Labshake search
Citations for Millipore Sigma :
6551 - 6600 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany). Unspecific binding on the PVDF membrane was blocked by incubation with the blocking buffer (5% non-fat milk ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were resuspended in 50 mM triethylammonium bicarbonate (TEAB, Sigma cat. no. T7408), reduced for 1 hour at 55°C using 10 mM tris(2-carboxyethyl)phosphine (TCEP ...
-
bioRxiv - Neuroscience 2023Quote: ... Residual protein fragments were precipitated with trichloroacetic acid (Sigma-Aldrich, cat. no. T6399). The supernatant ...
-
bioRxiv - Microbiology 2022Quote: ... YifLStrep protein was eluted with wash buffer containing 2.5 mM d-Desthiobiotin (Sigma).
-
bioRxiv - Cancer Biology 2024Quote: ... followed by protein detection using either Immobilon Western HRP Substrate (WBKLS0500, Geyer/Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
-
bioRxiv - Neuroscience 2022Quote: ... and mouse anti-glial fibrillary acidic protein (GFAP; 1:500, #G3893; Sigma-Aldrich). The next day sections were washed 3 times with the blocking buffer (15 min each ...
-
bioRxiv - Neuroscience 2022Quote: ... The expression of neuronal marker protein NeuN (anti-NeuN, 1:200, MAB377, Millipore) was determined to identify neuronal tdTomato expression.
-
bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using a Quantipro BCA assay kit (Sigma-Aldrich, USA). Protein lysates were prepared in a mixture of RIPA and Lamelli Buffer to produce a final protein concentration of 5 ug/ul ...
-
bioRxiv - Molecular Biology 2022Quote: ... The resolved protein samples were transferred onto Immobilon-P PVDF Membrane (Millipore Sigma), and the blots incubated in the presence of primary antibodies (provided in table S1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Equal loading of protein samples was confirmed via Ponceau S staining (Sigma-Aldrich) for 4 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge at 4 °C ...
-
McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Proteins were analyzed by SDS-PAGE and transferred onto PVDF membrane (Merck Millipore). The membranes were washed with PBS 1x containing 0.1% Tween-20 (PBS-T ...
-
bioRxiv - Cell Biology 2022Quote: ... Eluted protein fractions were pooled and concentrated using Amicon 30K Centrifugal filters (Millipore). The concentrated ...
-
bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing protein were concentrated using Amicon Ultracel-10K/ Ultracel-30K (Millipore) centrifuge filters and subsequently applied to size exclusion chromatography (SEC ...
-
bioRxiv - Neuroscience 2022Quote: ... 10 μg protein was combined with 1 μL 10% Triton X-100 (Sigma) and brought to a volume of 33 μL in Vance buffer (225 mM D-mannitol ...
-
bioRxiv - Bioengineering 2022Quote: ... 1 mM purified protein was mixed with 25% ammonium persulfate (APS) (Sigma-Aldrich) and 6.67 mM [Ru(II)(bpy)3]2+ (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... The expressed GST-fusion protein was absorbed to glutathione-Sepharose 4B (Sigma-Aldrich) followed by extensive washing with phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2024Quote: ... Fractions containing the protein of interest were concentrated (Amicon Ultracel 30 K, Millipore) and further purified by size-exclusion chromatography (SEC ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rabbit anti-zinc transporter 3 (ZnT3 ...
-
bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
-
bioRxiv - Biochemistry 2024Quote: ... the protein was concentrated in a centrifugal filter (Amicon Ultra-15, 10K, Millipore) and washed twice with 15 ml of PBS to remove the imidazole ...
-
bioRxiv - Biochemistry 2023Quote: ... The cell lysate was pre-cleared with Protein A agarose beads (Millipore, Sigma), and 700 μg of the pre-cleared lysate was incubated with 2 μg of either anti-CEP41 ...
-
bioRxiv - Immunology 2024Quote: ... the proteins were transferred to polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). The PVDF membranes were then incubated with a buffer of 5% nonfat dry milk for 2 hours at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... The cell lysate was pre-cleared with Protein A agarose beads (Millipore, Sigma), and 700 μg of the pre-cleared lysate was incubated with 2 μg of either anti-CEP41 ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins of interest were detected with anti-FLAG antibody (Sigma F1805, 1:1000) and anti-PCNA antibody (Santa Cruz sc-8349 ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with addition of 1 µL of 500 mM dithiothreitol (Sigma) at 25°C for 1 h and alkylated with 3 µL of 500 mM iodoacetamide for 1 h at room temperature in the dark and quenched with 2 µL of dithiothreitol for 10 min ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein samples were incubated with 0.2mM methoxypolyethyleneglycol maleimide (NEM-PEG, 5kDa; Sigma-Aldrich) for 1 minute on ice ...
-
bioRxiv - Biophysics 2024Quote: ... The dialyzed sample was filtered with a low protein binding filter (Millipore Sigma) and run through a SourceQ15 anion exchange column (Cytiva) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The lysates were pre-cleared using Protein A agarose beads (11134515001, Sigma-Aldrich) at 4 ℃ for 2 h ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich), and equal amounts of protein were analyzed by western blot.
-
bioRxiv - Cell Biology 2024Quote: ... The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich) and centrifuged (12 000 g ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were electrophoretically transferred overnight on to PVDF membrane (03010040001, Roche, Sigma-Aldrich). The membrane was blocked in 5% non-fat dry milk in TBS with 0.05% Tween 20 (TBST ...
-
bioRxiv - Microbiology 2024Quote: ... External mass calibration was performed thanks to SpheriCal Aqua Protein Low kit (Sigma).
-
bioRxiv - Immunology 2024Quote: ... Eluted proteins were buffer exchanged using 50 kDa Amicon centrifugal units (EMD Millipore), resuspended in phosphate-buffered saline (PBS) ...
-
bioRxiv - Molecular Biology 2024Quote: Proteins were transferred from SDS PAGE onto Immobilon-FL PVDF membranes (Millipore Sigma) using a semi-dry transfer apparatus (15V ...
-
bioRxiv - Neuroscience 2024Quote: ... CDK5 was immunoprecipitated (4°C overnight with 2x Protein A agarose (Sigma-Aldrich)) from SCN samples at ZT14 in the dark or after a light pulse (LP. ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins in the supernatant and pellet were solubilized in 1XLDS sample buffer (Sigma) and analyzed by SDS-PAGE and western blots with anti-CAPS and anti-alpha-tubulin antibodies.
-
bioRxiv - Molecular Biology 2024Quote: ... The collected proteins were concentrated by an Amicon Ultra-3k centrifugal filter (Millipore), exchanged with PBS supplemented with 100 mM NaCl and 50% glycerol ...
-
bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...
-
bioRxiv - Neuroscience 2024Quote: ... the proteins were incubated with streptavidin-agarose (16-126; Millipore sigma, Burlington, MA) for one night at 4 °C ...
-
bioRxiv - Microbiology 2024Quote: ... The protein was concentrated using a 10 kDa MWCO Amicon concentrator device (Millipore). The purified proteins and protein standards were subjected to a colorimetric bicinchoninic acid (BCA ...
-
bioRxiv - Cell Biology 2024Quote: ... goat anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000; Sigma-Aldrich, catalogue# SAB2500462 ...
-
bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore, Burlington, MA, USA). Incubation with primary antibodies was carried out at 25 °C for 1h ...
-
bioRxiv - Immunology 2024Quote: ... The proteins were transferred from SDS-PAGE to PVDF membrane (0.2-μm, Millipore) in Tris-Glycine Transfer buffer (Bio-Rad ...
-
bioRxiv - Immunology 2024Quote: ... The supernatant was passed through low-protein binding 0.45-µm syringe filter (Millipore). The lentivirus was purified by two different methods for comparison ...
-
bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using Amicon™ Ultra centrifugal filter units (Millipore Sigma) with a molecular cutoff of 10 kDa and stored at −80 °C.
-
bioRxiv - Microbiology 2024Quote: ... The refolded proteins were concentrated with Amicon 3 kDa Ultracel filters (EMD Millipore). For GFP-PSB15 purification ...
-
bioRxiv - Microbiology 2024Quote: ... The protein-containing cell culture supernatant was filtered through 0.22μm PES membranes (Millipore) and passed over 0.5ml of a Strep-Tactin Superflow (IBA Lifesciences ...
-
bioRxiv - Microbiology 2023Quote: ... G9R and A1L proteins were labeled with primary anti-Flag (F1804, Sigma-Aldrich) and anti-HA (26183 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins in the supernatant were digested with 100 µg/mL proteinase K (Sigma) at 37°C overnight ...