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Citations for Millipore Sigma :
601 - 650 of 801 citations for Cy7 Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: His-FUS LC was labeled with Atto-488 or Atto-647 NHS-ester fluorescent dyes (ATTO-TEC, Sigma-Alrich). Labeling occurred at or near the N-terminus because only the N-terminus and a lysine at residue position 5 in the leader sequence preceding the N-terminal region hexa-histidine tag were expected to react with the NHS-functionalized dye ...
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bioRxiv - Microbiology 2023Quote: ... 10-fold serial dilutions from 1 x 106 to 1 x 103 cells/mL were incubated with 0.5 mg/mL MTT dye (Sigma) for 2 h ...
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bioRxiv - Physiology 2024Quote: ... C2C12 myoblasts were labelled with a lipophilic dye for 2 minutes at room temperature (cat. no.: PKH67, Sigma, MINI67) as per manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... the cells remaining in the microtube were labeled using the PKH26 lipophilic dye kit (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... 50 µM mutant protein solution was mixed with excess amount of Atto dye in respective binding buffer (Sigma Aldrich) and incubated overnight at 4°C in a dark place ...
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bioRxiv - Cell Biology 2024Quote: ATP levels were determined by staining with 10 μM of the fluorogenic dye BioTracker ATP-Red (Sigma Aldrich, #SCT045). Before measurements ...
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bioRxiv - Cell Biology 2019Quote: Bulk endocytosis and delivery of extracellular materials to the late endosomal compartment was measured using the dye Sulforhodamine 101 (SR101, Sigma), following published protocols (42 ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated with secondary antibody conjugated with 2 µg/ml Alexa dye (Thermo) and 300 nM DAPI (Sigma) in blocking solution for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: This drug susceptibility assay was performed essentially as described [63] and is based on only live cells reducing the blue and non-fluorescent viability indicator dye resazurin sodium salt (‘Alamar blue’; Sigma) to pink ...
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bioRxiv - Biochemistry 2019Quote: ... and absorbance scans were performed on a spectrophotometer (Shimadzu) to identify a region of spectral overlap in absorbance maxima between the proteins and Rose Bengal dye (# 330000, Sigma). The most robust overlap occurred between 550-580 nm (Fig ...
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bioRxiv - Cell Biology 2019Quote: ... Covalently modified αSYN was separated from non-reacted dye on a Sephadex G-25 column (Amersham, GE) and concentrated with 6 kDa MW cut-off spin columns (Millipore). The final concentration of the fluorescently labeled protein was measured by UV-VIS spectrophotometry at 280 nm with E ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells that had invaded the underside of the inserts were fixed with 100% methanol for 10 minutes at −20°C and stained with 0.5% crystal violet dye (EMD Millipore) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The DNA (3–4 μg/μl) together with the dye Fast Green (0.3 mg/ml; Sigma, St Louis, MO, USA) was injected (~1 μl ...
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bioRxiv - Bioengineering 2020Quote: ... cells on different GO and RGO substrates were collected and incubated with BioTracker™ CyP AP dye (10 μM) (Millipore) in MEM medium for 30 min at 37 °C followed by PBS washing.47 Cellular ALP activity catalyzes cleavage of the phosphate group in CyP ...
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bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: The Congo red binding (CRB) ability of the APEC isolates was determined using agar plates supplemented with 0.003% CR dye (Sigma, USA) and 0.15% bile salts ...
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bioRxiv - Microbiology 2020Quote: ... OD600nm adjusted to 0.1 (measured in 100 µL volume in a clear flat bottom 96 well plate) and supplemented with 1.6 mg/ml Red#40 dye (Sigma, Israel). Fifty µL of this culture were pipetted to rows A-G of the plate containing the amoeba ...
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bioRxiv - Molecular Biology 2020Quote: ... All samples were then boiled at 95 °C for 10 mins at a 1:1 (v/v) ratio in 2x Laemmli loading dye (Sigma) before being electrophoresed through a 4-12% NuPAGE Bis-Tris polyacrylamide gel (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: The glycogen concentration was measured by a colorimetric assay based on the peroxidase sensitive dye 3,3′,5,5′-tetramethylbenzidine (TMB) (Sigma-Aldrich #860336). Glycogen was digested to glucose using amyloglucosidase (AG) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein solution was then filtered through a 0.22 μm filter and free dye removed by buffer exchange into gel filtration buffer in Amicon ultra 10 kDa spin concentrator (Millipore). The extent of labeling was estimated based on absorbance at 280 and 647 nm.
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bioRxiv - Molecular Biology 2022Quote: ... On the 6th day post-induction of differentiation the cells were fixed with 3.7% formaldehyde solution followed by staining with Oil Red-O dye (Sigma-Aldrich) (prepared in 60% isopropanol ...
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bioRxiv - Immunology 2022Quote: ... the different mouse groups were killed 1 hour after retro-orbital injection of 100 μl of 2% Evans blue (EB) dye (Sigma) and were perfused intracardially with 15 ml of PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed thrice with 0.1% Triton-X in PBS followed by incubation with secondary antibodies and DAPI (nuclear dye) (Sigma, USA) for 2 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Apoptotic cells were revealed in 2-days-old live embryos by adding the Acridine Orange vital dye (N-4638; Sigma) to the embryo water at a final concentration of 5 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of an overnight culture of OD600 = 5 suspended in water was spotted onto LB without NaCl agar plates containing the dye Congo red (40 μg/ml, Sigma) and Coomassie Brilliant Blue G-250 (20 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Synchronized feeding third instar female larvae were sorted and placed in a hole created at the centre of an agar plate (3.3% wt/vol) filled with yeast paste containing 4% (w/v) FD&C Blue dye # 1 (Sigma Aldrich). The larvae were allowed to feed in the dyed-yeast paste for 2 hours ...
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bioRxiv - Biophysics 2020Quote: ... Unbound dye was removed by repeated dilution and centrifugation through an Amicon® Ultra-15 Centrifugal Filter Unit (Millipore Sigma), prior to size exclusion chromatography ...
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bioRxiv - Developmental Biology 2022Quote: ... Probe sets complementary to cycB (CG3510; 48 oligos) or egfp (32 oligos) were conjugated to Atto 647N dye (Sigma-Aldrich) or Atto 565 dye (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... Pure tubulin and BSA were labeled with commercial NHS-conjugated dyes (Cy5 or Alexa568) according to supplier’s instructions (Sigma: GEPA150101). A similar method was used to conjugate biotin to tubulin (EZ-Link™ NHS-PEG4-Biotin ...
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bioRxiv - Biochemistry 2020Quote: ... Brain slices were mounted on glass slides and incubated for 5 min in the dark with 1μg/mL Hoechst dye (33342 Sigma-Aldrich) in PBS to detect cell nuclei ...
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bioRxiv - Neuroscience 2020Quote: ... was injected into the heart chamber of the fish along with a high molecular weight fluorescent dye (2000 kDa dextran-conjugated FITC (3 mg/ml, Sigma). We estimate that 1 nl of a 10 nM Aβ injection into a ∼3.01 (±0.16 ...
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bioRxiv - Microbiology 2021Quote: ... Labeled lentivirus particles were then purified to eliminate excess free dyes and lipids by ultracentrifugation for one hour at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Microbiology 2019Quote: ... along with the membrane-impermeable nuclear dye Cytotox Green (Essen BioScience Inc. Ann Arbor, MI) and cell-permeable Hoechst stain (Sigma). Cytotoxicity was measured on an ImageXpress Pico (Molecular Devices ...
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bioRxiv - Biophysics 2019Quote: ... Then the sample was rinsed once with deionized water and was incubated with 200 µL 1:106 dye dilution in 0.1 M sodium bicarbonate (792519-500G, Sigma-Aldrich) for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed 3 x 5 min with 3% BSA in PBS-T and incubated with the following DNA dye and secondary antibody dilutions in blocking solution for 3 hours at room temperature: Hoechst 33342 (0.2 mM, Sigma-Aldrich), Goat Anti-Chicken Alexa Fluor® 647 (4 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and the ampicillin media was stained by adding 5 µl and 3 µl of a fluorescent dye (rhodamine, Sigma S1402) in 100ml of media used to grow MG:GT and MG/pBGT cells ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were kept at least 45 min at 33_C in the “recovery” ACSF (similar to cutting ACSF with 2 mM MgSO4 and CaCl2) before incubation with the calcium sensor Cal520AM (AAT biotech) and the glial dye SR101 (Sigma) for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with a fluorometric calcium indicator (Cal520AM, 1:1000, AAT biotech) and glial cell dye (SR101, 1:1000, Sigma) directly in the culture medium for 30min ...
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bioRxiv - Biochemistry 2022Quote: ... and equimolar TCEP for 30 minutes on ice before addition of a 5-fold molar excess of the green fluorescent CF488A maleimide dye (Sigma). The mixture was gently agitated overnight at 4 °C in a 1.5 mL amber tube ...
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bioRxiv - Developmental Biology 2022Quote: ... implantation sites were identified by intravenous injection of 0.1 ml of 1% Chicago blue dye (Sigma-Aldrich, St. Louis, MO) dissolved in saline.
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bioRxiv - Immunology 2022Quote: ... Effector (TILs) and target cells (autologous tumor cell lines) were labeled respectively with 1μM PKH67 and PKH26 fluorescent dyes (Sigma-Aldrich) according to the manufacturer’s protocol and loaded on the chip array ...
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bioRxiv - Synthetic Biology 2022Quote: ... then mixed with 1 mL of the reactive dye 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF) (Sigma Aldrich), previously dissolved in DMSO (20 mg/mL) ...
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bioRxiv - Biochemistry 2022Quote: ... Alexa FluorTM 555 NHS stock solution was prepared immediately before use by solubilizing the dye in DMSO (Sigma-Aldrich, >99.9 %) to stock concentration of 1.6 mM ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Neuroscience 2023Quote: 1×109 particles/ml EMVs and EEXs were pre-incubated with PKH 26 dye (2 μM, Sigma, USA, cat# PKH26GL) [57] ...
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bioRxiv - Physiology 2023Quote: ... Samples were then stained with 25 ng/mL Nile Red (a lipophilic fluorescent dye that stains lipid droplets) (19123, Sigma) in Vectashield containing DAPI for at least 48 hours at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Cell cycle analysis of ploidy by flow cytometry was performed using Hoechst 33342 dye (Sigma Aldrich, St. Louis, MO, USA). HC-derived NSCs were incubated in 10 μg/ml Hoechst 33342 solution for 10 min at 37 °C in culture medium ...
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bioRxiv - Cell Biology 2022Quote: NALM6 target cells were labelled with CellVue membrane dye (CellVue Claret Far Red Fluorescent Cell Linker Kit protocol; Sigma-Aldrich) and 6.25 × 104 or 1.25 × 105 cytotoxic CD8+ T cells were used at indicated effector to target rations ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was mixed with loading dye and separated on SDS -12% polyacrylamide gel and transferred onto PVDF Membrane (Millipore) before incubation with rabbit anti-OPTN (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm ...