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Citations for Millipore Sigma :
6201 - 6250 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the expressed protein were combined and concentrated to 2.5 mL with a 30-kDa cut-off centrifugal filter (Millipore), run through the desalting column again ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated by 7.5% SDS-PAGE and transferred to an Immobilon-P membrane (Millipore, Billerica, MA, USA), as previously described (Towbin et al. ...
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bioRxiv - Cell Biology 2024Quote: 500 μg of crude cell lysate was precleared with 10 μl of PureProteome Protein A Magnetic Beads (Merck Millipore, Burlington ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates from the adipose tissue and serum samples were analyzed for inflammatory markers (Millipore Sigma #MCYTOMAG-70K-PMX) and adipokines (Millipore Sigma #MADMAG-71K-07 ...
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bioRxiv - Neuroscience 2024Quote: ... The eluted protein and the beads were separated by briefly spinning in a SpinX column tubes (Sigma CLS8162-24EA). The purity of purified protein was by running 5 µg of protein on an SDS PAGE gel and performing Comassie stain ...
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bioRxiv - Plant Biology 2019Quote: ... The purified protein was concentrated using an Amicon Ultra centrifugal filter with a molecular mass cut-off (MWCO) of 30 kDa (Millipore) and analysed on a Superdex 200 size-exclusion chromatography (SEC ...
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bioRxiv - Synthetic Biology 2021Quote: ... purified Sx-Cm-MOMP and Sx-CtE-MOMP proteins were spotted directly onto nitrocellulose membranes and incubated for 5 min before being blocked with 5% BSA (Sigma) and probing with the same primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... The initial rates of reaction were normalised to total protein content as determined using the QuantiPro BCA Assay Kit (Sigma). Control reactions without any substrate (G3P for GapA and GapN ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μl of Protein G magnetic beads were used and 10 μg of mouse monoclonal anti-FLAG primary antibody M2 (Sigma) were added before overnight incubation at 4°C with the lysate ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg of protein per lysate were subjected to anti-FLAG immunoprecipitation using 15 ul of packed magnetic anti-FLAG beads (M2, Sigma) for 4h ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was diluted 10 times and incubated overnight at 4 °C with 20 µL Protein A/G MagnaChIP beads (Millipore) and 8 µg anti-H3K27me3 (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... The GST-His6-tagged forms of LC3B-His12 and GATE-16-His12 proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed in Lysogeny Broth (LB ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared from cell debris by centrifugation prior to adjustment of protein concentrations between the samples and overnight immunoprecipitation at 4° C with pre-equilibrated anti-HA-agarose (Sigma) or anti-c-myc-agarose (Thermo fisher) ...
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bioRxiv - Immunology 2021Quote: ... 0.1% SDS) and precleared with overheard rotation at 4 degrees for two hours using Protein A+G conjugated magnetic beads (Millipore). Chromatin was incubated with primary antibody against mouse p53 (CM5 ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates (750 μg total protein) were precleared with empty agarose beads and then incubated with anti-FLAG-M2 agarose affinity beads (Sigma) with rotation overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of protein lysate were resolved by SDS-PAGE and subsequently transferred onto polyvinylidene difluoride Immobilon-FL membrane (Millipore). Following transfer ...
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bioRxiv - Microbiology 2021Quote: ... The primary immunization was carried out using 450 μg of GST cleaved PfCD protein and an equal volume of Freund’s complete adjuvant (F5881 # Sigma-Aldrich) via the intraperitoneal route ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μg of protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Millipore, Billerica, MA, USA) and transferred onto a polyvinylidene fluoride membrane (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich) followed by SDS-PAGE and western blot detection with anti-streptavidin HRP antibody (Molecular Probes).
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bioRxiv - Microbiology 2019Quote: Tau protein was concentrated in Amicon centrifugal filters 10 kDa MWCO and finally filtered through 100 kDa Amicon filter (Millipore) to remove pre-formed aggregates ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibody-bound chromatin was recovered after incubation for ~7 h at 4°C with Magna ChIP Protein A + G Magnetic Beads (16–663, Millipore). Beads were washed twice with Low salt buffer (0.1% SDS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell, NC, USA) to a final concentration of 0.2 mg/mL protein and 5x SYPRO orange (Sigma-Aldrich). A temperature gradient of 25 to 70°C (heating rate 1°C per min ...
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bioRxiv - Immunology 2020Quote: Mice were immunized subcutaneously at the base of the tail and lower flanks with 50 μg of whole OVA protein (grade V, Sigma) or OVA323–339 (ISQAVHAAHAEINEAGR ...
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bioRxiv - Immunology 2020Quote: ... 1mM EDTA, and protease inhibitors (aprotinin, leupeptin, PMSF) GST-Hhex fusion proteins were purified over glutathione-Sepharose 4B beads (Sigma) and eluted with 10 mM glutathione according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... In the three subsequent boosters the amount of protein used was 25 μg per mouse mixed with incomplete Freund’s adjuvant (Sigma, India). Boosts were given on days 15 and 28 post-priming ...
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing medium was collected 46 and 65 hours post transfection and was filtered through 0.45 μM Millipore low-protein binding filter units (Millipore SCHVU02RE). Viral supernatant was concentrated by first using low speed centrifugation through 100 kDa MW cutoff Millipore Centricon 70 Plus cartridges to condense volume to < 4mL ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ug to 200 ug of protein was run on a 7.5 or 10% SDS-PAGE gel and transferred onto a PVDF membrane (Sigma-Millipore). The membranes were blotted for 1 hr in 1 ...
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bioRxiv - Synthetic Biology 2022Quote: −80 °C glycerol stocks of secreted proteins were thawed in a water bath and proteins were buffer exchanged using 0.5 mL 30 kDA molecular weight cutoff devices from Millipore Sigma at 4 °C into sterile water ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were separated in sodium dodecyl sulfate (SDS)-PAGE and transferred to polyvinylidene fluoride (PVDF) filter membranes (Millipore, USA) for immune-hybridization ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated at 4 °C overnight in the same solution with the primary rabbit anti-OMP (olfactory marker protein; 1:500, O7889 from Sigma). Immunofluorescence was achieved by reaction with the secondary antibody donkey anti-rabbit-488 (A21206 from Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... Equal protein concentrations were resolved via 8% SDS–PAGE and transferred to Immobilon-P polyvinylidene difluoride membrane (Sigma Aldrich; IPVH00010). Antibodies used ...
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bioRxiv - Neuroscience 2021Quote: ... the same amount of total protein extracts were loaded on 10% SDS-PAGE and then stained with Coomassie Blue R-250 (Sigma). Protein expression was quantified by densitometric analysis with the ImageJ Software.
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bioRxiv - Molecular Biology 2020Quote: ... Aliquots of equivalent total protein were separated by polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (EMD, Millipore; Billerica, MA) membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an Amesham Imager 600 (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an Amesham Imager 600 (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µg of protein was incubated overnight at 4°C with primary antibody as indicated (anti-AHCY [MBL RN126PW], anti-HA [Millipore 05-904] ...
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bioRxiv - Molecular Biology 2021Quote: ... the stained band containing the protein sample was excised and de-stained by washing with 50 mM ammonium bicarbonate (Sigma) and 100% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... One mg of protein in a final volume of 500 µl was incubated with 20 µl of anti-flag agarose beads (Sigma) pre-washed with IP buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were then washed 4x with Buffer A containing only 0.1% NP-40 and bound protein was eluted by 3 sequential incubations in PBS containing 0.1% triton x-100 (Sigma-Aldrich) and 0.5mg/ml of 3x FLAG peptide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 2% of the lysate was stored at −20°C to be used as input and the remaining lysate was incubated overnight at 4°C with 50 μl protein G Dynabeads preincubated for at least 4 hr with 2.5 μg of anti-FLAG M2 (Sigma, A8592), H3K27me3 (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: 10 μL samples containing 10 μM protein (TASOR variants or PARP1 catalytic domain) in the presence or absence of benzamide (Sigma) were loaded into glass capillaries (Nanotemper ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was used to detect protein expression using the following antibodies on paraffin sections: SftpC (rabbit, Millipore, AB37086, 1:250), and Ki67 (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Purified protein was then buffer exchanged into phosphate-buffered saline (PBS) by dialysis and concentrated with 10kDa cutoff centrifuge filters (Millipore). For use in BLI ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Genomics 2019Quote: ... 0.04% (v/v) Tween-20) and FLAG-tagged protein were incubated for 1 hr with Anti-FLAG M2 Affinity Gel (Sigma) while rotating ...
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bioRxiv - Immunology 2021Quote: ... an amount of 15 μg of proteins was loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...