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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for 2 1 1 Diethoxy 2 methyl propyl 4' Nitrophenyl Carbonate d6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 2 and 3 emulsified 1:1 in Freund’s incomplete adjuvant (Sigma-Aldrich) on days 0 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal against alpha-tubulin (1:1000; b5-1-2, Sigma, T6074). Actin was stained using 100 nM of phalloidin-iFluor488 for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse tubulin (clone B-5-1-2, Sigma, T5168-.2ML; 1/5000), and p21 Waf1/Cip1 (clone 12D1 ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-Tubulin (1:3,000; Sigma clone B-5-1-2, T5168), and mouse anti-b-Actin (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... is added 1:1 together with 2 µg/mL doxycycline (Sigma-Aldrich) to induce TetO gene expression ...
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bioRxiv - Microbiology 2024Quote: ... + 2% HI FBS + 1% Anti-anti + 1% (w/v) carboxymethylcellulose (Sigma-Aldrich)) was added to the plate (180 µL/well) ...
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bioRxiv - Microbiology 2024Quote: ... + 2% HI FBS + 1% Anti-anti + 1% (w/v) carboxymethylcellulose (Sigma-Aldrich)) was added to cells ...
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bioRxiv - Plant Biology 2024Quote: ... anti-microtubule antibody (1:2,000; clone B-5-1-2; Sigma- Aldrich), and ECL anti-mouse IgG horseradish peroxidase-linked whole antibody (1:1,000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... 2 DIV differentiated cells were treated with 1 µM CDK1/2 inhibitor III (Millipore, 217714) in control medium supplemented with 2% FBS for 24 h ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in pre-chilled 1mL of 2:2:1 acetonitrile (34851, Millipore Sigma) + methanol (439193 ...
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bioRxiv - Biophysics 2020Quote: ... (D9891), 2-aminoethoxydiphenyl borate (2-APB) (D9754) and poly(methyl methacrylate) (PMMA, Mw~15,000) (200336) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Thapsigargin (586005 ...
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bioRxiv - Biochemistry 2022Quote: ... they were cultivated 72 hours in the presence of 2 μM DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) (Sigma-Aldrich) to inhibit the synthesis of glucosylceramide-based GSLs 75 and incubated with fluorescent SaroL-1 as previously described ...
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bioRxiv - Neuroscience 2022Quote: ... For the blockade of cholinergic receptors in CA1 200nl of atropine (2 mM) and mecamylamine (2 mM) (1:1) (Sigma Aldrich) were microinjected at 1.2mm DV (IM-9B microinjector ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with anti-Sulf-1/2 primary antibody (anti-Sulf-1: rabbit polyclonal, Sigma, SAB1410410, anti-Sulf-2: rabbit monoclonal clone 2B4, Sigma, MABC584) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of 2 mg/mL IG[pS]TENLK was mixed 1:1 with 250 mM Ba(OH)2 (Sigma-Aldrich) in a total volume of 200 μL and incubated at 37°C for 90 min ...
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bioRxiv - Bioengineering 2024Quote: The alkaline comet assay was performed by mixing 1.5 × 105 cells 1:1 with 2 % low melting point agarose (2-Hydroxyethylagarose, Type VII, Sigma-Aldrich, A4018) at 37°C ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... The plates were washed using ELISA washing solution followed by addition of substrate (4-Nitrophenyl phosphate disodium salt hexahydrate, Sigma-Aldrich, 1 mg/ml), 150 µl/well ...
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bioRxiv - Cell Biology 2023Quote: N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM, Sigma) was dissolved in DMSO at 4 mg/ml as stock solution and diluted 20 times using a buffer containing 25 mM HEPES ...
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Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... of liver homogenate (0.08 M NaPO4) was mixed with 30 μL of p-nitrophenyl-2-acetamide-β-D-glucopyranoside (Sigma-Aldrich) and diluted in 50 μL of 50 mM citrate buffer ...
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bioRxiv - Microbiology 2020Quote: ... and inositol phosphates were eluted by adding 1 ml of 100 mM ammonium carbonate (Sigma) and equilibrated with 0.5 mL of 5% acetic acid ...
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bioRxiv - Cell Biology 2022Quote: ... 3-(Trimethoxysilyl)propyl methacrylate (Sigma), and 96% ethanol (1:1:14 ...
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bioRxiv - Developmental Biology 2024Quote: ... 3-(Trimethoxysilyl) propyl methacrylate (Sigma), and 96% ethanol (1:1:14 in volume ratio ...
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bioRxiv - Cell Biology 2024Quote: ... N-propyl gallate (Sigma, 02370) was added to the agarose pads at 0.1mM final concentration to prevent photobleaching75.
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bioRxiv - Physiology 2023Quote: ... and 10 μM deoxycholic acid-d6 (Sigma) in LC−MS-grade methanol was prepared as internal standard spiking solution (ISSS) ...
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bioRxiv - Neuroscience 2024Quote: ... N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP-4) (C8417, Sigma-Aldrich, USA) is a selective neurotoxin targeting the noradrenergic system in the locus coeruleus of the brain ...
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bioRxiv - Cancer Biology 2022Quote: ... Dactolisib was freshly solved in N-Methyl-2-Pyrrolidone (NMP; Sigma-Aldrich, Darmstadt, Germany, Cat# 328634) and Polyethylene glycol 300 (PEG300 ...
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bioRxiv - Cell Biology 2024Quote: The grids were subsequently rinsed and embedded in a solution of 2% methyl cellulose (Sigma-Aldrich) and 0.4% uranyl acetate (EMS) ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 4 min with 5% w/v sodium carbonate (S7795, Sigma-Aldrich) in 3.7% neutral buffered formaldehyde ...
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bioRxiv - Neuroscience 2023Quote: ... slides were rinsed thrice with 1 x PBS and then stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10000, Sigma-Aldrich, D9542-10MG) in 1 x PBS solution ...
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bioRxiv - Molecular Biology 2024Quote: ... native ChIP was performed using an increasing amount of H3K4me3 antibodies (for the Abcam 0, 2, 4, 6, 10μl, for the Diagenode 0, 4, 8, 12μl and for the Millipore 0, 2, 4, 8, 16μl). The number of gene-specific sequences associated with each quantity of antibody was quantified by real-time PCR targeting the gene encoding the 40S subunit (Forward primer ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa strains were routinely refreshed from frozen stocks at -80°C and maintained on YPD (1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, 2% Bacto agar) plates or Pseudomonas isolation agar (Sigma-Aldrich) for in vitro experiments and LB agar (10 g/liter Bacto tryptone ...
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bioRxiv - Developmental Biology 2020Quote: ... the nuclei were labeled with a 1:10,000 dilution of DAPI (4’,6-Diamidino-2-phenylindole; Sigma, D9542) in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich catalogue no D9542, 1:600 dilution). Figure 4 shows images of seedlings from 1 of 6 experiments ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were stained by addition of 1 µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) containing FluoromountG (Southern Biotech) ...
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bioRxiv - Cancer Biology 2022Quote: ... the cover slides were treated with 1 mg/ml N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate (Sigma) and exposed to ultra-violet (UV ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:10,000 dilution with milliQ water, Sigma-Aldrich) for 10 minutes ...
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bioRxiv - Physiology 2020Quote: ... Nuclei were counter-stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 1/5000) (Sigma-Aldrich, Dorset, UK). Imaging was performed on a Leica TCS SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Immunology 2020Quote: Cell suspensions were stained for viability with either 1:3000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) or 1:1000 Zombie Aqua (Biolegend ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a titration of trimethoprim (0, 1, 2, 4, 8, 16, 24, and 32 µg/mL) (Sigma Aldrich) with 0.4 % dimethylsulfoxide ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) 1:1,000 (Sigma-Aldrich Sweden AB, Stockholm, Sweden). Invasion of the cells was measured by counting the nuclei of the cells that migrated through the filter pores towards the chemoattractant (6% FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... with 1:30,000 of the fluorescent marker of nucleic acids 4’,6-diamino-2-fenilindol (DAPI; Sigma-Aldrich). We took photographs of the cells using an epifluorescence microscope (DMIL LED ...
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bioRxiv - Biochemistry 2022Quote: ... β-mercaptoethanol and HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid] were procured from Sigma (St. Louis, MO, USA). Urea and proteinase-K were bought from Amresco ...
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bioRxiv - Physiology 2022Quote: ... a 4 uL suspension of 2 μm red-fluorescent polystyrene microspheres (Sigma-Aldrich; 1:1000 dilution in PBS) was apically applied to native mucus ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated with DAPI (4’,6-Diamidino-2-phenylindole dihydrochlorid) solution(D9542, Sigma-Aldrich, diluted 1:10000) for 7 minutes at RT and subsequently mounted in ProLong Gold Antifade mounting medium (cat ...
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bioRxiv - Pathology 2024Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, 1:10,000; Sigma-Aldrich; Saint Louis, MO, USA) and then ...
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bioRxiv - Bioengineering 2024Quote: ... was dispersed in 2 mL 4:1 (volume ratio) mixture solvent of cyclohexanone (Sigma-Aldrich, Product Number: 398241) and terpineol (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Chemical compounds were first identified using the NIST library and later confirmed with co-elution of synthetic 4-methyl-3-heptanone (Pfaltz and Bauer M19160) and 4-methyl-3-heptanol (Sigma Aldrich M48309). Compounds eluting after 30 minutes were excluded from the analysis due to lack of volatility.