Labshake search
Citations for Millipore Sigma :
6101 - 6150 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore; IPVH00010). After blocking with 5 % skim milk ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
-
bioRxiv - Biochemistry 2022Quote: ... Purified proteins were concentrated using Amicon Ultra® 10,000 MWCO centrifugal filter units (Millipore) or Microsep™ Advance 3kDa (PALL ...
-
bioRxiv - Biochemistry 2022Quote: ... Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore, Bedford, MA, USA). The relative intensity of the blot was measured using the ImageJ software and was normalized by the intensity of β-actin blot.
-
bioRxiv - Molecular Biology 2022Quote: ... Protein samples were subjected to SDS-PAGE and transferred to Immobilon-P membranes (Millipore). Membranes were immunoblotted with the indicated antibodies and developed with ECL (Thermo Scientific) ...
-
bioRxiv - Systems Biology 2022Quote: ... proteins were reduced by addition of 5 mM DTT (Merck Sigma, Cat. No. 43815) or 5 mM TCEP (Merck Sigma ...
-
bioRxiv - Systems Biology 2022Quote: ... proteins were loaded to a filter (Merck-Millipore, Microcon 10 kDa, Cat. No. MRCPRT010) after bead beating and reduced by addition of DTT and alkylated with iodoacetamide ...
-
bioRxiv - Developmental Biology 2022Quote: ... The collected CM was concentrated by protein precipitation with 80%-saturated ammonium sulfate (Sigma) and dissolved in phosphate-buffered saline without calcium and magnesium [PBS (-)] supplemented with 0.1% Triton X-100 and a proteinase inhibitor mixture [0.2 mM 4-(2-aminoethyl ...
-
bioRxiv - Microbiology 2022Quote: ... the respective proteins were overexpressed in BL21 DE3 cells carrying a pET28a vector (Novagen) with an (indirectly ...
-
bioRxiv - Developmental Biology 2022Quote: ... The collected CM was concentrated by protein precipitation with 80%-saturated ammonium sulfate (Sigma) and dissolved in 50 mM Tris-HCl (pH 7.4 ...
-
bioRxiv - Microbiology 2022Quote: ... total protein lysates were instead incubated overnight with Anti-FLAG M2 Magnetic Beads (Sigma) or G-coupled magnetic beads ...
-
bioRxiv - Biochemistry 2022Quote: ... The proteins were then transferred onto a PVDF transfer membrane (Immobilon, Millipore, Bedford, MA). The presence of transferred proteins on the membrane was confirmed by staining with Ponceau S (Sigma) ...
-
bioRxiv - Biochemistry 2022Quote: ... The flow-through containing the target protein was concentrated (Amicon 50 kDa cutoff, Millipore) and loaded onto a size-exclusion column (SRT-3C SEC-300 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins collected from size-exclusion chromatography were concentrated (Concentrators, Amicon Ultra, Millipore, Darmstadt, Germany), aliquoted and directly used for crystallization or stored at −80◦C.
-
bioRxiv - Cell Biology 2022Quote: ... and immunoreactive proteins were visualised using Immobilon® Forte Western HRP Substrate (Merck Millipore). Densitometric analysis was carried out using the ImageJ 1.53c software ...
-
bioRxiv - Biophysics 2022Quote: ... Protein expressions were induced by 1 mM isopropyl β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich) overnight at 25°C ...
-
bioRxiv - Biophysics 2022Quote: ... and NpSxph I559Y) Protein samples were filtered through a 0.22 µm spin filter (Millipore) before loading into the sample cell and titrated with STX (300 µM STX for RcSxph D794 and 100 µM STX for RcSxph ...
-
bioRxiv - Cell Biology 2022Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K MWCO concentrator (EMD Millipore) to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
-
bioRxiv - Cancer Biology 2022Quote: ... The separated proteins were transferred on to 0.45 µm PVDF membrane (Merk Millipore, #IPVH00010), followed by blocking with 5 % skim milk in TBS-T ...
-
bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein concentration for lysates was determined using BCA reagent (Sigma-Aldrich, St. Louis, MO), and equal amounts of protein (50 μg/lane ...
-
bioRxiv - Cell Biology 2022Quote: RIP assay was done with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). Quantitative RT-PCR was used for determining total RNA levels.
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride (PVDF) membranes (Millipore) according to standard protocols ...
-
bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
-
bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... the protein was eluted with sonication buffer containing 50 mM reduced glutathione (Sigma-Aldrich) and dialyzed against storage buffer [50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein bands were developed using Luminata Classico or Luminata Forte HRP substrate (Millipore Sigma) and detected using an Amersham Imager 600 (GE Healthcare ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tiam1 protein was purified using the ANTI-FLAG® M2 affinity gel (Sigma-Aldrich) followed by Superdex 75 ...
-
bioRxiv - Cell Biology 2022Quote: Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
-
bioRxiv - Plant Biology 2021Quote: ... Total protein extracts were incubated with 5 μl T7 antibody (EMD Millipore; Cat # 69522) for 4 h ...
-
TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged proteins were immunoprecipitated using 20 μl M2 beads (Sigma Cat. #A2220) and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... the clear protein lysate was deposited in a Microcon-30kDa filter unit (Millipore, MRCF0R030) and centrifuged at 14,000g for 20 min to pass the totality of the sample through the filter ...
-
bioRxiv - Developmental Biology 2021Quote: ... followed by a 30 min incubation with 30 µl protein-A Sepharose (Sigma- Aldrich). Protein complexes were washed three times by centrifugation (2.700×g ...
-
bioRxiv - Microbiology 2020Quote: ... Test proteins were re-suspended as purified or dialysed with U-tube concentrators (Novagen) into 50 mM Tris-HCl pH 7.5 ...
-
African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... and then pre-washed with 50 μl of protein G agarose bead slurry (Millipore) at 4° C for 2 h ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were stained using primary antibodies against β-actin (1:10,000, AC-15, Sigma), strep II-tag (1:1,000 ...
-
bioRxiv - Molecular Biology 2019Quote: ... the lysate was mixed with 100 µl of protein A-sepharose beads (Sigma-Aldrich), pre-washed five times with 1 ml of 20 mM Tris-HCl ...
-
bioRxiv - Systems Biology 2020Quote: ... Fusion proteins were then expressed in the Rosetta P3 DE LysS E.coli strain (Novagen) using an autoinduction protocol (Jolma et al ...
-
bioRxiv - Molecular Biology 2019Quote: ... was added to H3K27me2/3 ChIP samples and Protein A agarose (40 µL; Sigma) was added to GFP ChIP samples and incubated for 3 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS–PAGE and transferred to PVDF membranes (Immobilon-P; Millipore) in a submerged tank ...
-
bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated with the Quantipro BCA assay kit (Sigma-Alrich, United States), and lysates were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel prior to transfer onto polyvinylidene fluoride membranes ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was then concentrated using a 10 kDa cutoff centrifugal filter unit (Millipore) and chromatographed on a Superdex 200 gel filtration column in a final buffer of 20 mM HEPES pH 7.0 ...
-
bioRxiv - Neuroscience 2021Quote: ... dilute proteins were concentrated using Amicon Ultra 15mL centrifugal filters (Merck Millipore, 10kDa NMWCO) and subjected to single-step buffer exchange into SEC running buffer (see SAXS method ...
-
bioRxiv - Biochemistry 2021Quote: ... and the protein was concentrated using Amicon 3 kDa MWCO centrifugal filters (Merck Millipore). The protein concentration was calculated using the measured absorbance at 280 nm under native conditions and assuming an extinction coefficient of 19940 M-1 cm-1.
-
bioRxiv - Immunology 2020Quote: ... 40 uL of Salmon Sperm DNA/Protein A Agarose-50% (Millipore, Cat #16-157C) for 30 min spinning at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... Supernatant was first incubated with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h to remove background signal ...
-
bioRxiv - Neuroscience 2020Quote: ... 30-40µg of the total protein was combined with 2X sample buffer (Sigma-Aldrich) and boiled for 10 mins before loading in 11% SDS-PAGE ...