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Citations for Millipore Sigma :
6901 - 6950 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a PVDF membrane (Millipore Immobilion-P, IPVH00010) using a wet transfer with freshly made transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... For labeling an Atto 488 protein labeling kit (Sigma-Aldrich, 38371) and an Atto 647N protein labeling kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated on Amicon Ultracel 10K centrifugal filters (Millipore) to 23 mg/ml concentration.
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bioRxiv - Neuroscience 2024Quote: ... Protein fragments were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen) cells ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were denatured with 5 % v/v 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in gradient Bolt Bis-Tris gels (Thermo) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced with isopropyl-b-D-thiogalactopyranoside (IPTG, Millipore) at 100 uM and cells were grown overnight at 16°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were harvested with RIPA lysis buffer (Sigma Aldrich; R0278) containing protease inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: Proteins were isolated from the liver using CelLyticTM MT (Sigma, C3228) in the presence of a protease inhibitor/phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to Immobilon-P PVSF (0.45µm, Sigma-Millipore) in Tris-Glycine buffer supplemented with 20% methanol for 1 h at 75V ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to Immobilon-P PVSF (0.45µm, Sigma-Millipore) in Tris-Glycine buffer supplemented with 20% methanol for 1 h at 75V ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extract was incubated with anti-FLAG Affinity gel (Sigma-Aldrich)/ Myc-Trap Agarose (ChromoTek ...
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bioRxiv - Plant Biology 2024Quote: ... and proteins were detected with Western HRP substrate (Immobilon Millipore, USA) and visualized with a CHEMI premium imager (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... chicken IgY anti-glial fibrillary acidic protein (GFAP) (EMD Millipore, AB5541), rabbit IgG anti-Iba1 (Wako Chemicals ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were then transferred to YM-10 Microcon filtration devices (Millipore) and centrifuged at 14,000 g for 15 min ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration of proteins was measured with Bradford reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... rabbit anti-Enterobacterio Phage MS2 Coat Protein (Millipore-Sigma, ABE76-I), rabbit anti-CRBN (sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: Protein samples were reduced in 10 mM dithiothreitol (DTT, Sigma-Aldrich) at 20 °C for 60 min ...
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bioRxiv - Microbiology 2024Quote: ... lysates were incubated with PureProteome protein A/G magnetic beads (Sigma) for 1-2 h ...
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bioRxiv - Microbiology 2024Quote: ... Separated proteins were transferred onto polyvinylidene difluoride membranes (Immobilon P; Millipore). The membranes were treated with anti-6×His antibody diluted 1:5,000 in 1× Tris-buffered saline containing 0.05% Tween 20 (TBST) ...
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bioRxiv - Neuroscience 2024Quote: ... Separated proteins were transferred to Immobilon-PSQ PVDF (Millipore, Billerica, MA) membranes overnight at 9 V at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Proteins were visualized on membranes using Ponceau S solution (Sigma-Aldrich) prior to 30 min blocking with (PBS ...
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bioRxiv - Immunology 2024Quote: ... immunoprecipitation was performed using Protein G Sepharose Fast Flow beads (Sigma). After 3 washes in lyses buffer ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a PVDF membrane (Sigma Aldrich, Germany). The membranes were blocked for 1 h in Tris-buffered saline containing 0.05% Tween 20 (TBS ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA) and then blocked with 5% nonfat dried milk for 1 h at room temperature (RT) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to Immobilon-FL PVDF membrane (Merck Millipore, IPFL00010) at 100 volts using 1x Towbin transfer buffer with 20% methanol ...
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bioRxiv - Molecular Biology 2024Quote: ... and Etanercept chimeric proteins (Millipore Sigma Cat No. 185243-69-0), representing the extracellular domain of the target receptor fused to the Fc region of immunoglobulin G ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were transferred to a membrane (Immobilon-FL PVDF, IPFL00010, Millipore), stained with Revert 700 Total Protein Stain (926-11011 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Merk-Millipore, IPFL00010), blocked with SuperBlock Blocking Buffer in PBS (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the FLAG interacting protein mix was eluted by 3xFLAG peptides (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to polyvinylidene fluoride membrane (Millipore, Cat# ISEQ00010) following by incubation with blocking buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Equal protein loading was confirmed using Ponceau S Staining (P7170, Sigma). Blots were stained using indicated antibodies (Supplementary Table 2) ...
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bioRxiv - Pathology 2024Quote: ... Transfer the proteins onto a PVDF membrane (ISEQ00010, Merck Millipore, Germany). Block the membrane with 5% skim milk for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... we diluted fluorescently tagged BSA protein (Sigma-Aldrich, St. Louis, MO) to a concentration of 0.1 mg/mL and encapsulated the solution in 45-μm droplets using the single-emulsion droplet-generation chip ...
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bioRxiv - Biochemistry 2024Quote: ... A dilution series of the protein synthesis inhibitor cycloheximide (Sigma 01810) was created in water ...
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bioRxiv - Biochemistry 2024Quote: Fusion proteins were expressed in BL21(DE3)pLysS E.coli (EMD Millipore). The proteins were tagged at the N-terminus with his6-HT7 (pET30-HT7 vector) ...
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bioRxiv - Bioengineering 2024Quote: Proteins were extracted from fibroblast cells using RIPA Buffer (Sigma #R0278) with protease inhibitors (Roche #11836153001) ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentrated protein underwent further processing with centrifugal filter units (Millipore). The purified SsCBE2-C2 protein was then stored at -80°C.
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bioRxiv - Bioengineering 2024Quote: ... Protein concentration was calculated following Bradford assay (Bradford reagent, Sigma-Aldrich). A 1:50 mixture of β-mercaptoethanol (Acros Organics ...
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bioRxiv - Neuroscience 2024Quote: ... protein lysates were treated with 5 mM dithiothreitol (DTT; Sigma-Aldrich) and heated to 90°C for 10 minutes for reduction ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then incubated with LysC and trypsin (Sigma cat. # T6567) at 37°C for 18 hours to promote digestion ...
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bioRxiv - Biophysics 2024Quote: ... protein expression was induced with 100 ng/mL doxycycline (Sigma-Aldrich). Cells were incubated for a further day before performing live-cell microscopy experiments.
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bioRxiv - Plant Biology 2024Quote: ... Stroma proteins were concentrated with Amicon Ultra Centrifugal Filters (Merck Millipore). The protein content was estimated using Bradford Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... proteins were electroblotted onto Polyvinylidene Difluoride (PVDF) membranes (Sigma, 05317-10EA) at a constant 60 volts for two hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µL of protein extract dilutions and 25µL of Nitrocefin (Sigma) 50 % (0.25 mg/mL ...
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bioRxiv - Biophysics 2024Quote: ... the cDNA of the fusion protein was cloned into pET21a (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Protein loading was verified by staining with Ponceau S solution (Sigma).
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bioRxiv - Pathology 2024Quote: ... had porcine pancreatic elastase (Sigma, Type I, ≥4.0 units/mg protein) applied topically to their infrarenal aorta and injected 3 times/week I.P ...
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bioRxiv - Synthetic Biology 2024Quote: ... Pellets were resuspended in 300 µL BugBuster Protein Extraction Reagent (Novagen), rocked at room temperature for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions were concentrated using a 30 K MWCO protein concentrator (Millipore) and applied to size exclusion chromatography (Superdex™200 Increase 10/300 ...
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bioRxiv - Biophysics 2024Quote: ... The cell was first filled with Protein A (Sigma P6031-1MG) solution (20 μl ...