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Citations for Millipore Sigma :
6001 - 6050 of 10000+ citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 660 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the blot was probed for HA-epitope (rabbit anti-HA, Cell Signaling #3724, 1:1000; or rat anti-HA, Sigma #11867423001, 1:1000), or PSD95 (mouse anti-PSD95 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416, RRID:AB_258167; anti-rabbit, Sigma-Aldrich Cat# A6154, RRID:AB_258284) (1/2000) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with a biotinylated secondary antibody (anti-mouse: horse, BA-2000, Vector Laboratories, Burlingame, USA; anti-rabbit: goat, B8895, Sigma-Aldrich, USA), diluted 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Neuroscience 2023Quote: ... blocked with 5% FBS and stained overnight at 4°C with primary antibodies for immunocytochemical analysis (rabbit anti-OCT4, 1:100, Proteintech, Cat. no. 11263-1-AP / mouse anti-TRA-1-81, 1:500, Millipore, Cat. no. MAB4381). Samples were visualized after staining with Alexa Fluor 488-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were probed with either rabbit anti-RACK1A (1:500) (Chang et al., 2005) or mouse anti-α-Tubulin (1:3000) (Sigma-Aldrich T6074) antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used: rabbit anti-Myosin7a (Proteus 25-6790; 1:1000) mouse anti-pan-Maguk (IgG1) (Millipore MABN7; 1:500); mouse anti-Ribeyeb (IgG2a ...
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bioRxiv - Immunology 2021Quote: ... The slices were rinsed in TBS-T (5 min each) followed by the addition of secondary antibody (Goat anti-mouse IgG-biotin from Sigma, 1:500) and kept at RT for 2 hours in a humidified chamber ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each and 100 μl of blocking solution containing goat anti-mouse IgG (Sigma 1:3,000) was added to each well and incubated at 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated at 14 °C (a temperature not permissible for recycling) with unconjugated goat anti-rat IgG (Sigma, 4 μg/ml) to block labelled channels remaining at the cell surface ...
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bioRxiv - Biophysics 2020Quote: ... was used as secondary antibody for the microtubule and single molecule surfaces staining and goat anti-mouse IgG (Sigma-Aldrich, SAB3701063-1) was used for β-tubulin immunostaining in FLIM experiments ...
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bioRxiv - Microbiology 2021Quote: ... The grids were rinsed with the same buffer five times and then were incubated with anti-mouse IgG conjugated with 10 nm gold particles (Sigma, cat# G7652) at a 1:10 dilution in 1% BSA-PBS for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Immunology 2020Quote: ... 5 μm sections of frozen post-ISOPROTERENOL hearts were stained with goat anti-mouse IgG-FITC (cat. F5387, Sigma-Aldrich, Dorset, UK), rat anti-mouse IgM-PE (cat ...
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bioRxiv - Plant Biology 2021Quote: ... Immunodetection was performed by incubating the immunoblot for 2 h with anti-mouse IgG conjugated with horseradish peroxidase (1:10,000 dilution; Millipore; Catalog # 12-349). Detection was performed using the SuperSignal West Dura Detection Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The primary antibody used for Western blot was a horseradish peroxidase conjugated goat anti-mouse IgG (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Cell Biology 2020Quote: ... the membranes were washed with TBST for 3 times and incubated for 1 h with a secondary antibody: anti-mouse IgG antibody (HRD) (Sigma-Aldrich, A9044) and anti-rabbit IgG antibody (HRD ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed again 5 times with the washing buffer to discard unbound serum and 100 μl of horseradish peroxidase-conjugated goat anti-mouse IgG (Sigma-Aldrich, USA) diluted at 1:2000 in 1% BSA in washing buffer was added to each well followed by 1 hour incubation at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubations with corresponding horseradish peroxidase (HRP)-conjugated goat polyclonal anti-mouse IgG (1:5,000 dilution, EMD Millipore Etobicoke, Ontario, Canada). Blots were developed with Supersignal West Femto enhanced chemiluminescence substrate (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
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bioRxiv - Immunology 2023Quote: ... Non-antigen specific IgG and RBD-specific IgG were isolated from serum and eluted RBD-specific antibodies respectively by protein G beads (Millipore). Purified IgG was denatured and treated with PNGase enzyme (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
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bioRxiv - Genomics 2021Quote: Specific antibodies recognizing human H1 variants used for ChIP/ChIP-seq were: anti-H1.0/H5 clone 3H9 (Millipore, 05-629-I), anti-H1.2 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both cleaved and intact SNAP-25 ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Microbiology 2023Quote: ... Presence or absence of a ZCCHC3 specific band with each clone was determined by western blotting using an anti-human ZCCHC3 antibody (SAB1408147, Sigma-Aldrich).
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bioRxiv - Plant Biology 2021Quote: ... diluted anti-YFP antibody or other specific antibodies followed a 1:10,000 (v/v) diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or a goat anti-mouse antibody (Sigma-Aldrich, St. Louis, MO, USA). The detection signal was developed using the ECL substrate kit as instructed (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... One to three 12 μm sections were chosen randomly from both sides for staining as previously established6 using an anti-CGRP antibody (1:5000, Sigma C8189, anti-rabbit polyclonal), an anti-P2X3 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-phospho-c-Jun (Thr91, cell signal, 2303, 1:1000), rabbit anti-c-Jun (cell signal, 9165, 1:1000) and mouse anti-GAPDH (Sigma, Cat: G8795, 1:1000). Immunoreactivity was visualized using appropriate HRP-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... and rabbit anti-pSer502-NT5C2 (dilution 1:1000) (Covance) antibodies and β-actin with a mouse monoclonal anti-β-actin antibody (Sigma Aldrich, #A5441). We visualized the immunoblots using an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Genomics 2022Quote: ... (D5E4) XP® Rabbit mAb, CST, Cat #8173S), anti-ETS-1 (C-20, SantaCruz, Cat# sc-350X) and anti-CTCF (Millipore, Cat# 07-729), along with positive and negative controls ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed with TBST buffer (three times, 7 minutes each) and incubated with anti-mouse-HRP or anti-rabbit-HRP (Sigma-Aldrich; 20,000 times dilution) for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Sigma-Aldrich, A9044 and A5278), followed by incubation with the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... tissue sections were exposed to anti-Iba1 rabbit antibody (FUJIFILM Wacko, 013-27691, 1:300 dilution) and anti-GFAP mouse antibody (Sigma, G3893, 1:300 dilution) for overnight at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T). The intensity of the signal from retained anti-AGO1-N-coil antibody was quantified using Image J and fitted using Nonlinear Least Square (NLS ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Acetylated histone H4 and H2A were detected by immunoblotting with rabbit anti-acH4 (UBI, Catalog # 06–866, 1:1000 dilution) and anti-acH2A K9 (Millipore Sigma, Catalog # 07-289), respectively.
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bioRxiv - Neuroscience 2024Quote: ... the first incubation was performed with mouse anti-RFP and rabbit anti-PCP4(respectively: 1:500, Invitrogen, # 710530 and 1:300; Sigma-Aldrich, #HPA005792, RRID: AB_1855086). The secondary incubation was performed with anti-mouse conjugated to Alexa 488 and anti-rabbit IgG2a conjugated to Alexa 568 (respectively ...
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bioRxiv - Cell Biology 2020Quote: ... or 10 µg of mouse IgG (Sigma-Aldrich) in 200 µl of PBS + 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... Monoclonal IgG directed against UBE3A protein (E8655, Sigma) and anti-β-ACTIN monoclonal antibody (A5316 ...