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Citations for Millipore Sigma :
651 - 700 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (Millipore) and immunoblotted with the indicated antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... and biotinylated-Hyaluronan Binding Protein (Millipore, 1:50) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred to nitrocellulose (Sigma #10600001) using a wet transfer apparatus (Bio-rad #1704070) ...
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bioRxiv - Cancer Biology 2024Quote: ... Parallel protein A/G beads (EMD Millipore, #IP0515ML), H3K4Me (Cell Signalling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were wet transferred onto PVDF membrane (Millipore) for 1 h at 100 V ...
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bioRxiv - Neuroscience 2024Quote: Proteins were reduced (10 mM DTT, EMD Millipore) and alkylated (30 mM iodoacetamide ...
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bioRxiv - Cancer Biology 2024Quote: ... 50μg protein emulsified with complete Freund’s adjuvant (Sigma) was subcutaneously (s.c. ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% protein inhibitor cocktail (Sigma- Aldrich: P8849)] ...
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bioRxiv - Cell Biology 2024Quote: ... and proteins alkylated with 55 mM iodoacetamide (Sigma) in 100 mM NH4HCO3 for 20 mins at RT (protected from light) ...
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bioRxiv - Neuroscience 2024Quote: ... using BCA Protein Assay (Sigma-Aldrich, CA, USA). For all calculations ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a PVDF membrane (Millipore). Membranes were blocked with 5% (wt/vol ...
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bioRxiv - Neuroscience 2024Quote: Protein was extracted in RIPA buffer (R0278, Sigma) supplemented with protease inhibitor cocktail (P8340 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto polyvinylidene fluoride membrane (Millipore). Membranes were blocked with 10% non-fat milk in tris-buffered saline containing 0.3% Tween 20 (TBST ...
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bioRxiv - Systems Biology 2024Quote: ... The proteins were reduced with TCEP (Sigma-Aldrich), final concentration 5 mM for 30 min at 37 °C and alkylated with 10 mM IAA (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1% protein inhibitor cocktail (100×) (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was quantified using BCA1 from Sigma. Thirty micrograms of protein lysate were separated by 6% Bis-Tris Gel ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2021Quote: CDK5 was immunoprecipitated from cells samples at different circadian times (CTs) (circa 200 μg of protein extracts. After immunoprecipitation at 4°C overnight with 2x Protein A agarose (Sigma-Aldrich), samples were diluted in washing buffer and split in two halves ...
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: Protein adsorption to the films was analyzed by incubation in a bovine serum albumin-FITC protein solution (1mg/ml, Sigma Aldrich) overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Microbiology 2021Quote: ... were immunized in a prime-boost-boost regimen with post-fusion hMPV B2 F protein (50 µg protein/mouse) in a water in oil emulsion with Titermax Gold adjuvant (Sigma-Aldrich) via subcutaneous (SC ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Protein content was measured by the BSA (cells) or microBSA (EVs) protein assay according to the manufacturer’s instructions (Sigma Aldrich, USA).
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
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bioRxiv - Systems Biology 2021Quote: ... the gel was scanned for total protein quantification and the proteins were subsequently transferred to an Immobilon-FL PVDF membrane (CAT # IPF00010, EMD Millipore). The membrane was probed with Rabbit anti-RAN (CAT # PA 1-5783 ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: Native PAGE-separated proteins or SDS PAGE-separated proteins were then transferred to PVDF membranes (EMD Millipore, catalog #IPVH00010, lot #R1EB02212) and subjected to Western blotting analysis as described previously (Li et al ...
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bioRxiv - Plant Biology 2023Quote: ... then proteins were extracted by adding 5 volumes of protein extraction buffer containing 1X MS-SAFE protease and phosphatase inhibitor (Sigma MSSAFE) and 1mM PMSF ...
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bioRxiv - Microbiology 2024Quote: ... followed by protein G coupled magnetic beads at room temperature for 10 minutes (PureProteome Protein G Magnetic Bead System, Millipore LSKMAGG). The beads were removed using a magnetic rack ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The obtained protein lysates were pre-cleared by centrifugation and incubated with 100 μl (50% slurry) of protein A beads (Millipore Sigma), coated specific antibodies or corresponding species-specific IgG isotype control in 1 ml of 1 × IP buffer ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: Antigen functional activity was confirmed by assessing ACE2 binding relative to a spike protein positive control (Callaghan Innovation, New Zealand) and ovalbumin negative control protein (Sigma, #A5503). ELISA plates were coated for 1 hour by incubation at ambient temperature with 50 μL antigen (0.072 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Cell Biology 2024Quote: ... Pelleted debris was discarded and supernatants (solubilized mitochondrial proteins or protein complexes) were transferred to fresh tubes and coomasie brilliant blue G (CBBG, Sigma B0770) was added from a 5% stock to give a 4 g:g (digitonin:CBBG ...
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bioRxiv - Microbiology 2024Quote: ... at low voltage (50 V) and constant current in a 4°C until the protein marker (BLUeye Prestained Protein Ladder, Sigma-Aldrich) front reached the end of the gel ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and precleared using protein A agarose beads (25%, diluted in ChIP dilution buffer; Protein A Agarose/Salmon Sperm DNA, Millipore, 16-157) and incubated overnight at 4°C (final buffer composition during antibody incubation was 0.1% SDS ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were performed on both total proteins and proteins eluted from the anti-GFP affinity beads using anti-FLAG (Sigma-Aldrich, F31651), anti-GFP (Abcam ...
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bioRxiv - Microbiology 2024Quote: Approximately equal amounts of GST fusion proteins and alanine-mutated GST fusion proteins were separated by 12% SDS-PAGE and transferred to PVDF transfer membranes (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk powder for 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto an immobilon-FL membrane (Millipore) at 75 V for 1hr ...
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bioRxiv - Physiology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merk Millipore), which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST ...