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Citations for Millipore Sigma :
6151 - 6200 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cat # 88- 8824-00) then stained for antibodies of interest for 30 min at 4°C (FITC anti-K14, CBL197F from Millipore; PerCP anti-K18 (NB120- 7797, Novus), ITGA6 (PE anti-CD49F BD Cat #555736) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by a 2 h incubation at 37 °C with the respective primary antibody (anti-G3BP2, 1:600 [Proteintech®, 16276-1-AP]; anti-GAPDH-AF488 [Sigma-Aldrich, MAB374-AF488], 1:500). Cells were washed and incubated with secondary antibody (mouse anti-rabbit IgG-CFL 647 ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin, mouse, Sigma-Aldrich A2228 at 1:2000) was incubated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant from this clarification step were incubated with mouse anti-FLAG antibody (Sigma, clone M2 1µg/ml) over night at 4ºC with gentle rocking ...
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bioRxiv - Microbiology 2020Quote: ... Bound domain C was then detected using an HRP-conjugated anti-FLAG antibody (Sigma-Aldrich, St. Louis, MO) and Ultra-TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were incubated with TBST-Blotto containing primary monoclonal mouse anti-FLAG M2 antibody (1:2,000 dilution, Sigma) or monoclonal mouse anti-GAPDH antibody (1:2,000 dilution ...
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bioRxiv - Systems Biology 2020Quote: ... samples were analyzed for β-actin expression using a mouse anti-β-actin antibody (1:5,000, Sigma Aldrich) and a HRP-labeled goat anti-mouse antibody (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... the localization and the expression level of the construct was assessed by immunofluorescence using anti-FLAG antibody (Sigma). Expression of the construct was induced or not by addition of 1 μg/ml tetracycline (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... The following primary antibodies were used for staining: rabbit anti-SFTPC (Cat#AB3786; Millipore Sigma; used 1:2000), chicken anti-KRT5 (Cat#905901 ...
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bioRxiv - Cell Biology 2020Quote: ... 1:500) and rabbit polyclonal anti-phosphorylated histone H3 (Ser10) (06570, 1:500) antibodies were obtained from Millipore. Monoclonal rat anti-Miranda antibody (ab197788 ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were reprobed with a mouse monoclonal antibody anti-β-actin (clone C4, Millipore; 1:5,000 dilution).
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated in a primary antibody solution containing 1:250 mouse anti-NeuN (Millipore; MA, USA), 1:200 goat anti-DCX(Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were probed with rat anti-HA antibodies (1:100 to 1:3,000 dilutions; clone 3F10, Sigma, 11867423001), rabbit anti-TgTom40 [44] (1:2,000 dilution) ...
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bioRxiv - Molecular Biology 2021Quote: ... Total cell extracts were then processed for western blot using an anti-puromycine antibody (Millipore, clone 12D10 #MABE343) as well as Anti-CDK9 (C12F7 ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then incubated with anti-FLAG M2 primary antibody (1:500 dilution; Millipore Sigma; Cat #: MF1804) in PBS/0.1%Tween20/5% Blotting-Grade Blocker overnight ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Secondary antibodies (1:2500 dilution of goat anti- rabbit IgG conjugated to HRP; Sigma; catalogue number A0545-1ML) were then added to membrane in TBS with 3% BSA and incubated at room temperature on a rocking platform for 2-4 hours ...
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bioRxiv - Systems Biology 2020Quote: ... secondary antibody was added (Goat anti-rabbit HRP, 1% milk, TBST 0.2%, 2 h, RT, 1:5000; Sigma). Once washed ...
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bioRxiv - Developmental Biology 2020Quote: ... The following antibodies were used: mouse anti-acetylated tubulin (clone 6-11b-1, Sigma-Aldrich, diluted 1:2000) and goat anti-mouse Alexafluor 488 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... and a mouse anti-acetylated α-tubulin antibody (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) to co-stain cell boundaries ...
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bioRxiv - Developmental Biology 2022Quote: ... and a mouse anti-acetylated α-tubulin antibody (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) to co-stain cell boundaries ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with HRP-linked secondary antibodies anti-mouse IgG (1:5000, A6782, Sigma-Aldrich, MO, USA) and anti-rabbit IgG (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... protein extracts from SCN cultures were examined by SDS-PAGE immunoblotted with anti-UBE3A monoclonal antibody (E8655, Sigma), or anti-FLAG monoclonal antibody (F1804 ...
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bioRxiv - Molecular Biology 2021Quote: ... plates were incubated with a 1:20,000 dilution of anti-FLAG M2 HRP-conjugated monoclonal antibody (Sigma-Aldrich) in blocking buffer for another 1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... HRPA-1 was detected using the Monoclonal ANTI-FLAG M2-Peroxidase (HRP) antibody (A8592-2MG) from Sigma-Aldrich at a 1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Sections where incubated overnight in blocking solution containing the primary antibodies (Goat anti-ChAT 1:100, Millipore, AB144P). Sections were rinsed in PBS then incubated for 2 hours at room temperature in blocking solution containing secondary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Don3*-Gfp or Jps1-Gfp a primary anti-Gfp antibody was used (1:4000, Millipore/Sigma, Billerica, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2021Quote: ... SHH-tagged Gus-Cts1 was detected using a primary anti-HA antibody (1:4000, Millipore/Sigma, Billerica, USA). For detection of Gfp-tagged proteins like Don3-Gfp ...
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bioRxiv - Molecular Biology 2021Quote: ... Don3*-Gfp or Jps1-Gfp a primary anti-Gfp antibody was used (1:4000, Millipore/Sigma, Billerica, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2021Quote: ... SHH-tagged Gus-Cts1 was detected using a primary anti-HA antibody (1:4000, Millipore/Sigma, Billerica, USA). For detection of Gfp-tagged proteins like Don3-Gfp ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin fractions were sonicated and immunoprecipitated by Anti-FLAG M2 Magnetic Beads affinity isolated antibody (Sigma, no. M8823). Fragment DNA without immunoprecipitation (input DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein levels of wild-type or mutant Rad6 were determined by immunoblotting using anti-Flag M2 antibody (Sigma).
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bioRxiv - Immunology 2019Quote: ... for 30 min and incubated with an anti-GXM antibody (1:100 dilution; EMD Millipore; cat. no. MABF2069) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: The primary antibodies used (with their respective working dilutions) were: anti-FLAG (Sigma F1804, mouse, WB: 1/1000), anti-pan-acetyl lysine (AcK ...
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bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were used: Monoclonal mouse anti-FLAG M2-Peroxidase (HRP) (Sigma-Aldrich; A8592, dilution: 1:1,000). Monoclonal mouse anti-Actin clone C4 (Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies were used for western blotting studies: mouse anti-α-tubulin DM1A (Sigma-Aldrich, RRID:AB_477593) used at 1:10000 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were probed at 4°C with primary antibodies: polyclonal anti-UCP1 (1:500, Sigma-Aldrich cat#U6382), and anti-actin (1:10000 ...
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bioRxiv - Genetics 2019Quote: ... The blots were incubated with rabbit anti-acetyl-histone H3 (Lys14) antibodies (1:1,000 dilution, Millipore, 07-353) and then with horseradish peroxidase-linked donkey anti-rabbit IgG (NA934 ...
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bioRxiv - Biochemistry 2019Quote: ... Western blotting for ADP-ribosylation was performed using anti-pan-ADP-ribose antibody (MABE1016, lot # 3074935, EMD Millipore) as described earlier with minor modifications.11 Briefly ...
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bioRxiv - Immunology 2019Quote: ... IgM was purified using affinity chromatography with polyclonal anti-μ antibody coupled to agarose (A9935, SIGMA-ALDRICH, USA). A 7-mer random peptide library (E8100S ...
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bioRxiv - Microbiology 2019Quote: ... The coverslips were then incubated with a 1:5,000 dilution of mouse monoclonal M2 anti-FLAG antibody (Sigma) and a 1:10,000 dilution of rabbit polyclonal anti-S ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed in PBS and incubated with goat anti-mouse secondary antibody (1:200, Sigma-Aldrich) for one hour at room temperature on a shaker ...
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bioRxiv - Pathology 2020Quote: ... and immunostained with human monoclonal anti-CRP antibody (C1688; Sigma-Aldrich, Saint Louis, MO, USA; 1:400 dilution) which specifically detect the 24-kD monomeric CRP epitope[31 ...
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bioRxiv - Molecular Biology 2019Quote: ... transferred to PVDF membranes and probed with the following primary antibodies: anti-FLAG HRP (1:300, Sigma, A8592), anti-GAPDH (1:1200 ...
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bioRxiv - Microbiology 2019Quote: ... at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2, Millipore Sigma #04-263) at a dilution of 1:400 for 3 hours at room temperature.
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bioRxiv - Biochemistry 2020Quote: ... Lamin proteins were detected using mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR) ...
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bioRxiv - Neuroscience 2020Quote: ... It was then incubated in primary antibodies against GABA and NeuN (rabbit anti-GABA, 1:500, A2052 Sigma; guinea pig anti-NeuN ...
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bioRxiv - Genomics 2020Quote: ... incubated with 1:200 diluted anti-LINE-1 ORF1p mouse monoclonal antibody (clone 4H1, Sigma MABC1152, Lot 3493991), and then with 1:400 diluted Donkey-anti-Mouse-Alexa Fluor 594 second antibody (Invitrogen 21203).
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were labeled by incubation with 1:1000 dilution of AlexaFluor488-conjugated anti-NeuN antibody (MAB377X, Millipore) and a 1:1000 dilution of Hoechst 33342 at 4°C for 1 hour with continuous shaking ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated at 4°C with a rabbit anti-TH polyclonal antibody (1:100; EMD Millipore, RRID: AB_390204). Slices were then washed thoroughly in PBS with 0.2% BSA before being agitated overnight at 4°C with Cy5 anti-rabbit secondary antibody (1:100 ...