2H)-one) is commercially available from Sigma Aldrich.
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1X protease inhibitor and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich), for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 3 μM SHP1/2 phosphatase inhibitor (NSC-87877, Sigma-Aldrich) for 20 minutes prior to target particle addition ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed with 3 × 100 µL of 2% bovine serum albumin (BSA, Sigma) in D-PBS+CM and blocked in that same solution for 2 hours at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... 540-69-2] (pH 3, adjusted with formic acid [Sigma Aldrich, 64-18-6]) and solvent B was acetonitrile [Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... and 1.0% (v/v) phosphatase inhibitor cocktail #2 and #3 (Sigma-Aldrich P5726, P0044). Tissue homogenates were centrifuged at 14,000×g at 4°C for 20min and supernatants collected and stored at -80°C before initiating the protein assay.
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were transformed into pKM208 carrying Rosseta 2 (Millipore Sigma 71400-3) strain by electroporation ...
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bioRxiv - Developmental Biology 2022Quote: Myoblasts were transfected with either (final concentrations) 10 µM 2’,3’-cGAMP (Sigma Aldrich), 2 µg/mL poly(I:C ...
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bioRxiv - Microbiology 2022Quote: ... coverslips were incubated in a 2% solution of (3-Aminopropyl)triethoxysilane (APTES, Sigma-Aldrich) in acetone with agitation for two minutes ...
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bioRxiv - Bioengineering 2023Quote: The mice were anaesthetized with isoflurane (induction 5%, maintenance 2−3%; Sigma-Aldrich, USA) and secured in a stereotaxic instrument (RWD ...
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bioRxiv - Cell Biology 2022Quote: MCF10A cells (3×106 cells per condition) were fixed with 2% formaldehyde (Sigma, #252549) in PBS with 10% FBS for 10 min at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and Phosphatase Inhibitor Cocktails 2 and 3 (Sigma- Aldrich cat. P5726 and cat. P0044). Pellets were resuspended in lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μL each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044)) and lysate cleared by centrifugation at 17,000g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 2’-ODibutyryladenosine 3’,5’-cyclic monophosphate sodium salt (cAMP; 50 μM, Millipore Sigma, D0627), and cis-4 ...
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bioRxiv - Plant Biology 2024Quote: ... [3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine sulfonate] FerroZine (FZ) (Sigma-Aldrich, Bangalore, India) was added to create Fe-deficient conditions ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed at room temperature for 2 hours with 3% BSA (Sigma-Aldrich) PBS-Tween ...
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bioRxiv - Developmental Biology 2022Quote: ... 3-dpf larvae were anesthetized with 0.016 % Tricaine/Ethyl 3-aminobenzoate methanesulfonate salt (A5040, SIGMA-ALDRICH) and microinjected with 5-8 nL of liposome encapsulated clodronate (SKU# CLD-8909 ...
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bioRxiv - Neuroscience 2022Quote: ... Ethyl 3-aminobenzoate methanesulfonate (MS-222, Sigma; n = 10 larvae) was gradually added to the chamber water to achieve a concentration at which spontaneous eye movements stopped ...
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bioRxiv - Immunology 2022Quote: ... and 3-amino-9-ethyl-carbazol (AEC) substrate (Sigma Aldrich) diluted in N,N-Dimethylformamide (DMF ...
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bioRxiv - Immunology 2022Quote: ... spleens were fixed for 2 h at 4°C in 2 mL of 4% paraformaldehyde (Millipore Sigma) followed by an overnight incubation at 4°C in 2 mL of 30% sucrose (Millipore Sigma) ...
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bioRxiv - Zoology 2020Quote: ... 3-methyl-but-2-enyl acetate and (2E)-hex-2-enyl acetate were confirmed with analytical standards from Sigma-Aldrich (St. Louis, MO). For the analysis of CHCs ...
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Item no. 20515). Ammonium perfluoro(2-methyl-3-oxahexanoate) (GenX, CAS# 62037-80-3, Item no. C23990) was obtained from Sigma-Aldrich. PFOS came as a solution in 100% ethanol ...
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bioRxiv - Bioengineering 2020Quote: ... nalidixic acid (NA; N8878, BCBW6556), and 4′,6-diamidino-2-phenylindole (DAPI; D9542, 28718-90-3) were purchased from Sigma Aldrich, USA ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were surface sterilized and kept for stratification in dark at 4 °C for 2-3 days on half strength MS-agar (Millipore Sigma) plates ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting EZH2 (shEZH2#3 [TRCN0000040076] and shEZH2#4 [TRCN0000010475]) and FAK (shFAK#2 [TRCN0000121211], shFAK#3 [TRCN0000121318], and shFAK#21 [TRCN0000121321]) were purchased from Sigma-Aldrich. pRK5-TGFβRI-FLAG plasmid (#14833 ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT, 0.5 mg/ml in PBS, Sigma-Aldrich, USA) with 1 μM of menadione (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2024Quote: ... 1% Triton X-100] and incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Immunology 2024Quote: ... a subset of the mice (n = 3-4 per group) received intraperitoneal (i.p.) injections of 2 mg/kg WFA (Millipore-Sigma, #681535), or equivalent volume of the vehicle (VC ...
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bioRxiv - Bioengineering 2022Quote: ... 3 the cell culture media was replaced 1-2 hours before imaging with RPMI media (Sigma-Aldric R8755) supplemented with B27 ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately1-2 μL of endotoxin-free DNA (1-3 mg/ml) diluted in PBS/0.025% Fast Green (SIGMA) was injected into the lateral ventricles of the forebrain using heat-pulled glass micropipettes (Drummond) ...