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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and blocking buffer before overnight incubation at 4 °C with mouse anti-ionized calcium-binding adapter molecule 1 (IBA1; EMD-Millipore cat# MABN92+; 1:150) and rabbit anti-transmembrane protein 119 (TMEM119 ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA with freshly added 1 mM DTT), then concentrated to 500 μl using Amicon Ultra-4 (4 mL, 3 kDa cut-off) (Millipore, Darmstadt, Germany, UFC800324) and loaded onto a Superdex75 10/300 GL size exclusion column (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... nonspecific staining was blocked with blocking solution (TBST and 4% normal donkey or normal goat serum) and incubated overnight at 4°C with SOX9 antibody (1:2000, Millipore, AB5535, RRID: AB_2239761). Sections were washed and incubated with Alexa 568-conjugated secondary antibody (1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: We purchased six-week-old C57BL/6 female mice and used a reported chemical carcinogen 4-nitroquinoline 1-oxide (4-NQO, cat#N8141, Sigma, St Louis, MO) to induce ESCC as previously reported [38] ...
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bioRxiv - Pathology 2023Quote: ... 1-6 months-old adult mice were shaved on the dorsal skin and treated topically with 40 μl of 1-5mg/ml 4-hydroxytamoxifen (4-OHT; Sigma, St. Louis, MO) in ethanol every other day for 4 times ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were transcardially perfused and brains hemisected ∼1 mm past the midline and postfixed overnight in 4% paraformaldehyde (Sigma Aldrich 30525-89-4) at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and methoxy forms of MA with chain lengths of 72-86 carbon atom was obtained from Sigma (M4537, Sigma-Aldrich, St. Louis, MO) [83] ...
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bioRxiv - Bioengineering 2023Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG2000, Laysan Lipids, Arab, AL) were dissolved in propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80°C ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 5% skim milk for 1 hour and incubated at 4 °C overnight with antibodies including α-c-Myc produced in rabbit (Sigma-Aldrich, C3956, 1:2,000), and α-HA high affinity produced in rat (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... BSA (1%) and PBS-T (0.5%) followed by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI) in PBS (1x) (Sigma-Aldrich, St. Louis, MO, 1:1000) for 20 minutes at RT ...
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bioRxiv - Bioengineering 2023Quote: ... the nuclei were stained at room temperature for 15 minuts with 4’,6-diamidino-2-phenylindole (DAPI)(Sigma Aldrich, D9542, 1:500, 1 mg/ml) to yield a blue nucleic fluorescence by binding to nucleic acids.
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bioRxiv - Physiology 2024Quote: ... Samples were eluted directly in a MTP 384 Polished Steel target plate (Bruker Daltonics, Billerica, USA) and diluted (1:1, v/v) with α-cyano-4-hydroxycinnamic acid matrix (Sigma-Aldrich, Saint Louis, USA). Sample-matrix co-crystallization was carried out by the dried-droplet method 20 ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then blocked in 10 % BSA with PBS-Tween 0.1 % for 30 min before incubating overnight at 4 °C with primary antibodies anti-rabbit ARMC2 (1/50) (HPA053696, Sigma Aldrich, Saint-Quentin-Fallavier, France) and anti-guinea pig tubulin (1/100 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
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bioRxiv - Microbiology 2021Quote: ... The blocks were infiltrated with a 4:1 mixture of propylene oxide (PO):Durcupan resin (Sigma) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... at 4°C followed by a contrasting step with 1% thiocarbohydrazide (Sigma-Aldrich, St. Louis, USA) for 1 h at room temperature and 1.5 h incubation with 2% OsO4 ...
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bioRxiv - Cancer Biology 2020Quote: ... All N/Tert-1 cells were also supplemented with 4 μg/ml hygromycin B (Millipore Sigma). All cells not directly purchased from providers were cell type confirmed by Johns Hopkins or MD Anderson cell line authentication services ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10000; D9542, Sigma-Aldrich, St Louis, USA), and mounted on glass slides with Mowiol 4-88 (Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... 1 µL of matrix solution composed of saturated α-cyano-4-hydroxycinnamic acid (Sigma, Lyon, France), 50% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in 1.5% BSA for 1 hr at RT and incubated with 4% PFA (Sigma) for another 24 hr in case of SARS-CoV-2 infected cells ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF) and 1x PhosSTOP phosphatase inhibitor cocktail (Sigma-Aldrich), and centrifuged at 10,000 x g for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclear DNA was stained with DAPI (4’,6-diamidino-2-phenylindole) (1 mg/ml) (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... on 25 × 75 × 1 mm glass slides using Mowoil 4-88 mounting medium (Sigma-Aldrich: 81381). Mounting medium was set at 4°C and slides were imaged within 1 hour.
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bioRxiv - Immunology 2022Quote: ... The slides were then stained with 1/1000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 minutes at room temperature and washed twice for 5 minutes in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with rabbit anti-tyrosine hydroxylase (1:1000 Millipore, USA) in PBS-T and 2% NDS solution ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were incubated for 1 hour at room temperature with 4% BSA (Millipore Sigma, #82-067) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4 °C in guinea pig anti-VGLUT1 (1:1000, Millipore AB5905) or guinea pig anti-VGLUT2 (1:8000 ...
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bioRxiv - Cancer Biology 2021Quote: ... SHP2i was diluted in 50 mM Sodium Citrate Buffer pH = 4 with 1% Hydroxyethylcellulose (Sigma-Aldrich), 0.25% Tween (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were subsequently incubated overnight at 4°C with primary antibodies (S100β 1/200, Sigma S2532 ...
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bioRxiv - Neuroscience 2021Quote: Colonies at selected phases (Tables 1-4) were anaesthetized with MS222 (tricaine methanesulfonate, Sigma, A5040-25) and fixed in 4% PFA in MOPS (0,1 M MOPS ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated at 4 °C overnight with primary antibodies against APP (1:1000, Millipore) or APP-CTFs (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with primary antibodies against GAD-67 (Millipore, 1:2000) and parvalbumin (Swant ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4°C with goat anti-ChAT (1:100, Millipore Sigma-Aldrich, AB144P) and anti-STMN2 (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... The blocks were infiltrated with 4:1 mixture of propylene oxide (PO):Durcupan resin (Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μM Na3VO4) with 1 mM dithiothreitol (DTT) and 4 μg/ml leupeptin (Sigma Aldrich;L2884). Lysates were extracted by further addition of 800 μL of NP-40 buffer ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 µg ml-1 of 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich Chemie GmbH).
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bioRxiv - Cell Biology 2020Quote: ... with 1 μg/mL 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) protease inhibitor (Sigma-Aldrich, Inc.) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2020Quote: ... and reaction overnight at 4 °C with mouse anti-MAP2 (1:2,000; Sigma-Aldrich, #M4403, RRID:AB_477193) and then mouse anti-GAPDH (1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Insoluble aggregates were removed by centrifugation (20,000 x g, 30 min, 4°C; Sigma 1-16K). Solubilized membrane proteins (1 mL ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated overnight at 4 °C with primary antibodies to mouse Foxp4 (Millipore, #ABE74, 1:100), UCP1 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy5-conjugated secondary antibodies (1:200; AP192SA6; Merck Millipore, USA; 17 hrs at 4°C). Similar immunolabeling steps were followed for the subsequent sequential staining ...
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bioRxiv - Cell Biology 2021Quote: ... paraformaldehyde (PFA) and 4-(2-hydroxyethyl)-1-piperazine-ethane-sulfonic acid (HEPES) were purchased from Sigma Chemical (St ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were incubated overnight (4 °C) with mouse anti-NeuN (1:500; Millipore, MAB377), rabbit anti-GFP (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then incubated overnight at 4°C with FLAG antibody (1:500, F1804, Sigma), and incubated with TRITC-conjugated secondary antibody (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were stained with 10.9 μM 4′,6-diamidino-2-phenylindole (DAPI, 1:1000 Sigma). Coverslips were mounted in ProlongGold (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... and probed at 4 ºC overnight with mouse monoclonal anti-FLAG M2 (1:1000, Sigma-Aldrich) and mouse monoclonal anti-α-tubulin B512 (1:5000 ...
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bioRxiv - Developmental Biology 2022Quote: ... 5.0 mM HEPES pH 7.6) diluted in 10,000 MW rhodamine-dextran solution (1:4; Sigma-Aldrich). The mixed solution was injected again into the KV and only embryos with rhodamine-positive KV’s were selected for the experiment ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was counterstained with 1 μg/mL DAPI (4’,6’-diamino-2-fenil-indol; Sigma-Aldrich) and mounted onto glass slides with 20 mM Tris pH8 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich, 1:1000).