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Citations for Millipore Sigma :
6601 - 6650 of 10000+ citations for Recombinant Human CYB5A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 500k Day 25 hiPSC-CMs and 100k human Hs27a stromal cells were mixed with 3 U/mL thrombin from bovine plasma (Sigma, T4648) and 5 mg/mL bovine fibrinogen (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Samples of gB-SE or gD-SE immobilized on Strep-Tactin Sepharose beads were incubated with 1 mL of diluted human γ-globulins (G4386; Sigma) (0.082 and 3.04 mg/mL in medium 199 containing 1% FCS and ADCC assay buffer for neutralization assays and ADCC assays ...
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bioRxiv - Microbiology 2022Quote: Human foreskin fibroblast (HFF; ATCC® designation SCRC-1041 ™) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Sigma, D6546) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... the wells were filled with 200 µl fresh M63 media with or without 3 µM of human transferrin (Sigma-Aldrich #T1147) and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... We confirmed the specificity of our IgA ELISA and non-cross reactivity toward IgG using purified human IgG and IgA (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... Cytokine levels in supernatants were analyzed by 38 plex MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore HCYTMAG-60K-PX38) adapted for use with 96-well DropArray plates and DropArray LT210 washing station (Curiox Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... The human embryonic tissues were dissociated for 50 minutes at 37°C with 10 mg/ml Collagenase/Dispase (Sigma Aldrich, 10269638001) in Phosphate Buffered Saline (PBS ...
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bioRxiv - Physiology 2023Quote: Protein lysates were prepared from primary lung endothelial cells and human umbilical vein endothelial cells using ice cold RIPA buffer (Sigma Aldrich) with proteases and phosphatase inhibitor cocktails (Thermo Fisher) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CHO cells were stably transfected with a SNAP-tagged human D2SR (short isoform) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 2 mM glutamine (Sigma-Aldrich, Poole, UK) and supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human CD56 Positive Selection Kit II) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... coating for 5 min at 22 °C protected from light with sterile filtered DOPA in pH 10 [10 mM] Tris buffer DOPA coated PA-gels were washed 2x with sterile PBS and ECM functionalized with 5 µg/mL human fibronectin (Millipore, FC010) in sterile PBS 1 h at 37 °C to generate an expected fibronectin coating density of 6 μM/cm2.
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bioRxiv - Cell Biology 2023Quote: ... Despite the successful reprogramming of a human neonatal foreskin or adult skin (normal) fibroblast line purchased from Sigma (Cat 106-05A), multiple attempts to reprogram the Nigerian fibroblasts failed for reasons that are not entirely clear ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... were superovulated by injecting them with pregnant mare serum gonadotropin (5 IU, Prospec) and human chorionic gonadotropin hormone (5 IU, Sigma-Aldrich) intraperitoneally at 48-h intervals ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C and 5% CO2. Human BLaER1 cells (a kind gift of Thomas Graf) (Rapino et al. 2013) cells were grown in RPMI (Sigma-Aldrich) supplemented with 10% FCS and 2 mM L-glutamine at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: The MISSION® pLKO.1-puro Non-Target shRNA (scr) and the shRNA against the UTR of human ZIP11 gene (5’-TCCTGATTGACTCTGATTATA-3’, Cat. TRCN0000434903) were from Sigma-Aldrich. The mammalian gene expression vector pLV[Exp]-EGFP/Neo-Ef1A (pLV ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.75 IU/kg of human insulin (Actrapid 100 IU/mL, Novo Nordisk) for insulin tolerance tests (ITT) or 2 g/kg pyruvate (Sigma-Aldrich) for pyruvate tolerance tests (PTT ...
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bioRxiv - Immunology 2023Quote: ... 100,000 T84 cells per well were seeded on glass bottom 8-well chamber slides coated with 2.5% human collagen (Sigma #C5533-5MG) diluted in water ...
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bioRxiv - Microbiology 2023Quote: ... Presence or absence of a ZCCHC3 specific band with each clone was determined by western blotting using an anti-human ZCCHC3 antibody (SAB1408147, Sigma-Aldrich).
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bioRxiv - Bioengineering 2023Quote: ... Human mammary epithelial cell line (MCF10A) was cultured in DMEM/F12 (Cytivia: SH30023.01) with 5% horse serum (Sigma Aldrich: H1138-500ML), 0.2% Normocin ...
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bioRxiv - Biophysics 2023Quote: Human Serum from human male AB plasma (H6914, 1st lot SLCH8785, 2nd lot SLCJ3635) and (H4522, lot SLCK9619) were purchased from Sigma-Aldrich. Serum samples from healthy individuals and cancer patients ...
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bioRxiv - Cell Biology 2022Quote: Ibidi µ-Slide I slides (#80106, IbiTreat) were incubated with 10 μg/mL fibronectin (from human plasma, #2006, Sigma-Aldrich Inc.) in PBS at room temperature for 1.5 hr ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and and CD44 (SAB1405590) (mouse) at 1:400 dilution for zebrafish tissue and 1:50 dilution for human tissue (Sigma Aldrich). The following secondary antibodies were used ...
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bioRxiv - Developmental Biology 2023Quote: ... the caudal epididymis was dissected and the epididymal sperm was collected on a needle and capacitated in EmbryoMax Human Tubal Fluid media (HTF, EMD Millipore) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: Serum autoantibodies were quantified using the Luminex xMAP platform (Luminex) with the MILLIPLEX MAP Human Autoimmune Autoantibody Panel (HAIAB-10K, Millipore Sigma) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... purified human neutrophil or mouse BM-derived neutrophils were stimulated with 10-7M N-formyl-Met-Leu-Phe (fMLF; Sigma-Aldrich) or 1 μM WKYMV (Phoenix Pharmaceuticals ...
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bioRxiv - Cell Biology 2023Quote: Round 25mm coverslips were squeaky-cleaned (56) and plasma cleaned before coating with 20µg/mL Human Plasma Fibronectin (EMD Millipore #FC010) at 37C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Systems Biology 2024Quote: The MIA PaCa-2 pancreatic cancer and HEK 293 human embryonic kidney were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma Aldrich, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Bioengineering 2023Quote: ... hydrogel used within the arrays was previously developed to mimic the material properties of human mucus.29 A solution of 4% porcine gastric mucins (PGM; Sigma Aldrich; mucin from porcine stomach ...
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bioRxiv - Genetics 2023Quote: ... Control female mice were injected with 5 U of pregnant mare serum gonadotropin (PMSG, nor272-Prospecbio, Israel) and 46 h later injected with 5 U of human chorionic gonadotropin (HCG, C1063 Sigma-Aldrich) and placed into the home cage of the male to mate overnight ...
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bioRxiv - Immunology 2023Quote: ... and maintained at 37°C with 5% CO2 in complete RPMI in the presence of 5% human AB serum (Sigma-Aldrich) for differentiation into MDMs for 10-14 day (Pierini et al. ...
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bioRxiv - Cell Biology 2023Quote: ... ephrinB1-Fc (R&D, #473-EB) or ephrinB2-Fc (R&D, #496-EB) were pre-clustered using goat anti-human Fc IgG (Sigma, #I2136) in 4:1 ratio for 30min.
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bioRxiv - Microbiology 2024Quote: The human monocytic cell line U937 (ATCC CRL-1593.2) was obtained from ATCC and primary human peripheral monocytic cells (PBMCs) were purchased from Sigma (HUMANPBMC-0002644). U937 cells were cultured in RPMI medium (Genesee ...
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bioRxiv - Microbiology 2024Quote: ... Primary human PBMC were differentiated into macrophages (hMDMs) after culturing for 7 days with RPMI supplemented with 20% heat-inactivated human serum (Sigma-H4522).
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bioRxiv - Cell Biology 2024Quote: ... the media were switched into Phase II erythroid differentiation media consisting of Stem Span SFEMII supplemented with 3% normal human serum (Sigma-Aldrich), 3 IU/ml erythropoietin (R&D Systems ...
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bioRxiv - Immunology 2024Quote: Luminex assays detecting soluble ICAM-1 (sICAM1) were conducted using the MILLIPLEX Human Sepsis Magnetic Bead Panel 1 Kit (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Tween-20 in PBS for 15 min at RT and then incubated with rabbit anti-human RYBP polyclonal antibody (Sigma, HPA053357) and mouse anti-human hCG-β antibody (abcam ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... 5 U thrombin increments were added to microcentrifuge tubes containing 50 µl of human citrated plasma (Sigma Cat No. P9523-1ML), the time to clot formation was recorded ...
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bioRxiv - Bioengineering 2024Quote: To test the micromechanical properties of meniscal cells and newly synthesized PCM, 35-mm petri dish (351008, Life Sciences) was treated with 20 µg/mL human fibronectin (10838039001, Sigma-Aldrich) for 12 h prior to seeding meniscal cells with an initial density of 5,000 cells per dish ...
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bioRxiv - Microbiology 2024Quote: ... by human neutrophils was measured over a time of 30 minutes by monitoring luminol-amplified chemiluminescence using 282 μM luminol (Sigma Aldrich). Neutrophils were stimulated either with negative control (Hank Balanced Salt Solution ...
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bioRxiv - Cell Biology 2024Quote: ... The shRNA plasmids were obtained from the human genome-wide TRC shRNA library (purchased from Sigma-Aldrich, USA, Catalog no. SH0411), available at shRNA resource centre ...
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bioRxiv - Cell Biology 2024Quote: ... For transfection of human or monkey keratinocytes tissue culture plastic vessels were coated with 20 µg/ml rat Collagen I (Sigma, C3867) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... were adjusted to 5 x 106 bacteria per 50 μL in PBS then treated with indicated OspC MAbs (1, 2, 3, 10, 20, 30 μg/mL) in the presence or absence of 20% human complement (Sigma-Aldrich) for 16 or 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were alkylated by addition of 100 μl of 50 mM iodoacetamide (Sigma RPN6320V) prepared fresh in 50 mM NH4HCO3 pH 7.5 buffer and allowed to react in the dark at 20 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... and bounded proteins were eluted with NETN buffer containing 2 mg/ml biotin (Sigma-Aldrich) for 1 h twice at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted kinases (proteins) were concentrated with Amicon Ultra-4 (10K cutoff) spin columns (Millipore), purified by removing the detergent using methanol/chloroform extraction ...