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Citations for Millipore Sigma :
601 - 650 of 764 citations for Cy7 Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 5 μl of an overnight culture of OD600 = 5 suspended in water was spotted onto LB without NaCl agar plates containing the dye Congo red (40 μg/ml, Sigma) and Coomassie Brilliant Blue G-250 (20 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Synchronized feeding third instar female larvae were sorted and placed in a hole created at the centre of an agar plate (3.3% wt/vol) filled with yeast paste containing 4% (w/v) FD&C Blue dye # 1 (Sigma Aldrich). The larvae were allowed to feed in the dyed-yeast paste for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... Unbound dye was removed by repeated dilution and centrifugation through an Amicon® Ultra-15 Centrifugal Filter Unit (Millipore Sigma), prior to size exclusion chromatography ...
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bioRxiv - Developmental Biology 2022Quote: ... Probe sets complementary to cycB (CG3510; 48 oligos) or egfp (32 oligos) were conjugated to Atto 647N dye (Sigma-Aldrich) or Atto 565 dye (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... Pure tubulin and BSA were labeled with commercial NHS-conjugated dyes (Cy5 or Alexa568) according to supplier’s instructions (Sigma: GEPA150101). A similar method was used to conjugate biotin to tubulin (EZ-Link™ NHS-PEG4-Biotin ...
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bioRxiv - Biochemistry 2020Quote: ... Brain slices were mounted on glass slides and incubated for 5 min in the dark with 1μg/mL Hoechst dye (33342 Sigma-Aldrich) in PBS to detect cell nuclei ...
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bioRxiv - Neuroscience 2020Quote: ... was injected into the heart chamber of the fish along with a high molecular weight fluorescent dye (2000 kDa dextran-conjugated FITC (3 mg/ml, Sigma). We estimate that 1 nl of a 10 nM Aβ injection into a ∼3.01 (±0.16 ...
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bioRxiv - Microbiology 2021Quote: ... Labeled lentivirus particles were then purified to eliminate excess free dyes and lipids by ultracentrifugation for one hour at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Microbiology 2019Quote: ... along with the membrane-impermeable nuclear dye Cytotox Green (Essen BioScience Inc. Ann Arbor, MI) and cell-permeable Hoechst stain (Sigma). Cytotoxicity was measured on an ImageXpress Pico (Molecular Devices ...
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bioRxiv - Biophysics 2019Quote: ... Then the sample was rinsed once with deionized water and was incubated with 200 µL 1:106 dye dilution in 0.1 M sodium bicarbonate (792519-500G, Sigma-Aldrich) for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed 3 x 5 min with 3% BSA in PBS-T and incubated with the following DNA dye and secondary antibody dilutions in blocking solution for 3 hours at room temperature: Hoechst 33342 (0.2 mM, Sigma-Aldrich), Goat Anti-Chicken Alexa Fluor® 647 (4 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and the ampicillin media was stained by adding 5 µl and 3 µl of a fluorescent dye (rhodamine, Sigma S1402) in 100ml of media used to grow MG:GT and MG/pBGT cells ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were kept at least 45 min at 33_C in the “recovery” ACSF (similar to cutting ACSF with 2 mM MgSO4 and CaCl2) before incubation with the calcium sensor Cal520AM (AAT biotech) and the glial dye SR101 (Sigma) for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with a fluorometric calcium indicator (Cal520AM, 1:1000, AAT biotech) and glial cell dye (SR101, 1:1000, Sigma) directly in the culture medium for 30min ...
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bioRxiv - Biochemistry 2022Quote: ... and equimolar TCEP for 30 minutes on ice before addition of a 5-fold molar excess of the green fluorescent CF488A maleimide dye (Sigma). The mixture was gently agitated overnight at 4 °C in a 1.5 mL amber tube ...
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bioRxiv - Developmental Biology 2022Quote: ... implantation sites were identified by intravenous injection of 0.1 ml of 1% Chicago blue dye (Sigma-Aldrich, St. Louis, MO) dissolved in saline.
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bioRxiv - Immunology 2022Quote: ... Effector (TILs) and target cells (autologous tumor cell lines) were labeled respectively with 1μM PKH67 and PKH26 fluorescent dyes (Sigma-Aldrich) according to the manufacturer’s protocol and loaded on the chip array ...
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bioRxiv - Synthetic Biology 2022Quote: ... then mixed with 1 mL of the reactive dye 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF) (Sigma Aldrich), previously dissolved in DMSO (20 mg/mL) ...
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bioRxiv - Biochemistry 2022Quote: ... Alexa FluorTM 555 NHS stock solution was prepared immediately before use by solubilizing the dye in DMSO (Sigma-Aldrich, >99.9 %) to stock concentration of 1.6 mM ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Neuroscience 2023Quote: 1×109 particles/ml EMVs and EEXs were pre-incubated with PKH 26 dye (2 μM, Sigma, USA, cat# PKH26GL) [57] ...
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bioRxiv - Physiology 2023Quote: ... Samples were then stained with 25 ng/mL Nile Red (a lipophilic fluorescent dye that stains lipid droplets) (19123, Sigma) in Vectashield containing DAPI for at least 48 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Cell cycle analysis of ploidy by flow cytometry was performed using Hoechst 33342 dye (Sigma Aldrich, St. Louis, MO, USA). HC-derived NSCs were incubated in 10 μg/ml Hoechst 33342 solution for 10 min at 37 °C in culture medium ...
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bioRxiv - Cell Biology 2022Quote: NALM6 target cells were labelled with CellVue membrane dye (CellVue Claret Far Red Fluorescent Cell Linker Kit protocol; Sigma-Aldrich) and 6.25 × 104 or 1.25 × 105 cytotoxic CD8+ T cells were used at indicated effector to target rations ...
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bioRxiv - Neuroscience 2023Quote: ... The uterine horns were exposed by laparotomy and the DNA plasmids (1 μg/μl) with 0.01% Fast Green dye (Sigma Aldrich) were injected in the lateral ventricle using a glass capillary (B100-58-10 ...
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bioRxiv - Pathology 2023Quote: ... mice were injected with 5 µl per gram of body weight of 10 µM Evans Blue dye (E2129, Sigma-Aldrich). Mice were euthanized ...
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bioRxiv - Genomics 2023Quote: ... Excess dye was removed by washing conjugate with sterile DPBS 10 times using 30kDa Amicon filters (cat. UFC503024, EMD Millipore) at 12,000rpm ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Dye prior staining with fluochrome-conjugated anti-CD4 antibodies and DAPI and acquired on an ImageStreamX appartus from Millipore.
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bioRxiv - Molecular Biology 2023Quote: In vivo tumor cell uptake was measured in mice with siDiPRO1 coupled to the fluorescent red dye Cy5 (Sigma-Aldrich) delivered by intratumoral injection at 0.5 mg/kg ...
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bioRxiv - Neuroscience 2023Quote: ... Mice fasted overnight were gavage fed 6% carmine red dye (300 µl) Sigma-Aldrich) in distilled water containing 0.5% methylcellulose (Sigma-Aldrich) and placed in individual cages with white paper covering cage bottoms ...
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bioRxiv - Developmental Biology 2023Quote: 24h AL2-L3 wing discs were incubated for 2.5h at 25°C in Dye medium (see wing disc culture) supplemented with different concentrations of 20E (Sigma, H5142). Discs were then snap-frozen in dry ice ...
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bioRxiv - Microbiology 2023Quote: ... before staining with a 0.1 % (w/v) solution of the DNA-binding dye 4,6-diamidino-2-phenylindole (DAPI) (Sigma, Deisenhofen, Germany) at room temperature for 5 min ...
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bioRxiv - Immunology 2023Quote: ... EVs were further concentrated and unincorporated MemGlow 700 dye was removed by centrifugation with Amicon 15 Ultra RC 10k Da MWCO filters (EDM Millipore). PalmGRET EVs not labeled with MemGlow 700 were also further concentrated after TFF on Amicon 15 Ultra RC 10 kDa MWCO filters ...
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bioRxiv - Microbiology 2023Quote: ... The nylon filter resuspended in 1 mL TSB and plated out onto TSA containing 0.01% Congo red dye (Sigma-Aldrich), Cb60 ...
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bioRxiv - Neuroscience 2023Quote: ... The vascular lumen was labeled by the fluorescent dye rhodamine B-dextran (Wt∼70kD) (Sigma, Cat#R9379, 10 mg/ml) through tail intravenous injection ...
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bioRxiv - Microbiology 2023Quote: ... The slides were washed atleast 4 times with TTBS and before mounting the cells nuclei were visualized by 5 min counterstaining with wash buffer containing Hoechst dye 33342 (1:10,000 dilution, cat no. B2261, Sigma-Aldrich).
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bioRxiv - Biophysics 2023Quote: ... The cell drop was then immersed in 2 mL Leibovitz L15 -/-medium containing 1 μL/mL Hoechst dye (Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was injected into the fourth ventricle at a final concentration of 1-3 µg/µl in addition to trace amounts of fast-green dye (Sigma). Three 50ms square waveform electrical pulses at 5V (E2 ...
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bioRxiv - Neuroscience 2023Quote: ... Reactive species were assessed by treating miBrains with biosensors for nitric oxide (siRNO Nitric Oxide Live Cell Dye, Sigma-Aldrich), peroxynitrite (BioTrack 515 Green ONOO- ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm, Sigma). Coverslips were mounted on glass slides and visualized using CLSM with a 488 nm laser.
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm ...
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bioRxiv - Immunology 2024Quote: ... Infected erythrocytes were labeled with a membrane dye using the PKH26R Red Fluorescent Cell Linker Midi Kit (Sigma #MIDI26-1KT) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was mixed with loading dye and separated on SDS -12% polyacrylamide gel and transferred onto PVDF Membrane (Millipore) before incubation with rabbit anti-OPTN (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... ligand + 1 μl of 6 mM Azide dye] + [100 μl of 10% glucose in 10% glycerol + 4 μl of 20K U/ml stock of catalase (Sigma C3515)] + 8 μl of 500 U/ml glucose oxidase (Sigma G2133)] + 100 μl of 1 M sodium ascorbate.
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bioRxiv - Synthetic Biology 2021Quote: ... Green fluorescence images were used for the detection of sfGFP and red fluorescence images were used for the detection of the sulforhodamine B dye (230162, Sigma-Aldrich), used to detect the correct flow of inputs.
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A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... Fatty acid uptake was measured by treating differentiated cells with 100 μL of Fatty Acid Dye Loading Solution (Sigma-Aldrich, MAK156). Fluorescence intensity (λex= 485nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatants were harvested and cells were lysed with the addition of 300 μl of 2x SDS loading dye (#S3401, Sigma-Aldrich). After freeze thaw cycling the lysates were run on a 10% SDS-PAGE gel ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... or black dye to allow three independent observers to determine identity while watching the tank and in video recordings (Tissue Marking Dye Kit, MDT100, Sigma-Aldrich). Females were also labeled using the same method ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml deionized water, 8 mg of sucrose (A2188.1000, Axon Lab, Switzerland) and 1 mg of Amaranth dye (A1016, Sigma-Aldrich, USA), (ii ...
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bioRxiv - Cell Biology 2019Quote: ... GAGs were precipitated from tissue digests and culture medium (each medium change) using Alcian blue dye (Alcian blue 8GX, 05500, Sigma-Aldrich) saturated in 0.1 M sodium acetate buffer containing 0.3 M MgCl2 (pH 6.2 ...