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Citations for Millipore Sigma :
6351 - 6400 of 7005 citations for Caspr Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Embryos were then washed 3 times for 20 min in PBS-Triton before incubation with anti-rabbit Alexa Fluor 546 used at 1:500 dilution in blocking buffer containing Hoechst 33342 (Sigma) to label the nuclei ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections of 50 nm were blocked with 5% fetal bovine serum/5% normal goat serum for 30 min and subsequently incubated with rabbit anti-HA antibody (Sigma) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: The primary antibodies used were: a rabbit polyclonal anti-GFP in a 1:400 dilution (Millipore Sigma, AB3080, lot: 3717722), a mouse monoclonal anti-NeuN in a 1:500 dilution that is a marker for mature neurons (Millipore Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... as primary antibodies and Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed with PBS-0.1% Tween 20 and then incubated with 100 μl of 100 μg/well of biotinylated rabbit anti-human C1q (Sigma) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The wells were washed as before and then probed with 100µl rabbit anti-chicken IgG conjugated to horseradish peroxidase (HRP) (Sigma Ltd.) diluted 1:3000 in ELISA diluent for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The antibodies used in this study were: polyclonal rabbit anti-human HINT1 antibody (1:1000, Sigma, San Luis, MO, USA), and to demonstrate equal loading mouse monoclonal anti-β-actin antibody (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... The precleared supernatant was divided into two Eppendorf tubes and beads were either cross-linked to the bait antibody (Grin2b-rabbit #06-600 Millipore,Ppp1ca-Abcam Rabbit #ab16446 ...
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bioRxiv - Cancer Biology 2023Quote: ... PLA reactions were subsequently carried out using Duolink PLA plus and minus probes for rabbit and mouse (respectively) and Duolink In Situ Detection Reagents Orange (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The protein bands were obtained using horseradish peroxidase-conjugated antibodies against mouse whole immunoglobulins and horseradish peroxidase-conjugated antibodies against rabbit whole immunoglobulins (1∶10,000, Sigma-Aldrich) as secondary antibodies and detection with the Lumi-Light Plus Western Blotting Substrate (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903, Sigma) at 1:5,000 dilution at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore; sheep anti-TH ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in primary antibody (Rabbit anti-GFP 1:1000 in blocking buffer, Cat# AB3080, Millipore Sigma, St. Louis, MO) overnight at room temperature on an orbital shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002 ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies coupled to horse radish peroxidase (goat anti-rabbit, goat anti-rat, and goat anti-mouse) were purchased from Sigma and used at the recommended dilution.
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bioRxiv - Neuroscience 2024Quote: ... This was followed by incubation for 90min at room temperature (RT) with secondary antibody anti-goat or anti-rabbit IgG Biotin (Sigma) diluted 1:400 or 1:300 ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were labeled with a rabbit anti-KIF5B antibody (Tanaka et al., 1998) and a mouse anti-insulin antibody (Sigma), followed by Alexa-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated with 1:5 diluted Duolink® in Situ PLA Probe anti-rabbit Plus (cat#DUO92002, Sigma-Aldrich) and anti-mouse Minus (cat#DUO92004 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were transduced with a rabbit Cav1.2-EGFP expression vector (Navedo et al., 2010) in the presence of 1 μM brefeldin A (Sigma Aldrich) at 19.5°C overnight ...
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bioRxiv - Immunology 2024Quote: ... The infectious bloodmeal consisted of a 2:1 mix of washed rabbit erythrocytes (BCL) supplemented with 10 mM adenosine triphosphate (Sigma) and viral suspension supplemented with Leibovitz’s L-15 medium (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... was used in combination with a secondary anti-rabbit antibody conjugated to horseradish peroxidase (Sigma-Aldrich A8275 1:10 000). For visualization ...
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bioRxiv - Immunology 2024Quote: ... Hyde) were cultured for 4 days at 37ºC in BSK-II media and complete with 6% rabbit serum (Millipore Sigma) at pH of 7.6 ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with rabbit complement sera (1:3 dilution, Cat. no. S7764, Sigma) for 30 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with RPMI 1640 and then incubated with 20 μg/ml rabbit anti-hamster IgG (H+L) (Sigma-Aldrich) in RPMI 1640 for the indicated times at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with RPMI 1640 and then incubated with 20 μg/ml rabbit anti-hamster IgG (H+L) (Sigma-Aldrich) in RPMI 1640 for the indicated times at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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bioRxiv - Cell Biology 2020Quote: Cells were stained with rabbit anti-GlActin antibody 28PB+1 (Paredez et al., 2011) and mouse monoclonal anti-HA (Clone HA7, Sigma-Aldrich) both diluted 1:125 in PMEBALG and incubated over-night ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with Alexa-488 goat anti-mouse and Alexa-555 goat anti-α-rabbit (Sigma-Aldrich, St. Louis, MO) (diluted 1:150 in PMEBALG ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained using the blocking solution containing rabbit polyclonal anti-TRPV2 antibody (1:200, ACC-039, Alomone labs) or rabbit polyclonal anti-FLAG antibody (1:100, F7425, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with Alexa 488/647-conjugated goat anti-mouse or anti-rabbit (1:100-1:400, Thermo Fischer) and Alexa 488/546-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were blocked for at least 2.5 hours at room temperature followed by the addition of poly-clonal rabbit anti-histone H4 (citrulline 3) antibody (EMD Millipore) used at 1:100 and incubated overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... the blot was washed in PBST and incubated in primary antibodies of rabbit polyclonal anti-TBX5 at a 1:400 dilution (Sigma #HPA008786) and mouse monoclonal anti-cTNT at 1:1000 dilution (ThermoFisher Scientific #MS-295-P) ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were then washed and probed with either goat anti-mouse horseradish peroxidase or goat anti-rabbit horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10000 for 1 hour (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for immunoprecipitation: HSF1 (ADI-SPA-901, Enzo), HSF2 (Östling et al., 2007) and normal rabbit IgG (EMD Millipore). Crosslinks were reversed by incubating the samples at 65°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then labeled with the PLA antimouse PLUS probe (DUO92001) and the PLA anti-rabbit MINUS probe (DUO92005) purchased from Sigma-Aldrich. Detection was performed with Duolink in situ detection reagents red kit (DUO92008 ...
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bioRxiv - Developmental Biology 2021Quote: ... CST, 4967), Alpha-Tubulin (1/3000; CST, 2144) at +4°C followed by incubating with HRP conjugated anti-rabbit (Sigma-Aldrich) or anti-mouse (CST ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated at 37ºC for 1 h with Duolink® in situ PLA® Anti-rabbit PLUS and Anti-mouse MINUS (Sigma) and later washed with 0,01% PBS-tween ...
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bioRxiv - Developmental Biology 2020Quote: ... slices were washed three times in PBS for 5min and incubated with biotinylated anti-rabbit IgG (1:500 in PBS, Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Genomics 2022Quote: ... and 800ul of antibody buffer containing 1ul of primary antibodies (normal rabbit IgG, Santa Cruz Cat no sc-2027, H3K27me3 (Millipore, Catalogue number 07-449 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibodies goat anti-Olig2 (1:100, R&D AF2418) and rabbit anti-NeuN (1:100, Millipore ABN78) for two days at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... We use a combination of antibodies (rabbit anti-parvalbumin, 1:5000, PV235, Swant + biotin-conjugated lectin from WFA, 1:250, L-1516, Sigma-Aldrich). On the day after ...
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bioRxiv - Molecular Biology 2020Quote: ... the cryosections were incubated with Alexa FluorTM 488 goat-anti-Rabbit IgG (1:1000) for 2 hours at room temperature and then the nuclei counterstained with Hoechst 33342 (Sigma-Aldrich). The cryosections were imaged using the A1+ Confocal Microscope System with NIS-Elements Advanced Research software (Nikon ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4 °C in vehicle with primary antibodies (mouse anti-Syt7, 1:200, Neuromab N275/14; rabbit anti-TH, 1:500, Millipore AB152). After 3 washes for 30 minutes in vehicle ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were probed with anti-gePABP (1:2000) primary antibodies (77) and horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibodies (Sigma-Aldrich) (1:50000) ...
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bioRxiv - Microbiology 2020Quote: ... measuring the decrease in absorbance at 340 nm 30 min after addition of 5 U of L-lactic dehydrogenase (from rabbit muscle, Sigma-Aldrich) and 4 U of pyruvate kinase (from rabbit muscle ...
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bioRxiv - Molecular Biology 2021Quote: ... PLA was performed according to the manufacturer’s instructions using the following kits and reagents: Duolink in-situ PLA Probe Anti-Rabbit PLUS (Sigma-Aldrich # DUO92002), Duolink in-situ PLA Probe Anti-Mouse MINUS (Sigma-Aldrich # DUO92004) ...
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bioRxiv - Physiology 2020Quote: ... Proteins were detected after incubation of the membranes with secondary GP anti-rabbit alkaline phosphatase-conjugated antibody (1:30000, cat# A3687, Sigma Aldrich), GP anti-mouse alkaline phosphatase-conjugated antibody (1:20000 ...
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bioRxiv - Cell Biology 2019Quote: ... anti-GFP (in house made rabbit polyclonal antibody; 1:1,000-4,000) anti-Vinculin (mouse monoclonal; clone hVIN-1; Sigma-Aldrich; 1:10000), anti PMF1/NNF1 (in house made mouse affinity purified monoclonal ...