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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... After rinsing sections were incubated 10 min at room temperature (RT) with 0.001 % DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, Sigma, D9542) in PBS for nuclear staining ...
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bioRxiv - Immunology 2022Quote: ... The sections were washed in PBS and incubated 10 min with 4’,6-diamidino-2-phenylindole (DAPI, 1/10,000, Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed with 3% PFA/PBS and the nuclei stained with 4’,6-diamidine-2’-phenylindole (DAPI, Sigma). An inverted fluorescence microscope (Zeiss ...
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bioRxiv - Developmental Biology 2020Quote: ... An incubation was done 45 min at room temperature with a mix of 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, 1µg/mL, 10236276001, Sigma-Aldrich) and the secondary antibody Donkey anti-Mouse Alexa Fluor 555 in PBS 1X ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Biochemistry 2022Quote: ... cells were mounted in Sigma-Aldrich DuoLink in situ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich), allowed to incubate at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... quenched with 25 mM ammonium chloride in PBS for 10 min and nuclei were stained with 4’,6-diamidino-2-phenylindol (DAPI, Sigma, cat ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 30min at room temperature (10µg/mL, D9542, Sigma-Aldrich). Coverslips were mounted with ProLong Gold Antifade (P36934 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... slides were washed for 5 min in 0.1x SSC with 1% Triton X-100 at 62°C and counterstained with 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in antifade with DABCO (1,4-diazabicyclo (2.2.2)-octane ...
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Immunology 2022Quote: ... The sections were then washed with PBS/0.5% Triton and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Digitally scanned sections were analysed using Image Scope software (Scanscope XT ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
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bioRxiv - Developmental Biology 2022Quote: ... Samples were also washed for 10 minutes in a solution of 1.25×10−3 mg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Sigma: D9542) in PBS to fluorescently label nuclei within tissue sections.
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bioRxiv - Immunology 2023Quote: 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) was used to exclude dead cells and debris (Sigma Aldrich, St. Louis, MO). All antibodies are from Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS and the cells’ nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL, Sigma-Aldrich) for 5 min at room temperature in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... the respective secondary antibodies were incubated in ADB at room temperature in the dark for 90 min together with 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich). Before imaging the cells were washed again using PBS ...
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bioRxiv - Cell Biology 2024Quote: ... samples were washed in 1X PBS and incubated with DAPI (4-6-diamidino-2-phenylindole, Sigma-Aldrich, 1 µg/mL) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... coverslips were incubated for 1 h in blocking medium containing the appropriate secondary antibody and 0.1 μg 4’,6-diamidine-2’-phenylindole (DAPI; Sigma-Aldrich). After three washes with PBS-T plus one additional washing step with PBS ...
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bioRxiv - Microbiology 2024Quote: ... in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst or 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI)) or ExtrAvidinCy3 (Sigma) solution (1:100 in 0.1% BSA/PBS supplemented with 5 μg/ml Hoechst) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS and further incubated for 15 min at room temperature with DAPI (4’, 6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibody lasted for 3 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent phalloidin was used to stain filamentous actin and DAPI (4′,6-diamidino-2-phenylindole) was used to stain nuclei (Sigma). Of note ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1x 30 min with 5×SSCT containing 4′,6-diamidino-2-phenylindole (DAPI, 1μg/ml, #62248, ThermoFischer) and/or Phalloidin Atto 565 (1:50, #94072, Sigma Aldrich), and 1x 5min in 5xSCCT ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were washed in PBS and further incubated for 15 min at room temperature with DAPI (4’,6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibody lasted for 3 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT, 0.5 mg/ml in PBS, Sigma-Aldrich, USA) with 1 μM of menadione (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-ura / SC-ura + 50µg/ml 6-azauracil (6-AU, Sigma), SC-leu / SC-leu + 0.4mM CuSO4 (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 6-hydoxydopamine (6-OHDA; cat. #H4381; Sigma-Aldrich, St. Louis, MO) was dissolved in vehicle solution (0.1 % ascorbic acid [cat ...
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bioRxiv - Physiology 2021Quote: ... and IL-6 (KIT ELISA IL-6-RAB0308-1KT, Sigma-Aldrich) in BALF following the kit instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were selected with 6 µg/mL 6-thioguanine (Sigma-Aldrich). The generated mutant cells were stocked in CELLBANKER 1 (Nippon Zenyaku Kogyo).
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bioRxiv - Neuroscience 2024Quote: For the intranasal administration of 6-hydroxydopamine (6-OHDA; Sigma-Aldrich), MMTV-PyMT +/-female mice at 1 week after the tumor onset were briefly anesthetized with 3% isoflurane ...
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bioRxiv - Biophysics 2023Quote: ... Both resiniferatoxin (Alomone Labs) and 6’-iodoresiniferatoxin (6’-iRTX, Sigma-Aldrich) were dissolved in ethanol to create a 1 mM stock and subsequently diluted to a 200 nM working concentration with the bath solution ...
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bioRxiv - Microbiology 2022Quote: ... The forward primer contained a 6-FAM (6-carboxyfluorescein) tag (Sigma). EMSA reaction mixtures (25 μL volume ...
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bioRxiv - Biochemistry 2020Quote: ... 7-amido-4-methyl-coumarin (NHMec) from the commercially available substrate Leucine-7-amido-4-methyl-coumarin (Sigma-Aldrich). For processivity assays ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... HEK293T cells were seeded in a 6-well plate so that they were 70-90% confluent the next day (∼0.8x10^6 - 1x10^6 cells/well in 2 mL of standard growth media containing 10% FBS (Millipore, TMS-013-B) and 1x Penicillin-Streptomycin (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... 0.003% 1-phenyl-2- thiourea (Sigma, St Louis, MO, P7629) was added to EM from 20-24 h postfertilization to prevent pigment formation ...
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bioRxiv - Developmental Biology 2022Quote: ... and 0.03mg/ml 1-phenyl-2-thiourea (P-7629; Sigma)] ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1 mM 1-phenyl-2-thiourea (PTU; Sigma-Aldrich, P7629) has been added to fish water from 1 dpf to prevent melanogenesis ...
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bioRxiv - Bioengineering 2024Quote: ... 0.0025% 1-phenyl 2-thiourea (Sigma-Aldrich, St-Louis, MO) was introduced to E3 embryonic medium between 20-24 hours post fertilization [22,23] ...
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bioRxiv - Neuroscience 2020Quote: (T-4)-[(1E,6E)-1,7-Bis[4-(dimethylamino)phenyl]-1,6-heptadiene-3’5-dionato-kO3’kO5] difluoroboron (CRANAD-2) [26] was purchased from Sigma-Aldrich AG ...
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bioRxiv - Immunology 2023Quote: ... #22363548) in a 6 well plate containing 1.35 mM 2’-deoxyguanosine (2-dG, Sigma-Aldrich) in complete DMEM-10 (DMEM ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the leaves from five-week-old Col-0 plants were sprayed 6 times over 48 h with methyl jasmonate (MeJA) (Sigma-Aldrich, Cat # 392707 ...
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bioRxiv - Cell Biology 2024Quote: ... islets isolated from chow-fed C57BL/6 WT mice were pre-incubated in 20 mM cholesterol loaded onto methyl-β-cyclodextrin (MβCD) (C4951, Sigma Aldrich) diluted in 1X Krebs-Ringer bicarbonate-Hepes (KRBH ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma Aldrich, St. Louis, MO, USA) diluted 1:3000 in PBS 1X ...
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bioRxiv - Cancer Biology 2022Quote: ... A21111) Slides were counterstained using 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL in PBS, Sigma-Aldrich, cat. D9542-5MG). Coverslips were mounted using mounting medium (Fluoromount-G™ Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 1x PBS and fixed on slide with methanol followed by DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich) to stain nuclear and kinetoplast DNA and analysed immediately ...