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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for 3 2 Methoxy phenoxymethyl piperidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Viability was evaluated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric assay (Sigma, #M5655).
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bioRxiv - Microbiology 2020Quote: Biofilms were set up as described previously except they were incubated at 37°C in either 0.03% CO2 or 5% CO2 for 48h in RPMI-1640 containing varying concentrations of the Fe2+ chelator 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p’-disulfonic acid monosodium salt hydrate (Ferrozine – Sigma-Aldrich, 160601) or the Fe3+ chelator Deferasirox (Cambridge Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading/transmigrating cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Biophysics 2022Quote: ... Both the CS and cantilevers were washed with acetone and functionalized using 2% (v/v) 3-aminopropyltriethoxysilane (Millipore Sigma) solution dissolved in acetone ...
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bioRxiv - Systems Biology 2022Quote: ... and then a certain amount of culture (2~3 mg dry weight) was filtrated through a biomass filter (Durapore 0.45 μm, diameter 25-mm, Sigma). The filter with the cell pellet was immediately transferred to an aliquot of 4 mL of precooled extraction solution (−20°C ...
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bioRxiv - Physiology 2022Quote: Cell viability was measured by the colorimetric assay showing reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) as previously described (Denizot and Lang ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich) as described [29].
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, San Louis, MI, USA) solution in PBS was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Biochemistry 2022Quote: ... Cholesteryl hemisuccinate (CHS, C6013) and 1-stearoyl-2- hydroxy-sn-glycero-3-phosphocholine (Lyso-PC) were purchased from Sigma. Brain phosphatidylinositol-4-phosphate (PI(4)P) ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of medium containing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (1 mg/mL) (Sigma-Aldrich) was added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... frozen tissue (from 2–3 mice or 1 rat) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... This assay relies on the reduction and conversion of yellow 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) reagent (#M2128, Sigma-Aldrich) into purple formazan salt ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 5 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma Aldrich) in PBS for 15 min at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... 2-3 eyes from different zebrafish were sonicated in RIPA lysis buffer containing protease inhibitor cocktail (Sigma, Cat# P2714). Protein samples were mixed with loading buffer and boiled for 5 minutes at 95-100°C ...
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bioRxiv - Physiology 2021Quote: ... 100 µl homogenate were added to 400 µl of ice-cold methanol/water 4:1 (v/v) / 0.2% formic acid and isotope-labeled internal standards (10 µl 2 µM 13C3,15N-3-hydroxykynurenine (Sigma-Aldrich) and 10 µl 2 µM D5-kynurenic acid (Eurisotop)) ...
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bioRxiv - Biochemistry 2021Quote: ... CGI-99(2-101)-containing peak fractions were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) to 24 mg ml−1 ...
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bioRxiv - Molecular Biology 2021Quote: ... gallinamide A-bound cruzain was concentrated to 10 mg/mL and the activation buffer was exchanged to 2 mM Bis-Tris pH 5.8 using a centrifugal filter with molecular weight cut-off of 3 kDa (Millipore). For cruzain-gallinamide A analog complexes ...
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bioRxiv - Microbiology 2020Quote: ... 2 mL of 0.3% agarose in DMEM supplemented with 2% FBS or 3 mL of 0.6 or 1.2 percent microcrystalline cellulose (MCC; Sigma 435244) in serum-free DMEM was added ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... washed in PBS and incubated in buffer A (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... After addition of 10μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) solution (5mg/ml, Sigma-Aldrich, USA) the cells were incubated in darkness for another 4 hrs ...
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bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
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bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was applied to the cells and incubated for an additional 4h ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL aliquots were taken from the main culture for chemical fixation with 3% paraformaldehyde (PFA, Sigma-Aldrich, #F8775) at 20 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
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bioRxiv - Microbiology 2023Quote: ... 30 μL of MTT [(3- [4,5-dimethyl-2-thiazolyl] -2,5-diphenyl-2H-tetrazolium bromide; 5 mg/mL; (Sigma-Aldrich)] was added in each well and the plate incubated for 3 hours at 25°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich (St Louis, MO). Phalloidin-iFluor 594 was purchased from Abcam ...
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bioRxiv - Microbiology 2024Quote: ... after which the remaining supernatant (2 x 45ml) was collected and concentrated over a 3 kDa Amicon filter (Millipore). The residue was suspended in a volume of 900 µl ...
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bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Molecular Biology 2023Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (MTT) cell growth kit (CT02, Millipore, MA, USA) was used for measuring cell viability ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Immunology 2023Quote: ... RIPA 400 mM NaCl, (3) Sarkosyl Buffer (2 mM EDTA, 50 mM Tris-HCl, 0.2% sarkosyl (Lauroylsarcosine sodium salt (Sigma)) ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in 80% ethanol for 2-3 minutes and differentiated in 0.1% lithium carbonate (Sigma #62470) for 45-60 seconds followed quickly by washes in distilled water to stop the differentiation ...
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bioRxiv - Microbiology 2023Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Microbiology 2023Quote: The effect of drug treatments on cell viability was assessed using the MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (the MTT powder was obtained from Sigma Aldrich). VTN or Huh-7 cells were seeded the day before treatment at a concentration of 7L×L103 cells per well in DMEM supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...
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bioRxiv - Cancer Biology 2022Quote: ... Five thousand cNF-derived primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated opaque 96-well plates in SCM without IBMX with Forskolin and maintained at 37ºC under a 10% CO2 atmosphere for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... One hundred thousand primary SCs from 3 independent patients (see Supplemental Table 2) were plated onto 0.1 mg/mL Poly-L-lysine (Sigma) and 4 μg/mL Laminin (Gibco)-coated 12-well plates in SCM without IBMX ...
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bioRxiv - Biophysics 2022Quote: ... Concentrated Cav1.2(ΔC)/Cavβ3/Cavα2δ-1 sample was immediately incubated with gabapentin (final concentration 2 mg ml-1, 11.7 mM) (Sigma-Aldrich) on ice for four hours prior to cryo-EM sample preparation ...
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bioRxiv - Developmental Biology 2023Quote: ... unlysed embryos were cultured during 2 to 3 hours in 40 µL micro drops of TCM199 (Sigma-Aldrich, M4530) + 10% foetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixed embryos were washed 3 times (5 minutes each) in wash buffer DPBS Tween containing 2% BSA (Sigma, A3311), and permeabilised at room temperature for 20 minutes in permeabilisation buffer 0.5% Triton-X in DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mg/ml of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich®, St Louis, MO, USA) was added and incubated at 37°C for 3h ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed at a 3:2 ratio with a crowding buffer [20% dextran (from Leuconostoc spp., Mr 450,000-650,000; Sigma 31392) and 1 mM DTT in 50 mM Tris-HCl pH 7.4] ...