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Citations for Millipore Sigma :
6051 - 6100 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The delipidated NPC2 proteins were incubated with increasing concentrations of cholesterol (>99%) (Sigma Aldrich), in DMSO ...
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bioRxiv - Microbiology 2019Quote: ... and the soluble proteins were separated from the OMVs using an iodixanol (Optiprep, Sigma) density gradient ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were resolved by 10% SDS-PAGE and electrotransferred onto polyvinylidene fluoride membrane (Millipore) in transfer buffer (10 mM CAPS ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were then concentrated on either Amicon (Merck Millipore, MWCO 10 kDa) or Vivaspin (Sartorius ...
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bioRxiv - Bioengineering 2020Quote: ... using one of the following extracellular matrix (ECM) protein products: laminin (Laminin 111, Sigma L2020 ...
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bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2020Quote: ... Total protein concentration was measured using bicinchoninic acid assay (BCA Kit, Sigma-Aldrich, USA).
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using Centriprep centrifugal filter devices (Millipore; molecular weight cutoff, 10 kDa) and further purified using size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced using 2.5 μL of dithiothreitol (200 mM DTT stock solution) (Sigma) at 90 °C for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... The immune complexes were incubated with protein A Sepharose beads (Sigma, St. Louis, MS) at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Protein purity was assessed by 20% SDS-PAGE stained with Coomassie Brilliant Blue (Sigma). PageRule Unstained Broad Range Protein Ladder and PageRuler Plus Prestained Protein Ladder (both Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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bioRxiv - Genetics 2021Quote: ... The immune-reactive proteins were detected using the Luminata Crescendo Western HRP substrate (Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted by 1X PBS with 10 mM reduced glutathione (Sigma Aldrich). Fractions containing VCA-NWASP were concentrated and dialyzed to 20 mM Tris (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Biochemistry 2020Quote: Antibiotics used in protein expression and other analytical grade chemicals were purchased from Sigma. Unfractionated heparin was purchased from Sigma as a sodium salt ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... were transiently transfected with the S protein expression construct using polyethylenimine (PEI; Sigma-Aldrich) in FreeStyleMedia (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... Mass inaccuracies were corrected using ProteoMass Peptide and Protein MALDI-MS calibration standard (Sigma). Glycan compositions were manually inspected using MALDI LIFT-TOF/TOF MS/MS.
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bioRxiv - Biochemistry 2020Quote: ... albumin (66 kDa) and carbonic anhydrase (29 kDa) protein molecular weights standards (Sigma Aldrich). Corresponding fractions for each chromatogram between 12 and 18 ml with an interval of 0.5ml were analyzed by SDS-PAGE (Biorad ...
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bioRxiv - Biochemistry 2020Quote: ... Protein visualization was achieved by using Coomassie Brilliant Blue G250 staining (Sigma-Aldrich, Germany) and imaging with a ChemDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were concentrated using Amicon centrifugal filters (5k MWCO or 10k MWCO, Millipore) and purified further by size exclusion chromatography (Superdex 75 Increase ...
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bioRxiv - Neuroscience 2021Quote: ... and those directed against the following proteins: Actin (rabbit, Sigma, A2066, WB 1 : 2000), GFAP (mouse ...
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bioRxiv - Cell Biology 2021Quote: Protein was isolated from cells using 1X RIPA lysis buffer (EMD Millipore, Burlington, MA) containing 1X protease inhibitor cocktail (Thermo-Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested with lysyl endopeptidase (Wako Pure Chemical Industries) and trypsin (Sigma-Aldrich) with an enzyme:substrate ratio of 1:75 at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 µL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Cancer Biology 2020Quote: Protein was extracted from tissue or cells by addition of RIPA Buffer (Sigma Aldrich) including complete Protease Inhibitor Cocktail Tablets (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein lysates were run on SDS-PAGE gels and transferred to PVDF membranes (Millipore). Membranes were blocked and probed with antibodies directed against RAS (RRID:AB_397425 ...
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bioRxiv - Cell Biology 2021Quote: ... the protein sample is transferred to the polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, USA). Blocking the membrane with 5% BSA (Bovine serum albumin) ...
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bioRxiv - Pathology 2021Quote: ... Immunoprecipitated proteins were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). The membranes were probed with rabbit anti SLP2 and mouse anti Flag M2 ...
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bioRxiv - Plant Biology 2021Quote: ... an equal amount of total protein extracts was incubated with anti-GFP (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was dried and resuspended in Protein Extraction Reagent (C0856, Sigma-Aldrich®) supplemented with 1% Protease inhibitor (P9599 ...
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bioRxiv - Cell Biology 2021Quote: ... was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich). PDMS stamps were incubated with solution containing 50μg/ml fibronectin and 1μg/ml of the conjugated fibronectin in Dulbecco’s phosphate-buffered saline (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and the fractionated proteins detected by direct staining of the gel with InstantBlue (Sigma) or by western blot ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were digested on-bead with 1 µg of trypsin (Sigma Aldrich, Cat# 6567) in 50 µL ABC buffer with rotation at 37℃ overnight ...
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bioRxiv - Systems Biology 2020Quote: Proteins from each sample were subjected to reduction using 5 mM DTT (Sigma-Aldrich) at 60°C for 20 min followed by alkylation using 10 mM iodoacetamide (Sigma-Aldrich ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... The equal amount of proteins were incubated with streptavidin-agarose slurry (Millipore, #16-126) at 4°C for 16 h in order to pull down all biotin-labeled proteins ...
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bioRxiv - Microbiology 2021Quote: ... proteins were transferred to Immobilon-PSQ PVDF membrane (0.2 μm pore size, Millipore Sigma) by electrophoresis at 100 V for 30 minutes in Tris-Glycine/Methanol buffer ...
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bioRxiv - Microbiology 2021Quote: ... followed by membrane protein cross-linking with 3,3’-dithiobis(sulfosuccinimidyl propionate) (Sigma; 1 mM), at low temperature ...
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bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
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bioRxiv - Physiology 2021Quote: ... using the following primary antibodies: glial fibrillary acidic protein or anti-GFAP (Millipore, MAB360). Dako Animal Research Kit for mouse primary antibodies (Dako Diagnóstico S.A. ...
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bioRxiv - Microbiology 2021Quote: ... Digested proteins were analyzed by Western blotting with anti-M2 FLAG monoclonal antibody (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed and proteins were detected with Immobilon Western chemiluminescent HRP substrate (Millipore) using the ChemiDoc XRS+ Imaging System.
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bioRxiv - Cancer Biology 2020Quote: ... Protein turnover was analyzed by addition of Emetine (100 μM, Sigma Aldrich, Cat# E2375) and RNA decay by addition of Actinomycin D (5μM ...
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bioRxiv - Cancer Biology 2020Quote: RIP assays employed the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...