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Citations for Millipore Sigma :
651 - 700 of 6722 citations for Borrelia burgdorferi sensu stricto B31 ErpD Arp37 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... followed by protein G coupled magnetic beads at room temperature for 10 minutes (PureProteome Protein G Magnetic Bead System, Millipore LSKMAGG). The beads were removed using a magnetic rack ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The obtained protein lysates were pre-cleared by centrifugation and incubated with 100 μl (50% slurry) of protein A beads (Millipore Sigma), coated specific antibodies or corresponding species-specific IgG isotype control in 1 ml of 1 × IP buffer ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and precleared using protein A agarose beads (25%, diluted in ChIP dilution buffer; Protein A Agarose/Salmon Sperm DNA, Millipore, 16-157) and incubated overnight at 4°C (final buffer composition during antibody incubation was 0.1% SDS ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were performed on both total proteins and proteins eluted from the anti-GFP affinity beads using anti-FLAG (Sigma-Aldrich, F31651), anti-GFP (Abcam ...
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bioRxiv - Microbiology 2024Quote: Approximately equal amounts of GST fusion proteins and alanine-mutated GST fusion proteins were separated by 12% SDS-PAGE and transferred to PVDF transfer membranes (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk powder for 2 hours at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto an immobilon-FL membrane (Millipore) at 75 V for 1hr ...
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bioRxiv - Physiology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merk Millipore), which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST ...
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bioRxiv - Biophysics 2021Quote: ... FnCas9 protein was purchased from Millipore Sigma (FNCAS9PROT-250UG).
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were expressed in Rosetta 2 cells (EMD Millipore) grown in LB at 37°C and induced at an OD600 of 0.7 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were wet transferred onto PVDF membrane (Millipore, IPFL00010), blocked with 5% skimmed milk and probed successively with anti-Flag (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... beads containing bound proteins were incubated with thrombin (Novagen) overnight at 20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to Immobilon-FL PVDF membrane (Millipore), and membranes were incubated in Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration in S3 was normalized via Bradford (SIGMA) to ensure that equal amount of protein was loaded before ultracentrifugation step ...
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bioRxiv - Molecular Biology 2022Quote: ... protein complex were eluted with 10mM Maltose (Sigma Aldrich). Resolve elutes by SDS-PAGE and transfer to PVDF membrane for western blots ...
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bioRxiv - Synthetic Biology 2022Quote: ... The protein sample was transferred to PVDF membranes (Millipore) using Mini Trans-Blot (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein estimation was performed using Bradford assay reagent (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... Antibodies were bound to protein G-Sepharose (Sigma-Aldrich) in RSB-100 buffer containing 0.1% NP40 ...
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bioRxiv - Molecular Biology 2021Quote: Protein mixtures were Trichloroacetic acid (Sigma Aldrich, Cat# T0699) - precipitated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... protein bands were visualized with Ponceau stain (Sigma, P7170) to confirm protein transfer and even loading.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were transferred to nitrocellulose membranes (Sigma #GE10600002). Western blot analysis was performed under standard conditions with antibodies against Tubulin (Sigma #T9026 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred onto Immobilon-FL PVDF membranes (Millipore) and membranes were blotted with Anti-Flag M2 antibody (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... proteins were transferred to Nitrocellulose membranes (GE10600012, Sigma-Aldrich). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... 75 μl pre-washed protein A sepharose (Sigma-Aldrich) was added for recovery of FLAG-antibody and incubated for 30 min ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Merck Millipore) for 1h at 20V ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G Sepharose (Millipore Sigma Cat. GE17-0618-01) preblocked in 5% BSA was added and incubated for 1 additional hour rotating at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein concentration was determined using Bradford reagent (Sigma-Aldrich). Total protein extracts (30 μg ...
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bioRxiv - Cancer Biology 2019Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50), total SR proteins (1;1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein concentration was determined using Direct Detect spectrometer (Millipore). 20 µg of protein per sample was denatured at 70°C for 10 mins in 1× LDS loading buffer ...
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bioRxiv - Bioengineering 2020Quote: ... After concentrating the protein solution with Centriprep concentrators (Millipore), the final working concentration of protein was found to be 100 μg/ml using a BCA Protein Assay kit (Thermo Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were eluted off by stripping buffer (Millipore 2504) and re-probed for total Cx43 using the Sigma anti-rabbit antibody ...
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bioRxiv - Plant Biology 2019Quote: ... the protein concentration was estimated by Bradford (Sigma-Aldrich) and SDS-PAGE analysis (Supplementary Figure S1).
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bioRxiv - Biochemistry 2019Quote: ... Protein was then estimated using Bradford’s reagent (Sigma, USA).
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein emulsion was made in complete Freund’s adjuvant (Sigma) for subcutaneous immunization in two rabbits followed by three successive boosters in incomplete adjuvant at four week intervals.
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bioRxiv - Biophysics 2019Quote: ... Protein estimation was done using Bradford reagent (Sigma Inc.) and stored in −80°C after flash freezing in liquid nitrogen.
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bioRxiv - Biochemistry 2019Quote: ... The supernatants were passed through a Protein A (Sigma) affinity column ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) followed by incubation in Odyssey blocking buffer (LI-COR ...
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bioRxiv - Plant Biology 2019Quote: ... After incubation with Protein A agarose beads (Millipore, USA), enriched DNA fragments used as the templates for PCR were eluted out and purified with phenol/chloroform (1:1 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein G Sepharose 4 Fast Flow (P3296 Sigma-Aldrich) were incubated with 2 µg of GFP antibody raised in Goat for 2h at 4°C and were then washed with lysis buffer ...
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bioRxiv - Plant Biology 2019Quote: ... and Protein G magnetic beads (EMD Millipore, Burlington MA) pre-treated with 0.1% (w/v ...
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bioRxiv - Immunology 2019Quote: ... and 20 μl of Magna protein A beads (Millipore). Bound Saa3 promoter sequences were quantified using SYBR Green-based real-time PCR (target sequence and primers listed in Table S4) ...