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Citations for Millipore Sigma :
6451 - 6500 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... denatured proteins were loaded onto 30 kDa amicon filter (Merck Millipore, Darmstadt, Germany). The buffer was exchanged with UA solution (8 M UREA in 0.1 M TEAB pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... imidazole was removed and the protein was concentrated using a Centricon (Merck Millipore) and preserved at −80 °C in small fractions at 2 mM concentration.
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bioRxiv - Neuroscience 2020Quote: ... Proteins separated in the gel were transferred to a PVDF membrane (Merck Millipore) using a submerged blotting apparatus and then visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Biophysics 2020Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37 °C in LB medium containing 50 μg ml−1 kanamycin and 34 μg ml−1 chloramphenicol till an OD600 nm of 0.7 was reached ...
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bioRxiv - Cancer Biology 2021Quote: ... Co-Immunoprecipitation was performed using PureProteome™ Protein A Magnetic Beads (Millipore, LSKMAGA10). Protein extracts were first precleared using 10μl of beads for 200μg of proteins (1-hour incubation at 4°C) ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein was also visualized using 4-Chloro-1-naphthol solution (Sigma Aldrich) which is a substrate for HRP in a reaction that results in a colored precipitate.
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bioRxiv - Plant Biology 2021Quote: ... GST-PPKs were tested against 5 µg myelin basic protein (MBP; Sigma Aldrich) and 5 µg Casein in 0.1 M Tris pH 8.8 (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rat anti-mCherry (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... resolved proteins were further transferred to a PVDF membrane (0.45 μm; Millipore, USA) for immunoblotting and the blots were blocked using Starting Block T20 (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using a 50-kDa cutoff Amicon filter (Sigma-Aldrich) and purified on a Superose 6 Increase 10/30 gel filtration column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was extracted using 100 µL of lysis buffer (7 M urea (Sigma), 2 M thiourea (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore, Burlington, MA) in transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... The immunocomplex was captured by adding Protein G Agarose (Merck Millipore, Darmstadt, Germany). The protein binding to the beads was boiled in 2 x Laemmli sample buffer (Bio–Rad ...
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bioRxiv - Genetics 2022Quote: ... The protein solution was loaded onto glass slides within a silicone isolator (Sigma) and imaged using a Zeiss LSM 800 ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were transferred onto a PVDF membrane (Immobilon®-P Membrane, Millipore #IPVH00010) (120mA/gel ...
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bioRxiv - Immunology 2022Quote: ... protein bands were stained with silver nitrate (ProteoSilver Silver Stain Kit, Sigma-Aldrich). The two recombinant proteins were analyzed in non-denaturing conditions on a Superose 6 10/300 GL size exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2022Quote: ... and the proteins were detected with the following antibodies: Flag (Sigma-Aldrich; F7425), GFP (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... for 8 h with protein A/G agarose beads (Sigma-Aldrich, Burlington, Massachusetts) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10μg of antibody was coupled to 50μl packed Protein A beads (Sigma P9424) by incubation in 1ml PBS (0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated using a 3 kDa cutoff spin concentrator (Millipore Sigma UFC9003) to a volume of 2 mL ...
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bioRxiv - Plant Biology 2022Quote: ... 50 µg GST–CAR9 protein emulsified with Incomplete Freund’s adjuvant (F5506, Sigma–Aldrich) was injected into the ICR mouse and then once again in the next week ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-glial fibrially acidic protein (GFAP, 1:100, MA360, Millipore, MA, USA) and rabbit anti-phosphorylated TrkBS478 (1:200 R-1718-50 ...
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bioRxiv - Biochemistry 2019Quote: ... The purified protein was concentrated with an Amicon Ultra-10 (Millipore, Billerica, MA) for future NMR study ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and Spo11 was immunoprecipitated from total soluble protein using anti-FLAG antibody (Sigma) and protein-G-agarose (Roche) ...
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bioRxiv - Biophysics 2019Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37°C in LB medium containing 50μa.g ml-1 kanamycin and 34μg ml-1 chloramphenicol till an ODóOOnm of 0.7 was reached ...
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bioRxiv - Molecular Biology 2019Quote: ... plasmids for different Cas9 proteins were expressed in Escherichia coli Rosetta2 (DE3) (Novagen). The protein expressing Rosetta2 (DE3 ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were then transferred to an Immobilon-FL PVDF membrane (Millipore, Billerica, MA) on ice either for 2 h at 100 V ...
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bioRxiv - Neuroscience 2019Quote: ... the protein was transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-P; Millipore). The membrane was blocked with 5% skimmed milk and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated protein/DNA complexes were prepared using the Magna ChIP A kit (Millipore) following manufacture’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were extracted by homogenizing the tissue in ice-cold RIPA buffer (Sigma) and lysates were centrifuged at 16,000 × g at 4°C ...
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bioRxiv - Pathology 2019Quote: ... Co-IP assays were performed by incubating total proteins with anti-HA (Sigma) agarose followed by column wash and elution according to manufacturer’s instructions (Pierce ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The equal loading of the blotted protein was verified by Ponceau S (Sigma) staining ...
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bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
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bioRxiv - Cell Biology 2019Quote: ... after which the proteins were transferred to a PVDF membrane (Immobilon-P, Millipore). Primary antibodies were incubated overnight at 4 °C in 1% milk ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing the purified proteins were concentrated using Amicon Ultra concentrators (Millipore Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Total protein concentration in CFE was determined by Bradford reagent (Sigma-Aldrich, USA), and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... The protein concentration in the extract was determined with a Bradford assay (Sigma) and used for normalisation of the SOD activity.
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bioRxiv - Cell Biology 2020Quote: ... Protein A or G agarose (Pierce, 20333 or Millipore-Sigma 16-201, respectively) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A or G agarose (Pierce, 20333 or Millipore-Sigma 16-201, respectively) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were detected with the primary antibodies against Flag (1:1000, Sigma F7425), GFP (1:1000 ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins were precipitated with 1 μg of anti-Flag antibody (F-3165, Sigma) for 2 h at 4°C followed by the addition of 15 μl protein G-Sepharose beads for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... The total nuclear protein was lysed with CHAPS buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Plant Biology 2021Quote: ... Sonicated samples were first precleared with Protein A Agarose beads (Millipore #16-157) and then mixed with antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... antiserum against FLAG fusion proteins was obtained from Sigma-Aldrich (F1804, mouse monoclonal). An anti-rabbit horseradish peroxidase-conjugated antiserum (#7074 ...
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bioRxiv - Microbiology 2020Quote: ... The protein-DNA complex was treated with 1 U/μl DNase I (Novagen) to digest non-specific DNA ...
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bioRxiv - Immunology 2021Quote: ... Protein bands were revealed by enhanced chemiluminescence reagents (ECL™ Prime, Merck Millipore) in a ChemiDoc XRS+ imaging system (BioRad ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was precipitated by adding 1 volume of TCA (trichloroacetic acid, Sigma Aldrich) to 4 volumes of supernatant ...
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bioRxiv - Microbiology 2020Quote: ... protein containing fractions were pooled and concentrated with an Amicon Ultracel-10K (Millipore) according to the experimental requirements.
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bioRxiv - Microbiology 2020Quote: ... Proteins in the supernatants were precipitated with 4x volume cold acetone (V800023, Sigma) overnight at −20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... or proinsulin Ab (CCI-17) bound protein G beads (GE17-0618-01, Sigma) for overnight at 4°C ...