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Citations for Millipore Sigma :
6351 - 6400 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Cells were treated with muscle DAMPs (1 μg/ml) or LPS (100 ng/ml for 24 hours and Dex (Sigma-Aldrich, D4902, 1×10−8 M) was further directly added in the well for 6 hours ...
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bioRxiv - Cell Biology 2020Quote: ... filled with STORM buffer (25 mM β-mercaptoethylamine [Sigma; 30070], 0.5 mg/ml glucose oxidase [Sigma; G2133], 67 μg/ml catalase [Sigma; C40], 10% dextrose [Sigma; D9434], in 100 mM Tris, pH 8.0) and covered with an empty coverslip ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 U/ml penicillin and 0.1 mg/ml streptomycin (EmbryoMax ES Cell Qualified Penicillin-Streptomycin Solution 100X; EMD Millipore, TMS-AB2-C or Gibco, 15140–122) at 37°C in 5% CO2-buffered incubators ...
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bioRxiv - Biochemistry 2019Quote: ... after which the cells were harvested by centrifugation at 5000 rpm and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were then fixed for 30 min at RT in fixing solution (40% [v/v] methanol, 2% [v/v] acetic acid) and stained overnight with Brilliant Blue G-Colloidal Concentrate (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... were homogenized with 1 ml H20 and extracted by vortexing with 2 ml of chloroform/methanol 2:1 (v/v) and 10 μl of synthetic internal standard (DAG 15:0/15:0, Sigma Aldrich), sonicated for 30 s ...
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bioRxiv - Biochemistry 2020Quote: ... after which the cells were harvested by centrifugation at 5000 rpm and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
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bioRxiv - Microbiology 2021Quote: Metabolites were extracted from cell pellets through addition of 50 μl chloroform and vigorous vortexing, followed by 200 μl methanol/ultrapure water (3:1, containing 5 μM scyllo-inositol (Sigma I8132) as internal standard ...
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bioRxiv - Molecular Biology 2020Quote: ... at 30 volts for 1 hour using 1x transfer buffer (25 mM Tris base, 192 mM Glycine [pH 8.3]) with 10 % methanol (Sigma-Aldrich, #322415). Membranes were blocked for 1 hour with 5 % skim milk in TBS-T (20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...
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bioRxiv - Bioengineering 2019Quote: The freeze-substitution media consisted of 0.2% (w/v) uranyl acetate (SERVA Electrophoresis GmbH, Heidelberg, Germany) in absolute acetone (diluted from 20% uranyl acetate stock in methanol (Sigma-Aldrich)) ...
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bioRxiv - Biophysics 2019Quote: ... Of each lipid 10µl at 3mM dissolved in chloroform/methanol (3:1) were deposited in a glass plate coated with indium tin oxide with about 100Ω resistivity (Sigma-Aldrich) and placed 60 minutes in the desiccator for complete solvent evaporation ...
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bioRxiv - Biochemistry 2020Quote: ... after which the cells were harvested by centrifugation and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
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bioRxiv - Microbiology 2020Quote: ... The residue was then resuspended in 100 µl methanol and one µl of each sample analysed by thin layer chromatography (TLC) using silica gel 60 F254 (Merck Millipore, Germany) with chloroform-acetic acid-ethanol (90:5:10 [vol/vol] ...
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bioRxiv - Physiology 2021Quote: ... Samples were then immersed in 5% (v/v) H2O2 in methanol for up to 3 hours (Sigma-Aldrich Cat No: H1009) for bleaching ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed twice with PBS and extracted with mass spectrometry grade 80% methanol (Fisher, A456-1) and 20% water (Fisher, W6500) supplemented with 1 nmol DL-Norvaline (Sigma, N7502). Flash frozen tumors/tissues were pulverized with liquid nitrogen in a mortar and pestle on dry ice ...
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bioRxiv - Cancer Biology 2022Quote: ... 400 µg protein equivalents of tissue were extracted with mass spectrometry grade 80% methanol (Fisher, A456-1) and 20% water (Fisher, W6500) supplemented with 1 nmol DL-Norvaline (Sigma, N7502). Tissue or Insoluble material from cell extracts were used to calculate protein equivalents by resuspension in 0.2 M NaOH ...
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bioRxiv - Biochemistry 2022Quote: ... Protein pellet of each SA+ and SA-group was obtained by removing the aqueous and organic layers consecutively following the addition of 4 volumes of methanol (Sigma, #34860), 1 volume of chloroform (ACROS Organics ...
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bioRxiv - Biochemistry 2022Quote: ... for 24 hours and serum starved with OptiMEM for 48 hours prior to fixation with 3% PFA/PBS for 3 minutes in room temperature followed by a 5 min incubation at −20 °C with pre-cooled methanol (Sigma Aldrich). Fixed cells were blocked with 3% BSA (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The PBS buffer was then replaced with DMEM with 10% FCS and the cells were further incubated for 3 h at 37°C before being fixed with methanol and acetone (1:1 in volume) mixture and stained with 20% Giemsa stain (Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: We extracted glucosinolates by passing the 70% methanol leachates through columns of DEAE-Sephadex A-25 (Sigma Aldrich, St. Louis, USA) that had been equilibrated in 20 mM sodium acetate for one hour ...
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bioRxiv - Plant Biology 2024Quote: ... polymorpha plant material was extracted with 1 mL of cold methanol containing 5 mM of NaCl to quench enzymatic activities72 and 900 ng of dodecane as internal standard (#297879, Sigma Aldrich). Mechanical disruption was achieved using a TissueLyser II (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were dehydrated in series of Methanol and cleared using BABB (a mixture of 1 part benzyl alcohol to 2 parts benzyl benzoate; Sigma-Aldrich, benzyl alcohol ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were fixed with ice-cold acetone/methanol (1:1 v/v) and a specific antibody binding was blocked with 5% BSA (w/v, Sigma-Aldrich). Organoids were then incubated with primary antibodies against acetylated α-tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2022Quote: ... The evaporated extract was reconstituted in methanol and separated by thin layer chromatography using TLC-silica gel 60 f254 (Sigma-Aldrich) with solvent ethyl acetate ...
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bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
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bioRxiv - Plant Biology 2023Quote: ... Free-hand root cross-sections obtained with sharp razor blades were placed into distilled water and subsequently stained with fresh fluorol yellow 088 (0.01% w/v, in methanol) (Sigma-Aldrich, Merck) at room temperature for 1 h in darkness and rinsed and kept into distilled water before observation ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained with DMEM+10%FCS for 3 hours and fixed with acetone/methanol (1:1 ratio) and stained with Giemsa stain (Sigma-Aldrich). Stained cell images were taken by EVOS XL imaging system (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... After the last EtOH wash the coverslips were incubated with methanol containing 5% acetic acid and 1% (3-Aminopropyl)triethoxysilane (Millipore-Sigma) overnight in a vacuum desiccation chamber in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were equilibrated in pre-chilled transfer buffer with 15% methanol and then protein was transferred to a 0.45 µm nitrocellulose membrane (Sigma catalog# 10600002) via wet electroblotting on ice for 1 h at 300 mV ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue sections on Visium Spatial Gene Expression Slide were fixed with methanol at −20 °C for 30 min and labelled with an anti-αSMA antibody (Cy3 conjugated, 1:2000, Sigma C6198) for 30 min at room temperature followed by DAPI nuclear counter stain ...
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bioRxiv - Microbiology 2019Quote: Sediments from a depth of 28 cmbsf were incubated in triplicate for 10 days in the dark with either 2 mM 99% 13C-labeled or unlabeled (control) sodium bicarbonate (NaHCO3; Sigma-Aldrich, St. Louis, MO, USA) at 10 °C ...
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bioRxiv - Cell Biology 2021Quote: Perfluorononanoic acid (PFNA, 97%), 3-Isobutyl-1-methylxanthine (IBMX, ≥ 99%) and 2’,7’-dichlorofluorescin diacetate (DCFH-DA, ≥ 97%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). JC-1 Mitochondrial Membrane Potential Detection Kit and MitoView™ Green were purchased from Biotium (Hayward ...
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bioRxiv - Plant Biology 2023Quote: ... The foliar hormone sprayed plants and control plants were moved into a transparent Plexiglass chamber for stable isotope labeling using 13CO2 (99+ atom%, Sigma Aldrich, St. Louis, Missouri, USA). This chamber was incubated within a Conviron walk-in plant growth chamber (model no ...
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bioRxiv - Biophysics 2021Quote: ... The Peak fraction was collected and concentrated to 2.5-3.5 mg ml−1 in 100 kDa MWCO Amicon concentrator (Millipore). The A260/A280 value of the protein is lowered to 0.58-0.62 after size-exclusion chromatography.
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bioRxiv - Neuroscience 2021Quote: ... We plated 1 μL of the transformation onto an LB + carbenicillin (100 μg/mL, Sigma-Aldrich, #C9231-1G) agar plate to confirm transformation efficiency and the rest of recovery suspension was placed into 0.5 L LB + carbenicillin (100 μg/mL ...
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bioRxiv - Genetics 2021Quote: ... 2 mM EDTA and 1% Triton X-100) with 20 mg/mL lysozyme from chicken egg (Sigma, L4919) using a tissue grinder (BMS BC-G10 ...
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bioRxiv - Biophysics 2020Quote: ... The cell pellets were suspended in 5 ml pure water at 4 ℃ with 0.4 % TritonX-100 (Sigma-Aldrich), 2 μl DNAase Ⅰ(Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos with dissociated blastomeres enclosed in the fertilization membrane were incubated with 100 µg/ml cycloheximide (Sigma, C7698) in CMFM ...
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bioRxiv - Genomics 2021Quote: ... Samples were homogenized by adding one half of the TRI-Reagent (Sigma-Aldrich, 1 ml per 100 mg) to each sample following sample disruption by using the Precellys 24 Tissue Homogenizer (Bertin ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were stained with fresh smFISH Wash Buffer A supplemented with 100 ng/mL DAPI (Sigma, #D9542-1mg) and incubated in the dark at 37°C for 30 min ...
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bioRxiv - Genetics 2019Quote: ... 1 ml of supernatant was added per 100 μl of Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich) and incubated overnight at 4°C in a tube rotisserie rotator (Barnstead international) ...
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bioRxiv - Neuroscience 2019Quote: ... Striatal or midbrain structures were dissected in 2 mL of PBS-glucose and cells were mechanically dissociated and plated in previously coated in Poly-L-ornithine (1/100) and 2.5μg/ml laminin (Sigma) 96 wells plates for LDH assay and 24 well plates with glass coverslips for immunocytochemistry ...
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bioRxiv - Cell Biology 2019Quote: ... medium was replaced with serum-free DMEM containing PDGF-BB (2-50ng/ml) and 100 μM hydroxyurea (Sigma). Wells were imaged at t0 ...
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bioRxiv - Genetics 2020Quote: ... in PBS containing 0.1% Triton X-100 with 0.5% DMSO and Escin (0.05 mg/mL, Sigma-Aldrich, E1378) at room temperature with gentle agitation for 2 days ...
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bioRxiv - Cell Biology 2020Quote: ... To arrest the ribosome on translated mRNAs cells were treated with 100 µg/mL cycloheximide (CHX) (Sigma-Aldrich) for 10 min at 37 °C and 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... SEC was performed using a Sepharose CL-2B 10/300 column (Sigma-Aldrich, cat. no. CL2B300, 100 mL) injected with lysates from HEK293T/17 or A673 cells ...
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bioRxiv - Neuroscience 2021Quote: ... A single colony was selected for growth in terrific broth (Fisher) supplemented with 100 μg/mL ampicillin (Sigma). Growth was monitored to log-phase protein production induced by isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... The tissue was then moved to warm 5ml 10mM EDTA-PBS containing Liberase TM (100 μg/mL, Sigma) and DNaseI (2 U/mL ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...