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Citations for Millipore Sigma :
6851 - 6900 of 7003 citations for CYBB Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The in situ immunolocalization used these antibodies: anti-PIN1 (1:1000) and secondary goat anti-rabbit antibody coupled Cy3 (Sigma-Aldrich, 1:600). Confocal imaging was carried out on a Zeiss LSM 800 microscope (Cy3 ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed again as above and 100 μl of a goat anti-rabbit IgG (H + L) alkaline phosphatase conjugate (Sigma, St. Louis, MO) diluted 1:2,000 in PBS/1% NFM were added to each well and incubated for 1.5h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The membranes were washed with 1×TBST for three times followed by incubation with a streptavidin-horseradish peroxidase (1:2000, streptavidin-HRP) or an anti-rabbit antibody conjugated with HRP (1:2000, Sigma, Cat#12-348) at room temperature for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 72-hours in the same blocking solution with rabbit anti-CRF (Peninsula,T-4037, 1:1K) and mouse anti-TH (Millipore, MAB318, 1:5K) at 4°C on a shaker followed by 24-hours at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Biophysics 2023Quote: ... Sections were cut (5 µm) and immunolabeled as described above using a rabbit polyclonal anti-Cx43 antibody (Sigma-Aldrich C6219, St. Louis, MO) followed by goat anti-rabbit Alexa 568 (1:4000 ...
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bioRxiv - Neuroscience 2023Quote: ... nonspecific binding sites were blocked and slices were incubated overnight with a rabbit anti-tyrosine hydroxylase (TH) (1:1000, Millipore Cat# AB152, RRID:AB_390204), a rat anti-DAT (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with 0.05% Tween-20 in phosphate buffered saline (PBST) for 3 × 5 min with agitation and incubated with anti-β actin (1:1000 rabbit polyclonal (Sigma Aldrich; A2066) or 1:5000 mouse monoclonal (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... slow developmental (S46, 1:50; Developmental Studies Hybridoma Bank; Iowa City, IA) and rabbit anti-NF-H (1:500; EMD Millipore; Burlington, MA) for 24 hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then washed in PBS and placed in a humid chamber to be stained with the primary antibody anti-HA tag at a concentration of 8 μg/mL (anti-rabbit, #H6908, Sigma-Aldrich, Deisenhofen, Germany) in PBS ...
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bioRxiv - Microbiology 2023Quote: ... and probed with the appropriate primary antibody diluted in TBS-T with 3% BSA: rabbit anti-HA (Sigma Aldrich #H6908; 1:3,000 dilution), mouse anti-FLAG (Sigma Aldrich #F1804 ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated overnight with primary antibody (rabbit anti-REST1, Millipore CS200555, 1:1000, or mouse anti-GAPDH, Millipore MAB374, 1:500) in TBST with 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... the blot was probed for HA-epitope (rabbit anti-HA, Cell Signaling #3724, 1:1000; or rat anti-HA, Sigma #11867423001, 1:1000), or PSD95 (mouse anti-PSD95 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Immunology 2023Quote: ... diluted in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich in PBS) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and immediately incubated at 4°C in the primary antibody against the N-terminus of human Fos (overnight, 1:2000; Rabbit polyclonal, ABE457, Millipore; RRID: AB_2631318 (56) (Exp ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416, RRID:AB_258167; anti-rabbit, Sigma-Aldrich Cat# A6154, RRID:AB_258284) (1/2000) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 1h in a blocking solution of 0.01 M PBS with 0.25% Triton-X and 3% normal goat serum (NGS) and then incubated overnight at 4 °C with rabbit anti-NeuN antibody (1:2000, MAB5504, Millipore, Temecula, CA, USA) in 0.01 M PBS with 0.25% Triton-X and 3% NGS ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with a biotinylated secondary antibody (anti-mouse: horse, BA-2000, Vector Laboratories, Burlingame, USA; anti-rabbit: goat, B8895, Sigma-Aldrich, USA), diluted 1:1000 ...
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bioRxiv - Physiology 2024Quote: ... and the membrane was incubated for 2h at RT with rabbit anti-GAPDH antibody (Sigma Aldrich, G9545, diluted 1:2000 in TBS-T) at RT ...
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bioRxiv - Immunology 2023Quote: ... After incubating with secondary antibodies Alexa Fluor 488-conjugated goat anti-mouse IgG (1:3000 dilution) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:3000 dilution) together with DAPI (Sigma-Aldrich, Cat# D9542) at the final concentration of 1 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Neuroscience 2023Quote: ... blocked with 5% FBS and stained overnight at 4°C with primary antibodies for immunocytochemical analysis (rabbit anti-OCT4, 1:100, Proteintech, Cat. no. 11263-1-AP / mouse anti-TRA-1-81, 1:500, Millipore, Cat. no. MAB4381). Samples were visualized after staining with Alexa Fluor 488-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti-TH antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 44-48 hr ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were probed with either rabbit anti-RACK1A (1:500) (Chang et al., 2005) or mouse anti-α-Tubulin (1:3000) (Sigma-Aldrich T6074) antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times in PBS for 15 min and incubated in blocking solution containing rabbit anti DsRed polyclonal antibody (1:1000; EMD Millipore, Burlington, MA) with gentle agitation at 4°C for 18-22 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used: rabbit anti-Myosin7a (Proteus 25-6790; 1:1000) mouse anti-pan-Maguk (IgG1) (Millipore MABN7; 1:500); mouse anti-Ribeyeb (IgG2a ...
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bioRxiv - Developmental Biology 2024Quote: ... the cultures were washed 3 times in PBS for 5 minutes and incubated with Duolink Plus and Minus PLA probes (diluted 1:5) followed by Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, cat. no. DUO92102), according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was washed with TBST buffer and the membrane was set for 2 hours at room temperature with the secondary goat anti-rabbit IgG HRP antibodies (Sigma Aldrich; 1:10000) diluted in a TBST buffer with 3% PVP ...
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245, 1:1000; Rhodamine Phalloidin, Fisher Scientific 50-646-256, 1:200; DAPI, Sigma Aldrich 10236276001 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation in either sheep anti-mouse or donkey anti-rabbit IgG-conjugated with horseradish peroxidase (HRP) (Millipore Sigma, GENA931, GENA934, 1:4,000) in TBST at room temperature for two hours ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate [EMD Millipore AP160P], Rabbit IgG-HRP linked [EMD Millipore 12-348]) for 1 hr at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... The blot was cut in half below the 53K molecular weight marker and the top half probed with rabbit anti-tubulin (Sigma, Lot#50K4813, 1:20,000) and goat anti-rabbit IgG (HRP ...
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bioRxiv - Neuroscience 2019Quote: ... One mid rostro-caudal IC section from each animal was immunolabelled for GABA (rabbit anti-GABA antibody 1:1000, Sigma-Aldrich Cat# A2052, RRID:AB_477652), and the neuronal marker NeuN (mouse anti-NeuN antibody 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed in TBST 2 × 15 min and incubated for 1 hour in room temperature with HRP-conjugated anti-rabbit antibody (Sigma-Aldrich, NA9043, 1:2,500) diluted in TBST with 5 % non-fat dry milk ...
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bioRxiv - Cancer Biology 2020Quote: ... This was followed by incubation with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgGs or goat anti-mouse IgGs (both Sigma-Aldrich, USA; 1:10000). Protein bands were visualized using SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Cells were incubated at RT for 3 hrs in 300 μl secondary antibody solution containing anti-rabbit TRITC (Sigma-Aldrich Cat# T6778, RRID: AB_261740) at a concentration of 1:75 in 1× TBS ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated for 24 hours at RT on a rocking platform with ATTO647N-conjugated anti-rabbit antibodies (Sigma-Aldrich, catalog no. 40839) diluted to 1:250 in the antibody dilution buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Ribosomal protein L3 was detected using anti-L3 primary antibodies (a gift from Fujio Kawamura; 1:20,000 dilution) combined with goat anti-rabbit IgG-HRP secondary antibodies (Sigma-Aldrich, A0545; 1:10,000 dilution). ECL detection was performed using WesternBright™ Quantum (K-12042-D10 ...
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bioRxiv - Plant Biology 2021Quote: ... diluted anti-YFP antibody or other specific antibodies followed a 1:10,000 (v/v) diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or a goat anti-mouse antibody (Sigma-Aldrich, St. Louis, MO, USA). The detection signal was developed using the ECL substrate kit as instructed (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 3% (v/v) normal horse serum in TBS) for 1 hour at room temperature followed by incubation in rabbit anti-TH (Millipore Cat# AB152, RRID: AB_390204) (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal antibody directed against Iba-1 (FUJIFILM Wako, dilution 1:500) and mouse monoclonal antibody directed against GAD67 (Millipore Sigma, dilution 1:500). For the secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: Labeling of septins on Western blots (WB) and/or on immunocytochemistry coverslip (ICC) was achieved with the following antibodies: rabbit polyclonals against human Sept2 (Sigma-Aldrich, Cat#HPA018481, WB), against human Sept9 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were subsequently incubated with peroxidase-conjugated secondary antibodies (anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P; anti-rabbit IgG, HRP-linked, EMD Millipore, 12-348) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the bound (B) and unbound (UB)/flow through were analyzed by Western blotting using anti-GST rabbit polyclonal antibody (Sigma #G7781, 1:5000 dilution).
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bioRxiv - Cell Biology 2022Quote: ... CRISPR/Cas9-edited lines were subjected to staining with the Duolink™ In Situ Detection Reagents Orange (DUO92007) and the combination of α-mouse and α-rabbit probes (DUO92002 & DUO92004) from Sigma-Aldrich, as per manufacturers’ recommendations ...
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bioRxiv - Pathology 2022Quote: ... The sections were blocked using 1% BSA for 1 hr and incubated with rabbit anti-hamster ACE2 antibody (Product no. HPA000288, Millipore Sigma, Burlington, MA, USA) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... samples were further incubated overnight with secondary antibodies (rabbit or mouse) that were bound to PLA plus or minus complementary probes (Millipore-Sigma, supplementary table S7). The PLA probes were ligated when the proteins were in proximity due to their interaction giving a fluorescent signal as we previously reported (24) ...
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bioRxiv - Neuroscience 2021Quote: ... washed twice in PBS and incubated overnight at 4°C in primary anti-KCC2 raised in rabbit (1:1000, Millipore/Upstate, Cat. #07–432), anti-CGRP raised in mouse (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...