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Citations for Millipore Sigma :
6251 - 6300 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The labeled protein concentration was measured using the Direct Detect spectrometer (Merck Millipore). The degree of labeling was calculated as the ratio of the dye concentration and the protein concentration ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were transferred onto an Immobilon FL PVDF membrane (Merck Millipore, Darmstadt, Germany), probed with antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatants containing chromatin fragments were pre-cleared with protein A agarose beads (Millipore) for 20 min and incubated with a specific antibody overnight at 4 °C on a nutator (antibody information were listed in Supplementary Table 1).then added 50 ul of protein A agarose beads and incubated for 2 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Completeness of protein transfer was determined by Ponceau S (Sigma-Aldrich, cat# P7170) staining for 5-6 min followed by frequent washing with H20 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were immunoprecipitated by incubating cell extracts overnight with either FLAG M2 (Sigma) or GFP (Clonetech ...
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bioRxiv - Cell Biology 2019Quote: ... Total extracted proteins (20 μg) were derivatized according to the manufacturer’s instructions (Millipore). 2-mercaptoethanol was used to reduce samples ...
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bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
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bioRxiv - Cell Biology 2020Quote: Protein loading quantity was controlled using a monoclonal anti-α-tubulin antibody (Sigma). Secondary antibodies were from Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then transferred to Immobilon-P transfer membranes (PVDFs) (IPVH00010; Millipore). The membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies against the following proteins were used: actin (rabbit, Sigma-Aldrich, #A2066), cMyc (mouse ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μl lambda protein phosphatase (800 U) and buffer components as instructed (Sigma) and the reaction was terminated after 30 min at 30 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 300µg of proteins were incubated with 30µl of Streptavidin-Agarose beads (Sigma, CS1638) on a rotating wheel at 4°C for 3hr ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins samples were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore) for Western blot analysis.
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bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
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bioRxiv - Genetics 2021Quote: ... 1 mM phenylmethylsulfonyl (PMSF) and 1% cocktail protein protease and phosphatase inhibitors (Sigma), pH 8.0) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was mixed with 30 μL of protein A Sepharose (Sigma P3391) and rotated for 2 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... inner-membrane proteins were solubilized using 0.5% (w/v) N-lauryl sarcosine (Sigma) in a buffer containing 20 mM Tris-HCl [pH 7.5] and 150 Mm NaCl for 1.5 h at 4 °C on a roller ...
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bioRxiv - Immunology 2021Quote: ... and filtering through 0.65 µm low protein binding filter plates (Millipore, Burlington, USA).
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bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
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bioRxiv - Developmental Biology 2021Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore, Burlington, MA) in transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Priming was done with 50μg of protein in complete Freund’s adjuvant (Sigma, India) per mouse ...
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bioRxiv - Developmental Biology 2020Quote: ... 50μL of pre washed Magna ChIP protein A+G magnetic beads (Millipore Sigma) were then added to the samples and rotated at 4°C for 2 hours to elute the chromatin:antibody complexes ...
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bioRxiv - Immunology 2022Quote: ... were coated with 20μg/ml OVA protein (Sigma Aldrich, St Louis, MO, USA) or 4μg/ml CII (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
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bioRxiv - Biophysics 2022Quote: ... gels were stained for 1 h using ReadyBlueTM Protein Gel Stain (Sigma-Aldrich) and destained with ddH2O overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted by boiling with Sample Buffer Laemli 2X (Sigma-Aldrich) for 5 min at 95°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The captured DNA-RNA hybrids were enriched by protein A-agarose beads (Millipore). The purified DNA was used to construct a library with the NEXTflex Rapid DNA-seq Kit (PerkinElmer).
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bioRxiv - Molecular Biology 2020Quote: ... the protein is transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore Corp., MA, USA) at 20 V ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluates were incubated with 40 μl of S-protein-agarose beads (Millipore) for 2 hr at 4 °C and then washed three times with NETN buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on 6% polyacrylamide gel and transferred on PVDF membrane (Millipore) using semi-dry blotting ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing protein were concentrated in 50-kDa-cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at -80 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Eluted proteins were buffer exchanged and concentrated with PBS using Amicon tubes (Millipore). The proteins were further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 125 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Supernatants were precleared with 20 μL of protein A/G magnetic beads (Millipore) at 4°C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... the protein content and purity were evaluated with SDS-PAGE and InstantBlue (Sigma) gel staining (Extended Data Fig ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then visualized with ProteoSilver Plus Silver Stain Kit (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore) by ultracentrifugation and purity was controlled by SDS-PAGE.
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bioRxiv - Cancer Biology 2019Quote: ... The respective protein bands were visualized using the immunobilion chemiluminescence system (Millipore, MA). The densitometry analysis of immunoblotting images was performed using Quantity One Software (Bio-rad) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein levels were normalized to β-actin (1:5000 dilution, A5060, Sigma Aldrich). Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were precleared by a 30 minute-incubation with Protein G agarose (Sigma), and then incubated for 2 hours at 4°C with Protein G agarose and 5 µg of indicated antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... and the protein concentration was assessed using a BCA kit (Sigma-Aldrich, USA). Samples were diluted to equal concentrations and separated using the Novex Native PAGE Bis–Tris Gel system (4–16% ...
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bioRxiv - Immunology 2019Quote: ... Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore, Watford, UK) visualized on Hyperfilm™ (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Immunology 2019Quote: ... SDS-PAGE separated proteins were transferred onto Immobilon-FL PVDF membranes (EMD Millipore) at 100V for 1h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore cat#WBKLS0500) [7].
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bioRxiv - Biophysics 2020Quote: ... and 10 μL of 5 mg∙mL−1 of protein A (Sigma-Aldrich), were added to 1 mL of 10 mg∙mL−1 of N-(3-dimethylaminopropyl)-N’-ethylcabodiimidie hydrochloride (EDC ...
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bioRxiv - Immunology 2020Quote: Protein lysates were subjected to SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked and probed with one of the following antibodies ...
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bioRxiv - Bioengineering 2019Quote: ... Antibodies were purified from medium using protein G gravitrap (Sigma, 28-9852-55) according to manufacturer protocol ...
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bioRxiv - Microbiology 2019Quote: ... proteins precipitated on the beads were eluted using Laemmli’s buffer containing dithiothreitol (Sigma) boiled 5 min at 95 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... protein synthesis was inhibited by treating cells with 350 µM cycloheximide (Sigma Aldrich).