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Citations for Millipore Sigma :
6651 - 6700 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or 12% SDS– PAGE gels before transfer to Immobilin-P polyvinyl difluoride membrane (Millipore). Membranes were blocked in 5% non-fat milk in TBS-T and analyzed using standard immunoblotting protocols.
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bioRxiv - Cell Biology 2020Quote: ... lipids mixture dissolved in chloroform / methanol solution was gently applied to indium-tin-oxide coated glass slide (Sigma Aldrich Cat. No. 636908) as multiple layers ...
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with crystal violet (1% w/v in 20% methanol, Sigma, cat# C0775) for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... for wild type or mutant BAG3-GFP and fixed in ice-cold methanol (67-65-1, Sigma-Aldrich, Saint Louis, MI, USA) for 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and either placed in 19x volume of tissue weight of DPBS with 1x phosphatase inhibitor / protease inhibitor solution for protein quantification and immunoblot analysis or combined with 19x volume of tissue weight of ice-cold 60% methanol (LCMS grade, EMD Millipore Corporation) containing 0.01% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... 17-% methanol and was adjusted to pH 4.5 by phosphoric acid (all reagents were purchased from Sigma Aldrich, St. Louis, MO, USA), in line with 73 ...
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bioRxiv - Microbiology 2022Quote: ... 1 or 2 mL culture (corresponding to 10 mg natC-cell dry weight) was quenched in the 4-fold volume of quenching solution (60% methanol (Sigma-Aldrich GmbH), 125 mmol L-1 TRIS-HCl (Carl Roth GmbH) ...
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bioRxiv - Bioengineering 2019Quote: ... PET slips were washed with methanol and then ethanol three times and thoroughly dried before being treated with 1%glutaraldehyde (Sigma-Aldrich G7776) in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2019Quote: ... 24 and 48 hours and then fixed with 4°C methanol for 3 minutes and stained with safranin (Sigma-Aldrich, Gilligham, UK) for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Extraction of metabolites from tissue samples was conducted with a mixture of 80% v/v methanol (CHROMASOLV®; Sigma-Aldrich, Gillingham, UK), and 20% v/v water (CHROMASOLV® ...
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bioRxiv - Biochemistry 2021Quote: ... Non-invading cells were removed using a damp cotton swab while the invading cells were fixed in methanol for 10 mins and stained with 0.5% crystal violet (Sigma, St. Louis, MO) stain ...
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bioRxiv - Developmental Biology 2021Quote: The proteins in the sample were precipitated using methanol/chloroform and were resuspended in 6 M Guanidine HCl (Sigma cat# G3272-1KG), buffered using 50 mM EPPS (Alfa Aesar cat# A13714) ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized in ice-cold methanol by dipping the coverslip in it 20 times followed by blocking in 3% BSA (Sigma-Aldrich; A9647) in PBS for 15 min (Figures 2 A-H and S1) ...
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bioRxiv - Genomics 2022Quote: ... the entire area of the glass slide was covered in a methacarn solution (60% absolute methanol, 30% chloroform stabilized with ethanol and 10% glacial acetic acid (all from Sigma-Aldrich, USA)) for 10min at RT in a closed space to avoid evaporation ...
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bioRxiv - Neuroscience 2022Quote: ... Culture plates were placed on a bed of crushed dry ice and 1mL of ice cold 50% methanol (HPLC-grade, Sigma #A456-4) was added to quench cellular metabolic activity followed by a 10 minute incubation at −80°C to ensure cell lysis ...
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bioRxiv - Molecular Biology 2023Quote: Total fatty acids were extracted from the liver using a 1:2 (v/v) mixture of chloroform/methanol in the presence of 10 nmol of tridecanoic acid (Sigma-Aldrich #T0502) and 10 nmol of phosphatidylcholine (PC ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting lysate was mixed with water with a final concentration of 50% methanol and filtered through a 3,000Da MW/CO exclusion column (Amicon Ultra, Millipore, Cat. No. UFC500396) by centrifugation at 13,000 x g and -2 °C for 30-60 minutes to remove contaminating high molecular weight species.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Leuven, Belgium), distilled water (Ethiopian Pharmaceutical Manufacturing Factory, Ethiopia), absolute methanol (Indenta chemicals, India) and glacial acetic acid (Sigma-Aldrich laborchemikalien, Germany), indomethacin (Cadila pharmaceuticals Ethiopia) ...
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bioRxiv - Immunology 2023Quote: ... Ear skin was dehydrated and rehydrated in graded methanol and then incubated in 0.1% toluidine blue dye (Sigma Aldrich, St. Louis, MO). The dye was extracted with a buffer consisting of 95% methanol ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for ms-gamma-tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig-CENP-C 1:250 (MBL ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours post-knockdown and induction coverslips were fixed in -20oC methanol for 3 minutes and stained for ms-gamma tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig CENP-C 1:25 (MBL ...
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bioRxiv - Genomics 2023Quote: ... Cells were resuspended in 1X PBS and cross-linked for exactly 10 min using 37% (w/v) formaldehyde containing 10-15% (v/v) methanol (Sigma, Cat # 252549) at a final concentration of 2% (v/v) ...
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bioRxiv - Cancer Biology 2023Quote: ... Pre-cooled extraction buffer consisting of methanol: acetonitrile: H2O (40:40:20, V/V) with 0.5% formic acid (Sigma-Aldrich, # F0507-100ML) was added to the resulting powder (40 μL of solvent per mg of tumors) ...
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bioRxiv - Systems Biology 2024Quote: ... transferred onto nitrocellulose membrane (Amersham #10600041) in transfer buffer (8 mM Tris, 30 mM Glycine, 20 % Methanol) and stained with Ponceau S (Sigma-Aldrich, #P7170). Membranes were blocked in TBS-T + 5% BSA for 1 hr at RT and incubated overnight at 4°C in primary antibodies prepared in TBS-T + 5% BSA ...
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bioRxiv - Immunology 2024Quote: Four whole bodies of adult female flies were homogenised in 160 µl of 80% methanol (Wako, 132-06471) containing 10 µM internal standards (methionine sulfone (Sigma, M0876-1G) and 2-morpholinoethanesulfonic acid (Dojindo ...
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bioRxiv - Immunology 2024Quote: ... The extract was reconstituted in a 500-μl solution of methanol/water (1:1) and filtered through a 3-kDa molecular filter (Amicon® Ultra Centrifugal Filter, 3 kDa MWCO, Millipore Sigma) at 4°C for 90 minutes to remove proteins ...
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bioRxiv - Biochemistry 2021Quote: ... transferrin from human serum, (Tf), cytosine (Cyt), trifluoroacetic acid (TFA, ≥99%), and formic acid (FA, analytical grade >98%) were purchased from Sigma Aldrich (St. Louis, Missouri, United States). Methanol (MeOH) ...
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bioRxiv - Neuroscience 2023Quote: ... animals received the first of 6 injections of either vehicle (propylene glycol) or progesterone (>99% progesterone, purified from rats, Sigma-Aldrich Co. St. Louis, MO, USA) at a dose of 4 mg/kg (Shear et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Nifedipine (100 mM; DMSO) and atropine (100 mM; 100% ethanol) were purchased from Sigma-Aldrich and dissolved as described ...
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bioRxiv - Physiology 2022Quote: ... Nifedipine (100 mM; DMSO) and atropine (100 mM; 100% ethanol) were purchased from Sigma-Aldrich and dissolved as described ...
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bioRxiv - Plant Biology 2020Quote: ... water was removed and replaced by 100 µl of the assay solution: 17 µg/mL Luminal (Sigma-A8511, USA), 10 µg/ml Horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... Plates were sealed by piping a border of vaseline and 5% (v/v) mineral oil (Sigma, #M841-100 ml) and covered with a 22×22 Fisherbrand Cover Glass (# 12-541-B) ...
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bioRxiv - Molecular Biology 2021Quote: ... NMD inhibition by cycloheximide was performed by treating the cells for 1 hr with cycloheximide (Sigma, 100 μg/mL).
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bioRxiv - Developmental Biology 2021Quote: Pregnant CD-1 females were injected intraperitoneally at E10.5 with 100 μl of 16 mg/ml busulfan (Sigma-Aldrich) dissolved in 50% DMSO or an equivalent volume of 50% DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... in combination with 100 μg/ml DNase I in Pipes based buffer containing 1 mM Pipes (P1851, Sigma Aldrich), 25 mM L-Cystein HCL ...
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bioRxiv - Molecular Biology 2021Quote: ... NMD inhibition by cycloheximide was performed by treating the cells for 1 hr with cycloheximide (Sigma, 100 μg/mL). For ASO treatment by free-uptake ...
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bioRxiv - Neuroscience 2021Quote: ... in 16-well stripes (500,000 cells/well) and re-plated on glass coverslips coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... in 16-well stripes (500,000 cells/well) and re-plated in plastic dish (Costar) coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was washed in a pre-hybridization solution (6 x SSC, 1 x Denhardt’s, 100 μg/ml denatured Herring Sperm DNA (Sigma) and 0.5 % SDS ...
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bioRxiv - Biophysics 2019Quote: ... mixed with 100 mL sterile filtered buffered phosphate (0.17 M KH2PO4 (Sigma Aldrich, cat# P0662, CAS: 7778-77-0), 0.72 M K2HPO4 (Sigma Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... A 2 mL volume of warm virus medium (1X MEM with 100U/100 μg/mL penicillin/streptomycin, 0.3% bovine serum albumin [Sigma]) was added to each well ...
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The spheroplasts were then washed 3 times in SPM buffer permeabilized by incubation in 1 mL of 0.1% triton X-100 (Sigma) in PBS for 2 minutes on ice and washed twice in PBS ...
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bioRxiv - Microbiology 2019Quote: ... Chlorophyll a (Chl a) was determined in triplicate by filtering 100 mL of seawater onto 0.45 μm pore-size HA filters (Millipore), and storing the filters in the dark at −20°C until acetone extraction and then analysed fluorometrically (54).
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested after 16 hours and resuspended into 5 mL lysis buffer (200 mM Tris pH 8.0, 500 mM NaCl, 1% (v/v) Triton X-100 (Sigma), 0.02 mg/mL leupeptin ...
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bioRxiv - Plant Biology 2019Quote: ... 150 mM NaCl and 10 mM HEPES pH 7.4 and eluted with 100 μg ml−1 3×FLAG peptide (Sigma).
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bioRxiv - Systems Biology 2019Quote: ... cells were cultured in M9 supplemented with glucose (1X M9 salts, 2 mM MgSO4, 100 µM CaCl2, 0.4% glucose) and 25 μg/mL chloramphenicol (Sigma). In experiment E2 ...
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bioRxiv - Microbiology 2019Quote: ... Phage pellet was resuspended in 2 ml of SM buffer (100 mM NaCl, 8 mM MgSO4, 50 mM Tris pH 7.5 (Sigma) and treated with 0.25 μg of RNAse A (Sigma ...