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Citations for Millipore Sigma :
6251 - 6300 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the resulting powders were employed for total RNA extraction with “Spectrum Plant Total RNA” kit (Sigma-Aldrich, St. Louis, MO, USA) according to manufacturer’s protocol (20).
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bioRxiv - Microbiology 2023Quote: ... the reactions were heated to 90 °C for 10 minutes and then allowed to gradually cool to room temperature and RNA was purified using the GenElute Mammalian Total RNA kit (Sigma RTN350) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from plant tissue samples using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St Louis, MO, USA) followed by DNase digestion with RNase free DNase Set (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was immediately extracted from pooled samples after grinding using a Spectrum™ Plant Total RNA Kit from Sigma-Aldrich (STRN250) and an On-Column DNase I Digestion Set from Sigma-Aldrich (DNASE70-1SET ...
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bioRxiv - Developmental Biology 2024Quote: ... and pCMV-Sport6.ccdb-marcksl1.L were linearized with EcoRV followed by synthesis of anti-sense RNA probes using the digoxigenin RNA Labeling Kit (Sigma Aldrich) and SP6 polymerase ...
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bioRxiv - Cell Biology 2020Quote: ... complete medium containing 20 mM 2-deoxyglucose (2-DG; Carl Roth GmbH, Karlsruhe, Germany) and 10 M antimycin-A (Sigma-Aldrich, Steinheim, Germany) for at least 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... The FahPM/PM mice were kept on water supplemented with 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. #: PHR1731-1G). Mice with more than 20% weight loss were humanely euthanized according to IACUC guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... 2-(5-Benzo [1,3] dioxol-5-yl-2-tert-butyl-3HImidazol-4-yl)-6-methylpyridine hydrochloride hydrate (SB505124) (Sigma-Aldrich; St. Louis, MO) and implanted subdermally ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Cell Biology 2021Quote: ... the affinity gel was suspended in elution buffer (2% SDS, 50 mM Tris pH 7.5, 50 mM NaCl, 20% glycerol, 2 mM dithiothreitol (Sigma-Aldrich #D0632-5G; fresh) and incubated at 65°C for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Strains were routinely grown under yeast conditions (media at 30°C) in YPG (1% yeast extract, 2% Bacto peptone, 2% glucose) or under filament-inducing conditions using RPMI medium (Sigma, St. Louis, MO) with MOPS (morpholinepropanesulfonic acid ...
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bioRxiv - Plant Biology 2021Quote: ... total soluble protein was extracted from 50 mg of ground frozen shoot material in 250 µl 2× Laemmli buffer supplemented with 1% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich, Steinheim, Germany). Proteins were denatured for 10 min at 90°C and separated using 10% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: The mouse was induced by injecting 2 month-old female BALB/c mice with2 mg human-derived proteoglycan (PG) together with 2 mg DDA (Sigma, MO, united States). as described previously (1) ...
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bioRxiv - Zoology 2020Quote: ... 3-methyl-but-2-enyl acetate and (2E)-hex-2-enyl acetate were confirmed with analytical standards from Sigma-Aldrich (St. Louis, MO). For the analysis of CHCs ...
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bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300 ...
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bioRxiv - Microbiology 2022Quote: ... and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2; Catalog no. H7041; Sigma, St. Louis, MO). All the cells were incubated at 37°C in the presence of 5% CO2.
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bioRxiv - Microbiology 2022Quote: ... Cells were infected at low MOI 1-2 in EBM-2 media with supplements in the presence of 8 μg/mL polybrene (Sigma-Aldrich, St.Louis, USA) and spinoculation at 450g for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... 1 hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in FACS buffer (1x DPBS, 2% FCS and 2 mM EDTA) + 5 μM Actinomycin D (ActD, Sigma, Cat#A1410-5MG) for transcriptomics ...
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bioRxiv - Biochemistry 2022Quote: ... Cytidine 3′, 5′ cyclic monophosphate (3′, 5′ cCMP) and Cytidine 2′, 3′ cyclic monophosphate (2′, 3′ cCMP) were purchased from Sigma-Aldrich (Bangalore, India) and used without further purification ...
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bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
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bioRxiv - Microbiology 2023Quote: hPCLS were infected with SARS-CoV-2 at 2×105 TCID50 per slice and treated with homoharringtonine (HHT) (Sigma, cat# SML-1091-10MG) at 1 h p.i ...
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bioRxiv - Biophysics 2023Quote: Agarose gels were prepared at 2% (weight) in Tris-Borate-EDTA (TBE, Sigma-Aldrich, 89 mM Tris-borate, 2 mM EDTA, pH 8.3) buffer ...
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bioRxiv - Genomics 2023Quote: ... the following modifications were made to the initial extraction: 2% (w/v) polyvinylpyrrolidone (PVP, average molecular weight 40,000) and 10% 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO) were added to the lysis buffer ...
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bioRxiv - Physiology 2024Quote: ... 8.0x104 cells/well were seeded the night prior on a 96-well plate and treated with 5mM 2-deoxy-D-glucose 2h prior to the initial seahorse reading (2-DG; Millipore Sigma cat. D2134). ATP production rates from glycolysis (glycoATP ...
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bioRxiv - Developmental Biology 2024Quote: ... The following chemical compounds were prepared and used according to the manufacturers’ guidelines: 2-deoxy-D-glucose (2-DG, Sigma, cat. no. D8375); oligomycin-A (Sigma ...
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bioRxiv - Biophysics 2024Quote: Saturated salt solutions were prepared by adding Mg(NO3)2·6 H2O and Co(NO3)2·6 H2O (both from Sigma-Aldrich Sweden AB) to H2O (Milli-Q quality ...
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bioRxiv - Plant Biology 2020Quote: ... The anti-GFP antibodies (Sigma, F1804) were immunoprecipitated overnight at 4°C ...
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Building a robust chromatin immunoprecipitation (ChIP) method with substantially improved efficiencybioRxiv - Plant Biology 2020Quote: ... anti-FLAG antibody (Sigma-Aldrich, SAB4301135); anti-GFP antibody(Sigma-Aldrich ...
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Building a robust chromatin immunoprecipitation (ChIP) method with substantially improved efficiencybioRxiv - Plant Biology 2020Quote: ... anti-GFP antibody(Sigma-Aldrich, SAB4301138); protein A/G Agarose beads (Beyotime Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-IgG antibody (Sigma, I5006) overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... anti-polyHistidine−alkaline phosphatase antibody (Sigma). High capacity streptavidin agarose beads (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-Flag antibody (Sigma, F3165)
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase coupled secondary antibodies (Sigma) and Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-mouse monoclonal antibody (Sigma-Aldrich) was used at 1:5000 for western blotting and at 1:500 for immunofluorescence ...
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bioRxiv - Cell Biology 2020Quote: ... anti-HA antibody (12CA5; Roche/Sigma) was used to detect Mps2-3HA ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Flag antibody (Sigma #F3165) 1:1000 ...
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bioRxiv - Immunology 2021Quote: Antibodies against talin (8d4, Sigma-Aldrich) and Flag (M2 ...
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bioRxiv - Developmental Biology 2021Quote: ... We used -tubulin antibodies (Sigma-Aldrich, USA ...
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bioRxiv - Biophysics 2021Quote: ... or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Molecular Biology 2021Quote: Antibodies against FLAG (F1804, Sigma Aldrich), IPMK (custom rabbit polyclonal antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... Monoclonal anti-polyhistidine peroxidase antibodies (Sigma) were used at a final concentration of 1/10000 to specifically detect the his-tagged protein ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-actin antibody (#A2066; Sigma-Aldrich) was used as loading control ...
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bioRxiv - Developmental Biology 2021Quote: ... antibody produced in goats (A0545 -Sigma® ...
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bioRxiv - Developmental Biology 2021Quote: ... antibody produced in goats (A9044 -Sigma® ...
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bioRxiv - Developmental Biology 2020Quote: ... An α-HA antibody 3F10 (Sigma) was added for another 4 hours (1:1,000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-FLAG antibody (Sigma F-1804) was used to detect the 3xFLAG tagged HOXB1 protein in the eluted samples.
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bioRxiv - Biochemistry 2022Quote: ... primary (Sigma anti-Flag M2 antibody) and secondary antibody (Invitrogen anti-mouse antibody ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibody (MX-A: Millipore Sigma, MABF938 ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies against FLAG (Sigma-Aldrich, #F7425), Rab7a (# NBP1-87174 ...