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Citations for Millipore Sigma :
601 - 650 of 6791 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... MhOR5 was eluted by adding 2.5 mM desthiobiotin (DTB) and cleaved overnight at 4°C with HRV 3C Protease (EMD Millipore). Sample was concentrated to ∼5 mg/mL and injected onto a Superose 6 Increase column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Cells were separated by centrifugation at 6,000 x g for 5 min and cleaved proteins in the supernatant were concentrated and dialyzed with cleavage buffer using an Amicon filter of 10kD MWCO (Millipore) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded onto HisTrap column and flowthrough containing cleaved protein was pooled and concentrated (Amicon 3 K Sigma Aldrich). Proteins were loaded onto a Superdex 200 increase 10/300 GL column (Cytiva ...
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bioRxiv - Plant Biology 2023Quote: ... The fusion protein was cleaved using Thrombin (from bovine plasma, 50 NIH units/mg dry wt) following the manufacturer’s instructions (Sigma-Aldrich). The following day ...
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bioRxiv - Cell Biology 2020Quote: The antibodies used in this study are as follows: alpha-tubulin (mouse mAb DMIA; T9026; Sigma), Arl13b (mouse mAb N295B/66 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were incubated with purified mAbs (0.1-2.0 µg/mL) diluted in 10% NGS (Sigma-Aldrich) / 0.1% saponin ...
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bioRxiv - Molecular Biology 2022Quote: ... The primary antibodies purchased from commercial sources are listed below: mouse anti-HA mAb (Sigma, USA), mouse anti-FLAG mAb (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... blots were incubated in a 1:10,000 dilution of anti-MBP antibody (EDM Millipore, #MAB 386) in 5% BSA in TBST overnight at 4C ...
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bioRxiv - Biochemistry 2021Quote: ... was initially subjected to affinity chromatography using a protein-A/anti-BSA mAB matrix (Sigma-Aldrich) to remove excess albumin (a heme-binding protein contaminant) ...
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bioRxiv - Neuroscience 2022Quote: ... Type 1 progenitors were identified by immunostaining for GFAP (mouse mAb anti-GFAP; #IF03L, Millipore Sigma) and SOX2 (rabbit polyclonal anti-SOX2 #5603 ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... pH 7.4 for affinity purification of the FLAG-tagged cHMM on M2 mAb-Sepharose beads (Sigma). Bound cHMM was eluted with 0.1 mg⋅mL−1 FLAG peptide (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Antibody binding was detected with goat anti-mouse IgG mAb conjugated with AlexaFluor 647 fluorochrome (Sigma). Sample acquisition was performed on a LSR Fortessa-X20 cytofluorimeter (BD biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse anti-b-actin mAb (clone AC-15; #A1978) was obtained from Sigma-Aldrich (MO, USA). Rabbit anti-GFP pAb was from MBL (Nagoya ...
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bioRxiv - Microbiology 2023Quote: ... HCoV-OC43 rN was additionally characterized by using mouse anti-OC43 N mAb (Sigma-Aldrich # MAB9013) followed by IRDye® 800CW Goat anti-Mouse IgG Secondary Ab (LI-COR # 926-32210).
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used were mouse mAb to MT1-MMP (1:500; clone LEM-2/15.8, Millipore), mAb GAPDH (1:10000 ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... Purified mAbs in PBS were then filtered using sterile 0.2-μm pore size filter devices (Millipore), and stored in aliquots at 4°C until future use.
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bioRxiv - Microbiology 2023Quote: ... incubated with mouse anti-HA mAb clone 12CA5 conjugated with protein G agarose beads (Sigma-Aldrich) for 16 h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The E protein was cleaved from the EGFP-8xHis fusion by incubation of the beads with 150 units of thrombin (Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Biochemistry 2019Quote: ... and the flow-through containing cleaved protein was collected and concentrated to 2 mL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Synthetic Biology 2020Quote: ... The product was cleaved from the support with 15 ml of a 1:1 mixture of 28-30% ammonium hydroxide (Sigma-Aldrich) and 40% aqueous methylamine (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... the TEV cleavage step was done overnight at 4°C and the cleaved proteins were concentrated using 100K MWCO concentrator (EMD Millipore). Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved Lis1 or Ndel1 in the supernatant was collected and concentrated to 500 µl with a 30K MWCO concentrator (EMD Millipore). The concentrated protein was then purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Systems Biology 2023Quote: ... the disulfide bond linking the probe and the fluorophore was cleaved with Cleavage buffer comprising 2×SSC and 50 mM TCEP (Sigma, 646547) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... and the flow-through containing cleaved protein was collected and concentrated to 2 mL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Plant Biology 2020Quote: ... using either 0.5 μg of recombinant histone H3 (Millipore, Cat. #14-411), 2 μg of chicken core histones (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2020Quote: ... 14-3-3 antibody and active recombinant AMPK (α2β1γ1) were from Millipore. Precast Tris-Glycine polyacrylamide gels and 10X tris-glycine gel electrophoresis buffer were from BioRad ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Genetics 2020Quote: ... 0.5 U DNA recombinant Taq polymerase in buffer (pH= 8.0; Sigma, Germany), 1x PCR buffer (pH=8.7 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 ng/mL of recombinant basic fibroblast growth factor (Sigma-Aldrich). RAW 264.7 macrophages were cultured in complete media composed of high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM (1x ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Biochemistry 2021Quote: ... Bovine erythrocyte ubiquitin and recombinant hsNEDD8 were purchased from Sigma-Aldrich (U6253) and BostonBiochem (UL-812 ...
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bioRxiv - Microbiology 2020Quote: ... A set of recombinant RT standards (EMD Millipore, Molsheim, France; catalog# 382129) ranging from 5.12e3 nU/mL to 5.12e9 nU/mL was used to estimate the nU/mL of reverse transcriptase activity in the FIV stock.
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml of recombinant human leukemia inhibitory factor (LIF, EMD Millipore) and 1 mM Valproic acid (Wako) ...
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bioRxiv - Bioengineering 2020Quote: Recombinant human epidermal growth factor (EGF, 5 ng/mL; Sigma-Aldrich, E9644) was used as an invasion promoter and recombinant human nodal (250 ng/mL ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant VEGF (V7259) and TGF-β1 (100-21) was purchased from Sigma and PeproTech ...
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bioRxiv - Immunology 2021Quote: ... were coated with 100 ng/well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... The Red/ET recombinant enzyme was induced by L-arabinose (Sigma-Aldrich) and a temperature shift from 30°C to 37°C ...
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bioRxiv - Physiology 2020Quote: ... then injected with 0.75 IU/kg human recombinant insulin (I2643, Sigma Aldrich) at ZT6 (time “0 minutes”).
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bioRxiv - Biochemistry 2021Quote: Human recombinant SRPK2 (0.4 ug/reaction; EMD Millipore 14-666; Glu46-end) and SRSF2 (0.8 ug/reaction ...
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bioRxiv - Immunology 2021Quote: ... were coated with 100 ng/well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 10 nM recombinant TeNT–light chain (Sigma–Aldrich) for 16 h in a CO2 incubator ...