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Citations for Millipore Sigma :
601 - 650 of 662 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing AR9 nvRNAP holoenzyme or its mutants were pooled and concentrated (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane, EMD Millipore) to a final concentration of 3 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... or 1 mg of protein extract (for CSR-1 IPs comparing WT IP with catalytic mutant) was incubated with 15 µl of packed Anti-FLAG M2 Magnetic Agarose Beads (Sigma M8823) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Biofilm matrix composition was investigated through spotting 10 μL of each mutant (representing 105 CFU) on agar supplemented with 40 μg/mL Congo red (Sigma, C6277) to examine curli production ...
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bioRxiv - Cell Biology 2022Quote: Late L4–young adult mlt-4:RFP mutants (GUN405) were mutagenized for 4 hr at 22 °C in 0.5 mM N-nitroso-N-ethylurea (ENU, Sigma Aldrich N3385). Worms were washed in M9 buffer (22 mM KH2HPO4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mitral valves were dissected from control and mutant hearts at E18.5 and were digested with 1mg/ml collagenase and dispase (Sigma-Aldrich, #10269638001) at 37°C for 4 minutes followed by 0.25% trypsin at 37°C for 10 minutes to acquire single cell suspensions ...
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bioRxiv - Genetics 2019Quote: Total RNA was isolated from healthy wandering third instar larvae of wild type and delayed third instar larvae from homozygous l(3)tb mutant using TRIzol reagent following the manufacturer’s recommended protocol (Sigma-Aldrich, India). The samples were incubated with 2U of RNase-free DNaseI (MBI fermentas ...
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bioRxiv - Genetics 2019Quote: Adult zebrafish from both eef1a2 mutant lines and age-matched wild-type controls were fixed in 10% neutral buffered formalin (Sigma-Aldrich). Spinal cord sections were cut at a thickness of 3 µM ...
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bioRxiv - Microbiology 2021Quote: Streptomyces scabiei 87-22 and its ΔcebR mutant (22) were routinely cultured in Tryptic Soy broth (TSB, 30 g/l, Sigma-Aldrich) or ISP2 (for 1l ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitations of 3XF-PPIA and 3XF-Mutant PPIA transiently transfected cells were performed with a mouse monoclonal anti-FLAG antibody (clone M2, Millipore Sigma) in 293T cells ...
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bioRxiv - Bioengineering 2021Quote: ... PduN point mutants at glycine 52 (G52) were generated using QuikChange site-directed mutagenesis with KOD Hot Start DNA polymerase (Sigma Aldrich) on a PduN sequence ...
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bioRxiv - Microbiology 2020Quote: ... and mutants (lpg1-/- lpg5A-/lpg5B-) were lysed in 1% NP-40 in the presence of an protease inhibitor cocktail (Sigma, 11836170001). Equal cell equivalents were separated on a 10% SDS PAGE (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... Apparent steady-state kinetic constants were determined by monitoring the WT and mutant EPSPS activities at varying concentrations of 2-phosphoenolpyruvate (PEP – Sigma-Aldrich), and fixed-saturating concentrations of shikimate-3-phosphate (S3P - Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: The C85A/L194A and C85A/L194A/H234C mutant CLC-ec1 protein crystals were grown in 29% (w/v) PEG400 (Sigma-Aldrich), 200 mM NaCl (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... Screening of Arabidopsis mutants was performed with seedlings growing under standard media and transferred to plates containing 140 mM NaCl (Sigma-Aldrich) or 300 mM mannitol (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2020Quote: Mammalian expression vectors encoding Flag-epitope tagged full-length Csk and eGFP-tagged Ptpn18 WT & Y/F mutant were transfected into 293T cells using 5 ug/mL PEI (Polyethylenimine, Sigma Aldrich) in Opti-MEM (GIBCO) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells and WT or mutant A1R expressing HEK 293 cells were harvested using a non-enzymatic cell dissociation solution (Sigma-Aldrich) and washed with PBS prior to counting ...
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bioRxiv - Cell Biology 2020Quote: ... All other mutants described in this report were generated by site-directed mutagenesis using oligonucleotide primers synthesised by Sigma (Poole, UK). The validity of all constructs was confirmed by sequencing (Dundee DNA sequencing Service ...
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bioRxiv - Plant Biology 2019Quote: ... DNA was extracted from wild type Ps LMG5084 and its PL1-insensitive mutants using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich). Libraries were prepared with the NEBNext Ultra II library kit according to the manufacturer’s instructions and sequenced on the Illumina HiSeq X platform to obtain 150 bp paired end reads with an average depth of 40-fold ...
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bioRxiv - Biochemistry 2021Quote: The wildtype and mutant forms of the aqMutL NTD (5 μM) were reacted with 30 ng/μl trypsin (from bovine pancreas; Sigma Aldrich) in a buffer consisting of 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Physiology 2022Quote: The ovarian tissues of the mutants and WT control were homogenized and prepared by RIPA lysis buffer (EMD Millipore, Billerica, MA). The supernatant was collected ...
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bioRxiv - Plant Biology 2023Quote: ... All ATG3 recombinant proteins (wild-type and Cys-to-Ser mutants) were purified by affinity chromatography on a His-Select Nickel Affinity Gel (Sigma, P6611) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the WT and ΔfakA mutant were grown with fatty acid-containing and fatty acid-free HSA (Sigma-Aldrich, St. Louis, MO) at 10-40 mg/mL in TSB ...
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bioRxiv - Microbiology 2023Quote: ... When necessary, culture media was supplemented with appropriate antibiotics (chloramphenicol 10 μg/mL, erythromycin 10 μg/mL (Sigma-Aldrich, deletion mutants) or erythromycin 25 μg/mL (NTML mutants) ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal deletion mutants were verified by amplification of the genomic region from isolated genomic DNA (GeneElute Bacterial Genomic DNA Kit, Sigma Aldrich) and by whole genome sequencing (MicrobesNG/Plasmidsauraus) ...
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bioRxiv - Biophysics 2023Quote: ... Aggregation of mutant was carried out with a protein concentration of 100 µM in 10 mM phosphate buffer (Sigma-Aldrich, USA), pH 7.4 at 37ºC without agitation.
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bioRxiv - Developmental Biology 2022Quote: ... The progeny of stat3 heterozygous mutant incrosses using the TgBAC(neurod1:EGFP) background were treated with 50 μM DCHC (Sigma, D5569) from 24 to 48 hpf ...
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bioRxiv - Molecular Biology 2023Quote: The dox-inducible A375P cells were treated with doxycycline to express MITF WT and MITF mutant proteins for 24 hours and then treated with 40 µg/ml cycloheximide (Sigma #66819), in presence or absence of 200nM TPA ...
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bioRxiv - Synthetic Biology 2023Quote: The segregation analysis was performed on genomic DNA extracts of Synechocystis and Synechocystis mutants obtained using the Gen-Elute Bacterial Genomic DNA Kit (Sigma-Aldrich) by effectuating a screen for GGR and ShMKS incorporation into slr0168 using PCR ...
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bioRxiv - Genetics 2023Quote: ... Experimental Villin-CreERT2 Hnf4 mutant mice (8–12 weeks old) were intraperitoneally administered tamoxifen at 50 mg/kg/day (Sigma, T5648). Wild-type ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length BTK and BTK linker kinase domain (WT and mutants) were produced by co-expressing with YopH in BL21(DE3) (Millipore Sigma) or BL21-Gold(DE3 ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid-encoded wild-type and mutant rRNA overexpression (Figure 2B) was induced by adding 12 mM sodium propionate (Sigma-Aldrich #P1880) to bacterial cultures at OD600~0.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... transformed with HsaBR (or its mutant HsaBR R340E) was inoculated in 25 ml of LB media in presence of 100 μg/ml ampicillin (Millipore, 171254-25GM) and grown overnight at 37°C with mild shaking of 225 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... transformed with YenB (or its mutant YenB S31A/Y100F) was inoculated in 25 ml of LB media in presence of 100 μg/ml ampicillin (Millipore, 171254-25GM) and grown overnight at 37 °C with mild shaking of 225 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... transformed with PltB (or its mutant-PltB S35A) was inoculated in 25 ml of LB media in presence of 50 μg/ml kanamycin (Sigma, K1377-5G) and grown overnight at 37 °C with mild shaking of 225 rpm ...
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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bioRxiv - Neuroscience 2020Quote: Enucleations were performed on 13 dpf wildtype and tmt-opsin1b mutant juvenile fish upon anaesthetization with 0.03% tricaine (MS-222) (Sigma, St. Louis, MO). After the surgery ...
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bioRxiv - Cell Biology 2020Quote: ... for wild type or mutant BAG3-GFP and fixed in ice-cold methanol (67-65-1, Sigma-Aldrich, Saint Louis, MI, USA) for 20 minutes ...
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bioRxiv - Plant Biology 2019Quote: ... were sourced from Nottingham Arabidopsis Stock Centre and homozygous mutants where confirmed through genotyping PCR on genomic DNA using REDTaq® ReadyMix™ (Sigma) following their protocol ...
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bioRxiv - Genetics 2020Quote: Wildtype and mutant COCH-expressing FLp-inTM T-RExTM 293 cells were cultured in DMEM-AQ medium (Sigma Aldrich, Saint Louis, USA) supplemented with 10% Fetal Calf Serum ...
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bioRxiv - Systems Biology 2022Quote: The indicated yeast mutants were cultivated in a 96-well format in SD media (5 g/L ammonium sulfate (Sigma-Aldrich: A4418), 1.7 g/L Yeast Nitrogen base (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used for immunostaining: monoclonal mouse anti-mutant huntingtin (1/3000; PHP2(34)) and polyclonal guinea pig anti-NEUN (1/1000; Millipore, No. ABN90P). All staining was performed with floating sections ...
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bioRxiv - Microbiology 2023Quote: ... The ligated fragment was transformed into the first-step constructed mutant strain and selected by using plates containing p-Cl-phe (4 mg/mL, Sigma, United States). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated from overnight cultures of the wild type and mutant strains using GenElute Bacterial Genomic DNA Kits (Sigma-Aldrich, USA). Library preparation and whole genome sequencing were performed by Glasgow Polyomics ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of Cypridina noctiluca luciferase and Omicron RBD or its mutants were co-transfected (9:1) into HEK293 cells using X-tremeGENE 9 (Millipore-Sigma XTG9-RO). After 16 hours ...
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bioRxiv - Biochemistry 2023Quote: ... 500 nM GBP2FL or 300 nM GBP2GD protein (wild type or mutant) was mixed with 200 µM GTP (Sigma, Cat No. G8877) in a reaction buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: The chvI and mirA coding sequences (wild type and mutant alleles) were amplified from cloning constructs (primer sequences in Table 2) and ligated into pET-28a(+) (Novagen/ EMB Biosciences/ Millipore). Protein-expression plasmids were transformed into E.coli Top10 F’ for collection and sequence verification and then transformed into E.coli BL21/λDE3 for expression and protein purification ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were infected with APOBEC3A wild-type or catalytically inactive mutant APOBEC3AE72A viral particles for 24 hours at 37°C with 8 μg/mL of polybrene (Millipore #TR-1003-G). PC9 cells were selected in 600 μg /mL of G418 (Gibco #10131035 ...
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bioRxiv - Microbiology 2020Quote: ... The isogenic mutant 3B1::Tn20 is chloramphenicol-resistant and was grown on media additionally supplemented with 20 μg/ml chloramphenicol (Sigma, St. Louis, MO). H ...
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bioRxiv - Biophysics 2019Quote: A final concentration of 20 µM AS (WT or mutant) was obtained in 20 mM ammonium acetate (Sigma Aldrich, St. Louis, MO, USA) pH 7 and measured as a control ...
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bioRxiv - Genetics 2020Quote: Total RNA of the original #326 (wildtype) and mutants were isolated from vegetative mycelia and hyphal knots using TRI reagent® (Sigma-Aldrich, USA) following the manufacturer’s instruction with three biological replicates ...