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Citations for Millipore Sigma :
601 - 650 of 7563 citations for ssc mir 323 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Thereafter, the cells were washed twice with washing buffer (25% formamide, 2X SSC) containing DAPI (1 mg/mL, Sigma Aldrich), for 30 minutes at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed ovaries were washed twice in PBST for 10 minutes and incubated with a final probe concentration of 1nM/probe in 250μL HYBEC solution (2x SSC, 15% ethylene carbonate (Sigma Aldrich), 1mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... 16806-25] in 10 mM Tris-HCl [pH 8.0], 2× saline sodium citrate [SSC], glucose oxidase [37 µg/mL, Sigma-Aldrich, G2133-10KU] ...
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bioRxiv - Neuroscience 2022Quote: ... the sample was washed 4 times with 2X SSC and fluorophores were cleaved off by reducing the thiol bond with 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma) in 2X SSC during two washes of 10 minutes at 22 °C ...
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bioRxiv - Developmental Biology 2021Quote: Tissues were moved to GLOX buffer (0.4% Glucose, 1% Tris, and 10% SSC) containing 50 ng/ml DAPI (Sigma, D9542) for 10 min followed by GLOX buffer wash ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were washed with blocking buffer (4X-SSC/0.1% Triton X-100/4% BSA (Bovine serum albumin; Sigma, catalog #A7906)) at RT for 30 mins ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were washed in containers containing large volumes and under agitation (400 rpm) as following: 100 mL of preheated buffer 1 (2X SSC and 0.1X SDS (Sigma 71736); 50°C ...
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bioRxiv - Neuroscience 2023Quote: ... the dyes were removed by flowing 2.5 mL of cleavage buffer comprising 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; Sigma, 646547) with 15 min incubation in the flow chamber to cleave the dyes linked to the readout probes through disulfide bond ...
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bioRxiv - Neuroscience 2023Quote: ... the fluorescent dyes were removed by flowing 2 mL of the cleavage buffer containing 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; 646547, Sigma) with a 15-min incubation time in the flow chamber to cleave the disulfide bond linking the dyes to the readout probes ...
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bioRxiv - Cancer Biology 2020Quote: ... Membrane-immobilized protein detection used ECL Western Blotting Detection Reagents (GE Healthcare RPN2106; Millipore Sigma GERPN2106).
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bioRxiv - Cancer Biology 2021Quote: All cell lines were verified to be mycoplasma negative using the Venor GenM Mycoplasma Detection kit (Sigma Aldrich). 4×106 cells (WSU-DLCL2 WT ...
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bioRxiv - Molecular Biology 2021Quote: PLA was performed using DuoLink red detection kit and DuoLink anti-mouse and anti-rabbit antibodies (Millipore-Sigma Cat ...
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bioRxiv - Developmental Biology 2019Quote: ... TUNEL staining was performed based on the instruction of the ApopTag Red in situ detection kit (Millipore S7165). Antibodies were used at the following final concentrations ...
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bioRxiv - Microbiology 2021Quote: Duolink PLA assay was performed according to the kit manual (Duolink In Situ Detection Reagents Red, DUO92008, Sigma). In brief ...
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bioRxiv - Pathology 2021Quote: ... TUNEL assay was carried out on cryosections (15μm thickness) using Apoptag Peroxidase In Situ Apoptosis Detection Kit (Millipore) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were tested for mycoplasma contamination by means of Venor GeM Mycoplasma Detection Kit (MP0025-1KT, Sigma Aldrich) and grown in M199 complete medium ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Lactate dehydrogenase activity in the basolateral medium was assayed using the Cytotoxicity Detection Kit (Sigma, cat. no. 4744934001). For the high control ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were routinely tested for mycoplasma contamination using LookOut Mycoplasma qPCR Detection Kit (Sigma, MP0035-1KT).
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bioRxiv - Developmental Biology 2019Quote: ... Cell death was measured by TUNEL assay using the ApopTag® Plus Fluorescein In Situ Detection kit (Millipore). Fluorescently stained sections were imaged on a Zeiss LSM710 confocal microscope.
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bioRxiv - Cell Biology 2020Quote: TUNEL staining was carried out by using an ApopTag® Fluorescein in Situ Apoptosis Detection Kit (S7110, Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: Oxidized proteins containing carbonyl groups were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma-Aldrich: cat#S7150). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... we cultured T-SLICE in the presence of 100 μM of the EF5 Hypoxia Detection Kit (Sigma-Aldrich). Cell culture media was supplemented with 100 μM EF5 incorporated into the media for 6 ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The HEK cells used in this study were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). These cells were cotransfected with 0.1 - 1 μg of mutant hHV1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr ...
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bioRxiv - Cell Biology 2023Quote: ... iNs were subjected to in situ PLA using Duolink In Situ Detection Reagents Red kit (Sigma, catalog #: DUO92008) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... allowing for detection of the secondary HRP antibody detected using a chemiluminescence peroxidase substrate kit (Sigma, #CPS-1) and subsequent detection of the GAPDH loading control detected using its laser based infrared detection system ...
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bioRxiv - Neuroscience 2023Quote: ... TUNEL-positive cells were stained using the in-situ cell death detection kit TMR red (11684795910; Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... and detection reagent Orange (Sigma-Aldrich) for 100 min at 37°C ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... Superoxide detection by cytochrome C (Sigma) was performed as previously described (Nguyen et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 2.0 Protein Detection System (Millipore/Sigma) for 10 min and incubated with monoclonal anti-C-cadherin antibody ...
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bioRxiv - Neuroscience 2023Quote: ... 2.0 Protein Detection System (Millipore/Sigma) for 10 min and incubated with monoclonal anti-C-cadherin antibody ...
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bioRxiv - Cell Biology 2020Quote: ... are stained for 5 minutes at room temperature RT with 0.5μl of PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling (#P7333, Sigma) and then polished with the exosome spin columns (#4484449 ...
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bioRxiv - Microbiology 2019Quote: ... contained in the Kap2G Robust PCR kit (Sigma Catalogue No. KK5005) per sample and 11 µL added to each well need to be used in a 96-well plate ...
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bioRxiv - Genomics 2022Quote: ... contained in the Kap2G Robust PCR kit (Sigma Catalogue No. KK5005) per sample and 11 µL added to each well need to be used in a 96-well plate ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification was performed using KOD Hotstart polymerase kit (EMD Millipore) and primers flanking the gRNA target sites (Table 2) ...
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bioRxiv - Microbiology 2023Quote: ... then purified using GenElute PCR Clean-Up Kit (Sigma, SKU NA1020), and KAPA Pure Beads (Roche-07983298001) ...
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bioRxiv - Neuroscience 2021Quote: ... 350μl of RNAzol RT (Sigma-Aldrich) were added to the samples and lysis was performed with TissueLyser II (Qiagen ...
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bioRxiv - Genetics 2019Quote: ... 50 uL of PCR product of each sample was purified with SpinPrep PCR Clean-Up Kit (EMD Millipore Corp) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... the tagmented DNA was amplified by PCR using the KAPA2G Robust PCR kit (Sigma-Aldrich, St. Louis, MO, USA) using the GC buffer along with 0.2 μM of each custom P5 and P7 indexing primers (File S1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Template plasmid was digested using DpnI and the PCR products were purified using a PCR purification kit (Sigma-Aldrich). Subsequently ...
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bioRxiv - Systems Biology 2021Quote: ... and miR-290-295_KO cluster mESC lines (129/Ola background) were cultured in Dulbecco’s Modified Eagle Media (DMEM) (Sigma-Aldrich), containing 15% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Eighteen microliters of high-titer lentivirus expressing miR-382 mixed with 2 μL 0.2% Evans blue dye (Sigma-Aldrich) in PBS was injected in the third mammary gland using a 34-gauge needle (Hamilton) ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos are rinsed in HB-buffer (HB+ without torula RNA and heparin) supplemented with saline-sodium citrate buffer (SSC, Sigma, S6639) and then transferred gradually to PBT ...
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bioRxiv - Genomics 2020Quote: ... The complete reaction mix was again removed and a slide wash with one 10 minute wash of the wells with 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC was performed ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS was incubated in each well for 5 minutes and cells were washed with 2x SSCT (2x SSC with 0.1% TWEEN® 20 (P9416-100ML; Sigma-Aldrich). For storage ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was then neutralized with SSC and hybridized with a DIG-labeled telomere probe prepared as described 48 in DIG Easy Hyb (Sigma-Aldrich) solution overnight at 42°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were thermally aged overnight at 37 °C and then 60 min at 60 °C, followed by treatments with RNase A (200 µg/mL in 2× SSC, 60–90 min, 37 °C) (Sigma-Aldrich) and then pepsin (50 µg/mL in 10 mM HCl ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were washed with wash buffer (10% formamide in 1X SSC and 0.1% Tween-20) for 5 min, then incubated with the hybridization mix (10% formamide, 1X SSC, 10% Dextran sulfate (w/v) (Sigma, D8906), 100nM each in situ probe ...
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bioRxiv - Genomics 2022Quote: ... before slides were detached from their hybridization chambers and washed for 10min at 50°C in 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC ...