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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for ERK1 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μM CHIR99021 (Cayman) and 1,000 U/ml recombinant mouse LIF (Millipore)39 ...
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bioRxiv - Microbiology 2021Quote: ... and 40 ng/mL bFGF (basic recombinant human fibroblast growth factor; Sigma) in flasks coated first with 50 µg/mL poly-L-ornithine (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and supplied with 1000 U/ml recombinant leukemia inhibitory factor (LIF, Millipore). 293T cells were cultured in DMEM supplemented with 10% FCS.
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bioRxiv - Biochemistry 2021Quote: ... and supplied with 1000 U/ml recombinant leukemia inhibitory factor (LIF, Millipore). ESCs were cultured on plates which were pre-coated with 0.1% gelatin ...
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bioRxiv - Genetics 2020Quote: ... 10 ng/μL recombinant fibroblast growth factor basic (βFGF; Sigma-Aldrich, #F0291), 10 μg/μL Heparin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 40 ng/ml recombinant human long-range IGF-1 (Sigma-Aldrich, USA), 5 ng/ml recombinant IL-6/soluble IL-6R fusion protein ((Fischer et al. ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 1000 units/ml ESGRO Recombinant Mouse LIF protein (Millipore #ESG1107), 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: Limited proteolysis experiments were conducted with recombinant human cathepsin S (Milipore-Sigma) diluted in phosphate-citrate buffer at pH 5.6 and 37 °C(21) ...
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bioRxiv - Synthetic Biology 2021Quote: Recombinant hACE2 with a C-terminal deca-histidine tag (SAE0064, Sigma-Aldrich) was resuspended in PBS to a final concentration of 50 ng/μL ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were digested overnight at 37 °C with recombinant trypsin (Trypzean, Sigma) (enzyme ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human holo-lactoferrin (iron-saturated) (L1294) was purchased from Sigma-Aldrich. Aliquots were prepared by resuspending in PBS at a concentration of 1 mg/ml and were stored at −20 °C ...
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bioRxiv - Immunology 2021Quote: ... Recombinant Wnt5A (Cat no.- 10UG 645-WN-010 R & D, GF146 Millipore) and recombinant Wnt3A (Cat no.- 10UG 5036-WN-010 R & D ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... eluted in water and aliquoted for later use with recombinant Cas9 (Sigma). Oligos used for sgRNA production are listed in Extended Data Table 4.
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... media supplemented with 1000U/mL ESGRO Recombinant Mouse LIF (Millipore Sigma, ESG1107), 2 mM GlutaMAX (Gibco ...
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bioRxiv - Immunology 2023Quote: ... then tested against recombinant proteins coated in Dulbecco’s PBS (DPBS, Sigma-Aldrich) overnight onto 96-well Immunlon-2 plates (VWR Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore).
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was digested by adding 10 U DNase I recombinant (Sigma) and incubating for one additional hour ...
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bioRxiv - Biochemistry 2023Quote: ... They were incubated with recombinant ULK1 proteins (Sigma, #SRP5096-10UG, Darmstadt, Germany) in the kinase buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 10 μg/μl recombinant mouse leukemia inhibitory factor (LIF; Sigma-Aldrich) in a 5% CO2 incubator at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG ...
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bioRxiv - Neuroscience 2019Quote: ... blocked with 0.5% bovine serum albumin and 0.2% triton X-100 for 2 hours and incubated with primary antibody (rabbit anti-Tyrosine hydroxylase, Millipore) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The following primary antibodies were used: monoclonal rabbit antibody against COX-2 (Clone SP21, Cat# SAB5500087-100UL, Sigma-Aldrich), mouse monoclonal anti-acetylated α-Tubulin antibody (Cat# sc-23950 ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS plus 0.1% triton (PBST) for 2 hours and then transferred to 1:1000 TH anti-rabbit primary antibody (Millipore) with 1% NDS in PBST overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The lysates were then subjected to Western blot with anti-FLAG M2 mAb (F3165, Sigma, 1:3,000), anti-HA mAb (HA.C5 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Immunology 2022Quote: ... The blots were subsequently reacted with mouse MAb AC-15 anti-β-actin (Sigma, St, Louis, MO) (1:200,000 ...
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bioRxiv - Genetics 2019Quote: ... transferred to nitrocellulose membrane and FLAG-Rad53 was detected by western blot with FLAG M2 mAb (Sigma).
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bioRxiv - Immunology 2021Quote: ... conv CD4+ cells were activated with 1μg/ml of soluble anti-human CD3 /OKT3 mAb (Sigma, USA) in the presence of irradiated allogenic PBMC (∼50,000 ...
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bioRxiv - Neuroscience 2023Quote: ... NBP1-92693AF647) and the oligodendrocyte transcription factor OLIG2 (Anti-OLIG2 clone 211F1.1 mouse mAb, Merck Millipore, MABN50A4). Samples were kept on ice throughout the isolation and staining procedure ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... sections were incubated in 4°C overnight with primary antibodies directed against PSD-95 (Millipore, MAB 1598). Next ...
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bioRxiv - Neuroscience 2023Quote: ... a mAb against Acetylated α-tubulin (clone 6-11B-1; Sigma-Aldrich, Cat. Number: T7451, RRID: AB_609894) diluted 1:1000 or 1:200 for STED microscopy and a rabbit polyclonal antibody against detyrosinated (Glu ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Cancer Biology 2024Quote: ... (2) 2% Alconox (Sigma 242985 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... the brain slices were blocked with the blocking solution (10% normal goat serum and 0.7% Triton X-100 in PBS) for 2-h at room temperature and subsequently incubated with primary antibodies (rabbit anti-Glutamate Sigma, #G6642 ...
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bioRxiv - Neuroscience 2020Quote: ... blocked in normal donkey serum in PBST (2%) and transferred to rabbit anti-Fos primary antibody (1:10000; EMD Millipore) in PBST and normal donkey serum for 16 hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Flag (M2) and anti-MAP2 (HM-2) antibodies as well as polyclonal rabbit anti-Flag antibodies (F3165, F7425) were from Sigma. Anti-Xpress antibodies were from Invitrogen (46-0528).
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bioRxiv - Microbiology 2020Quote: ... incubated for 2 h with horseradish peroxidase (HRP)-conjugated secondary antibody (12-341 Rabbit anti-chicken RAC-HRP, Sigma-Aldrich) diluted 1:15000 in BSA-TBST ...
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bioRxiv - Microbiology 2020Quote: ... In this case the blot was incubated with the IcdH primary antibody at room temperature for 2 hours and with the secondary antibody (anti-rabbit HRP, Sigma) was done at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Physiology 2019Quote: ... fiber type IIb) (hybridoma supernates, Developmental Studies Hybridoma Bank) supplemented with rabbit polyclonal anti-laminin antibody (2 μg/ml, Sigma) overnight at 4°C ...