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Citations for Millipore Sigma :
6351 - 6400 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Each strain to be tested was then washed twice with sterile 1x PBS and adjusted to a concentration of ~2×105 cells/mL in macrophage growth media containing 1 μg/mL of the anti-GXM antibody 18B7 (Sigma Cat# MABF2069). Cells were opsonized in this media for 1h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 500 to 1000 μl L-15 full medium were added to the suspension and the solution was seeded on 1 - 2 peanut lectin (Sigma-Aldrich, USA) coated glass bottom petri dishes (ibidi ...
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bioRxiv - Neuroscience 2024Quote: ... were trained on a 2.5% agar plate (100mm petri dish) covered with 2 mL of 1 M sucrose solution (SUC, Sigma, Cat. No. S1888) or 0.1% quinine hemisulfate solution (QUI ...
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bioRxiv - Microbiology 2024Quote: ... Each strain was grown in liquid mineral medium (LMM) [43] supplemented with 2 g.L -1 of 99% 13C6-glucose (Sigma-Aldrich, Munich, Germany) as a sole carbon source ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 minutes before incubating in c-Fos primary antibody for 2 days (rabbit anti c-Fos, 1:1000; Millipore, Burlington, MA). Sections were then washed in 3x in TBS with 0.3% Triton X-100 (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 137 mM NaCl2) before blocking for 1 hour at RT with blocking solution (2% BSA IgG free and protease free (Sigma-Aldrich, A3059); 0.1 % Tween 20 (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... cells were permeabilized in 0.5% v/v Triton X-100 for 5 min in 1x DPBS and blocked with 1% BSA in IF wash buffer (1% w/v BSA, 2% v/v Triton X-100; 0.41% v/v Tween-20, EMD Millipore, 1x DPBS) for 1 hr ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were synchronized by bleaching and Day 1 adults were plated on NGM plates containing 100 µM 5-Fluoro-2′-deoxyuridine (FuDR) (Sigma Cat. F0503) with or without 4 mM methyl viologen dichloride hydrate (paraquat ...
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bioRxiv - Bioengineering 2024Quote: ... cells were permeabilized in 0.5% v/v Triton X-100 for 5 min in 1x DPBS and blocked with 1% BSA in IF wash buffer (1% w/v BSA, 2% v/v Triton X-100; 0.41% v/v Tween-20, EMD Millipore, 1x DPBS) for 1 hr.
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bioRxiv - Biophysics 2024Quote: ... Cells were mixed with fluorescent microparticles in a ratio of approximately 1000:1 and resuspended in 2 % polyvinylpyrroli-done (Sigma-Aldrich, P5288) to reach a cell concentration of 25000 cells per μL shortly before cells were injected into collagen ...
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bioRxiv - Cell Biology 2024Quote: ... lentiviral particle production and viral transduction LV-ShOpa1 clone #1 (TRCN0000091111) and LV-SHOpa1 clone #2 (TRCN0000348537) (Sigma, St. Louis, MO, USA) and pLKO.1 ShCtrl and psPAX2 (#12260 ...
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bioRxiv - Cell Biology 2024Quote: ... Staining Solution was prepared by freshly diluting 2 μL of LTR stock solution (1 mM LysoTracker Red DND-99; Sigma Aldrich, L7528) in 1 mL of medium ...
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bioRxiv - Neuroscience 2023Quote: ... OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα) (1:4000) (Supplementary Table 2) and then seeded on coverslips coated with poly-D-lysine (20 μg/ml) (Sigma-Aldrich; P0899) and incubated at 37°C and 10% CO2 in Sato medium ...
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bioRxiv - Molecular Biology 2023Quote: Total fatty acids were extracted from the liver using a 1:2 (v/v) mixture of chloroform/methanol in the presence of 10 nmol of tridecanoic acid (Sigma-Aldrich #T0502) and 10 nmol of phosphatidylcholine (PC ...
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bioRxiv - Neuroscience 2023Quote: ... 1:125 in blocking buffer for 2 hours and tertiary antibody solution (streptavidin-horseradish peroxidase conjugated, 1:250 in blocking buffer, Sigma-Aldrich, RPN1231V) for 2 hours before being exposed to DAB (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Republic of Korea) and cultured in micro-drops of KSOM medium with 1/2 amino acids (EmbryoMax®; EMD Millipore, Billerica, MA) in a humidified atmosphere of 5% CO2 at 37°C until use.
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 ml of RPMI + 2% Bovine Serum Albumin (BSA, heat shock fraction, protease free, essentially globulin free, pH 7, Sigma, Catalog #A3059) to a concentration of ∼2 x 108 CFU/ml ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 hours at room temperature followed by administration of primary antibodies such as ChAT (goat polyclonal, 1:500, EMD Millipore AB144P), Pax2 (rabbit polyclonal ...
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bioRxiv - Pathology 2023Quote: ... the samples were rinsed (1 × 10 sec) and washed with PBS (2 × 5 min) before mounting with glycerol (G5516, Sigma Life Science) and covering with #1.5 coverslips of 22 mm × 22 mm (631-0125 ...
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bioRxiv - Cell Biology 2023Quote: ... in 30 ml Dulbecco’s minimal essential medium (DMEM) containing 1 % (v/v) penicillin/streptomycin and 2% FBS (F7524, Batch 024M3398, Sigma-Aldrich, Missouri, USA), at 37°C overnight in a rotary shaker ...
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bioRxiv - Plant Biology 2023Quote: ... 100 ng purified recombinant CTR1-KD or 1 µg MPK4 was mixed with 2 µg Myelin Basic Protein (MBP, a common kinase substrate, Sigma Cat. M1891) and 1 mM ATPγS in kinase reaction buffer (10 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Hepatocytes were permeabilized for 1 h at room temperature in blocking buffer consisting of 2% Bovine Serum Albumin (Millipore Sigma cat A2153) and 0.2% Triton X-100 (Millipore Sigma cat 648466 ...
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bioRxiv - Biophysics 2022Quote: ... After lipid extraction against organic solvent — CHCl3/MeOH 2:1 (vol/vol) — samples were spotted on silica plates (Sigma-Aldrich, Steinheim, Germany) with the automatic TLC sampler 4 (CAMAG ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301 ...
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bioRxiv - Physiology 2023Quote: ... glycogen phosphorylase was inhibited by IV (jugular vein) injection of 1-(3-(3-(2-Chloro-4,5-difluorobenzoyl)ureido)-4-methoxyphenyl)-3-methylurea (Sigma, 5 mg/kg) one hour prior to the start of a tracer infusion ...
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bioRxiv - Neuroscience 2023Quote: ... resuspended in staining buffer (Tween 0.02%, and 2% BSA in the tris base buffer) and stained with anti-NEUN (1:300, Sigma #FCMAB317PE or #MAB377A5) and anti-CHX10 (1:600 ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were incubated with 50μL primary antibody solution (dilution 1:1000) in 2% BSA (Bovine Serum Albumin, Sigma Cat. No.: A7906) at 4°C cold room overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: Zoospores were adhered for 5 min to plasma cleaned 96-well plates (MatriPlate, Cat. No. MGB096-1-2-LG-L) coated with 0.5 mg/mL Concanavalin A (Sigma, Cat. No. C2010). We fixed the cells in 4% PFA and 50 mM sodium cacodylate ...
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bioRxiv - Genetics 2023Quote: ... The resulting F2 embryos were collected and raised in embryo media (E3) that contained 200 μM 1-phenyl 2-thiourea (PTU; Sigma Aldrich #P7629) to inhibit pigmentation28 ...
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bioRxiv - Neuroscience 2024Quote: ... The tip of the pipette was backfilled with ∼1-2 µl calcium ionophore I cocktail A (Sigma-Aldrich, St. Louis, Missouri, USA). The rest of the electrode was filled with ∼15 µl CaCl2 (2 M) ...
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bioRxiv - Microbiology 2024Quote: ... S6734) and Etravirine (Catalog No. S3080) were from Selleckchem (Texas, USA). AKTi-1/2 (Catalog No. 124018) was from Sigma (Missouri, USA). DharmaFECT transfection reagent (Catalog No ...
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bioRxiv - Cancer Biology 2024Quote: ... Negative controls consisted of two sections from each group that were incubated for 2 hours at 37 C with 1 mg/ml hyaluronidase (Sigma Cat# H3884) in 20 mM sodium phosphate buffer ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was isolated from Nb-1 shoots with the Spectrum Plant Total RNA kit (Sigma-Aldrich) with on-column DNase I digestion (New England Biolabs) ...
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bioRxiv - Genetics 2020Quote: ... and diluted to measure protein concentration (1:10 diluted samples, Bradford Assay Kit, Sigma-Aldrich, B6916) and ATP level (1:2,000 diluted samples ...
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bioRxiv - Microbiology 2022Quote: Extraction method 1 used a modified protocol with the GenElute Bacterial genomic DNA kit (Sigma-Aldrich). The pellet was resuspended in 180 μl of kit-included lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: The transporter activity of MDR-1 was assessed using the MDR1 Efflux Assay Kit (ECM910, Millipore), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Pop 9-12) (Figure 1) was isolated using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich). DNA-purity and - quantity was assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Genetics 2024Quote: ... Cell lysis took place in 1 ml of EZ-ChIP kit lysis buffer (Millipore, 17-371RF), and chromatin was sheared into approximately 1000 bp fragments via sonication using a Diagenode Pico Bioruptor ...
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bioRxiv - Biochemistry 2021Quote: ... lung or intestinal tissue were fluorescently labeled via reductive amination with 2-aminobenzamide (2-AB) (Sigma-Aldrich Canada)42 ...
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bioRxiv - Bioengineering 2022Quote: ... and antimycin A (AA) (500 nM) and 2-deoxy-D-glucose (2-DG) (25 mM, all Sigma-Aldrich) were prepared in XF assay medium and loaded into the appropriate injection ports on the cartridge plate and incubated for ten minutes in a non-CO2 incubator at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... coli in 2% alginate solution supplemented with 2% Luria-Bertani (LB) broth (Sigma Aldrich, St. Louis, MO, USA). For ink preparation ...
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bioRxiv - Microbiology 2019Quote: ... 2 % [w/v] glucose) with or without 2 mM of t-BOOH (Luperox® TBH70X, Sigma-Aldrich, USA) and approx ...
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bioRxiv - Microbiology 2019Quote: ... using either 2-Deoxy-D-galactose (DOG) at a final concentration of 2 mg/mL (Sigma-Aldrich, D4407) or Azidothymidine (AZT ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were suspended in ice cold HBSS (2% serum) containing 2 μg/ml Propidium iodide (Sigma-aldrich,USA) for dead cell discrimination ...