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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... 4 μL 5 M Betaine (Sigma-Aldrich), 6.4 μL Water ...
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bioRxiv - Molecular Biology 2024Quote: ... native ChIP was performed using an increasing amount of H3K4me3 antibodies (for the Abcam 0, 2, 4, 6, 10μl, for the Diagenode 0, 4, 8, 12μl and for the Millipore 0, 2, 4, 8, 16μl). The number of gene-specific sequences associated with each quantity of antibody was quantified by real-time PCR targeting the gene encoding the 40S subunit (Forward primer ...
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bioRxiv - Bioengineering 2024Quote: ... we immersed the dried slides in an esterification mixture consisting of 200 mg 4-(dimethylamino)pyridine (cat. no. W284300, Sigma-Aldrich), 444 μL pentenoic acid (cat ...
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bioRxiv - Cell Biology 2023Quote: ... Bottom coverslips are activated with a 2% 3-Aminopropyltrimethoxysilane (3-APTMS) (Sigma Aldrich 13822-56-5) in 96% ethanol solution for 5 minutes and washed with 70% ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... gels were washed 3 times x 5 minutes with a 3% Bovine Serum Albumin (BSA, Sigma) blocking solution in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... or SC-Leu-Trp-His +5 mM 3-AT (3-Amino-1,2,4-triazole, Sigma, 18056-25G). Plates were incubated at 30°C and imaged daily for at least three days.
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bioRxiv - Immunology 2020Quote: ... 50 μl of antibody exposed to hemin or hemin in PBS only were mixed with 150 μl of reaction buffer (0.15 M citrate-phosphate buffer pH 5) containing 0.9 mM of 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma-Aldrich) and 6 mM H2O2 ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’) targeting exon 7 of ERBB2 (cloned by Twin Helix) and selected with puromycin (Sigma-Aldrich) for 4 days (1 μg/ml puromycin for 72 hours ...
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bioRxiv - Immunology 2022Quote: Murine lungs were perfused and fixed overnight at 4 °C in 4% paraformaldehyde (Merck Millipore) under agitation ...
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bioRxiv - Developmental Biology 2023Quote: ... gills were fixed overnight in 4% paraformaldehyde at 4°C (Sigma, St. Louis, MO, USA) in diethyl pyrocarbonate in PBS (PBS-DEPC) ...
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The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... washed in sterile 4°C dPBS (D8537, Sigma), pelleted again and dPBS was removed ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C in 10 kDa Amicon (Merck Millipore) filters ...
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bioRxiv - Neuroscience 2024Quote: ... Ara-C (4 mM; Sigma Aldrich, catalog# C6645), 10 ng/mL of BDNF ...
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bioRxiv - Molecular Biology 2023Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (MTT) cell growth kit (CT02, Millipore, MA, USA) was used for measuring cell viability ...
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bioRxiv - Neuroscience 2023Quote: ... The cell viability was measured by quantifying the activity of cellular dehydrogenases that reduce MTT (3-4,5-dimethyl thiazolyl-2,5-diphenyl-2H-tetrazolium bromide, Sigma Inc.) to a purple formazan salt [35] ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Biophysics 2021Quote: ... was performed using a pre-annealed DNA-RNA template (DNA45: 5’-TTCTTT ATCTCTTATTTCCTTCTATTTTCCACGCGCCTTCTATTT-3’; RNA13: 5’-[6FAM]-GGCGCGUGGAAAA-3’, Sigma Aldrich). The reaction buffer comprised 25 mM HEPES pH 7.2 ...
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bioRxiv - Cancer Biology 2022Quote: ... 53 from the RNAi Consortium (clones TRCN0000087290 and TRCN0000087292 with target sequence 5’-GCGGGAGTATAGTGAGTTTAA-3’ and 5’-CGGACAGTTTGGCACAATCAA-3’, respectively, distributed by Sigma-Aldrich, Merck ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were counterstained with 5 μM 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, Cat# D9542) in PBS ...
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bioRxiv - Immunology 2021Quote: ... in complete media containing 10 U/mL rhIL-2 with or without 10 or 20 uM 6-(4-Chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO) (Sigma-Aldrich); an equal volume of DMSO served as the negative control.
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 5 min with 10 μg/ml 4’,6-Diamidino-2-phenylindole (DAPI; Sigma-Aldrich) to stain cell nuclei ...
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Systemic administration of Ivabradine, an HCN channel inhibitor, blocks spontaneous absence seizuresbioRxiv - Neuroscience 2021Quote: ... and ELA (N-[4-[2-(3,4-Dihydro-6,7-dimethoxy-2(1H)-isoquinolinyl)ethyl]phenyl]-9,10-dihydro-5-methoxy-9-oxo-4-acridinecarboxamide) were purchased from Sigma-Aldrich. For systemic injections ...
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bioRxiv - Neuroscience 2023Quote: ... 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS, Sigma-Aldrich) substrate was added to each well ...
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bioRxiv - Genomics 2023Quote: ... with 3 μg of 6-OHDA in 0.01% ascorbate (Sigma–Aldrich) into the median forebrain bundle (MFB ...
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bioRxiv - Neuroscience 2024Quote: ... and the remaining pellet was washed once with 50 mM TBS, centrifuged (200,000x g, 22 min at 4 °C) and then solubilized in 6 M GuHCl (Sigma) with protease inhibitors ...
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bioRxiv - Genetics 2024Quote: ... Coverslips were washed twice for 30 min at 37°C with 2x SSC/10% formamide with 0.2 mg/ml DAPI (4’,6-Diamidin-2-phenylindol, Sigma) being added to the second wash ...
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bioRxiv - Biochemistry 2021Quote: ... pre-incubated for 3-7 h with rabbit anti-FLAG antibody (Sigma-Aldrich) at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... and 3-7 units/mL of pyruvate kinase and lactate dehydrogenase (Sigma P0294). An NADH standard curve was measured in order to convert absorbance values to the concentration of ATP hydrolyzed ...
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bioRxiv - Neuroscience 2020Quote: ... to 30 µL CSF (pooled from 1 – 3 animals) and placing in 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich). Serum samples (samples pooled correspondingly to the CSF samples ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 0.1% 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl)_(NBD-PE) in a 4:1 mixture of silicone oil (Sigma-Aldrich) and mineral oil (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... in pyridine (Sigma; 270970) was added and incubated in a thermomixer C (Eppendorf ...
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bioRxiv - Molecular Biology 2020Quote: - Pyridine (Cat# PX2012, Sigma)
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bioRxiv - Neuroscience 2021Quote: ... 1% pyridine (Sigma 270970), 1% butyric acid (Sigma W222119) ...
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bioRxiv - Plant Biology 2023Quote: ... in pyridine (Sigma-Aldrich). The resuspended sample was incubated at 37°C and shaken at 1800 rpm for 90 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... in pyridine (Sigma; 270970) was added and incubated in a thermomixer C (Eppendorf ...
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bioRxiv - Microbiology 2023Quote: ... in pyridine (Sigma, 270970) was added and incubated in a ThermoMixer (Eppendorf ...
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bioRxiv - Microbiology 2023Quote: ... in pyridine (Sigma; 270970) was added and incubated in a thermomixer C (Eppendorf ...
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bioRxiv - Developmental Biology 2022Quote: ... TS-45950):Pyridine (Sigma-Aldrich, 270970) followed by derivatization with 70μl of N-tert-Butyldimethylsilyl-N- methyl-trifluoroacetamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... in pyridine (Sigma Aldrich). Samples were briefly vortexed and centrifuged ...
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bioRxiv - Microbiology 2024Quote: ... in pyridine (Sigma; 270970) was added and incubated in a thermomixer C (Eppendorf ...
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bioRxiv - Plant Biology 2024Quote: ... and pyridine (Sigma-Aldrich) (1:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell lines were regularly passaged every 3-4 days by dissociation with Trypsin-EDTA 0.25% (Sigma Aldrich). The cell lines were regularly subjected to monthly screening for mycoplasma infection ...
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bioRxiv - Neuroscience 2020Quote: ... 6–8-week-old C57BL/6N mice were anesthetized by inhalation of isoflurane (3–4%) or intraperitoneal injection of 2% Avertin solution (2,2,2-tribromoethyl alcohol dissolved in Tert-amylalcohol (Sigma)) dissolved in saline ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissue sections were then treated with 3% H2O2 and nonspecific binding was blocked with 4% BSA (A7906; Sigma) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... polymyxin B sulfate and 2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) (Sigma-Aldrich Corp., St. Louis, MO) were dissolved in water ...