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Citations for Millipore Sigma :
5951 - 6000 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Captured protein was detected by immunoblotting with anti-FLAG M2 antibody (Sigma) and visualized using an Odyssey Infrared Imaging System (Licor) ...
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bioRxiv - Biochemistry 2024Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The pooled protein was concentrated using a centrifugal concentrator (Millipore, 100 kDa) prior to injection onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... A mixture consisting of rCas9NLS protein (0.66 mg/ml, Sigma-Aldrich CAS9PL), Phenol red (0.05% ...
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bioRxiv - Bioengineering 2024Quote: ... One hundred µL of protein samples or lysostaphin standards (SIGMA-L9043-5MG) diluted in 0.01% Tween 80 (SIGMA-P5188-100ML ...
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bioRxiv - Cancer Biology 2024Quote: ... equal amounts of protein were run and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 5 % milk and incubated with the following antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by BCA assay (Sigma Aldrich, cat no: B9643). 30-40 μg protein was loaded in Mini-PROTEAN® Tetra Cell Systems and TGX™ precast gels ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended cells were supplemented with Bug Buster protein reagent (Millipore #70584-3) before being sonicated ...
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bioRxiv - Cancer Biology 2024Quote: ... as per manufacturer instructions and 0-5µg/mL OVA protein (Millipore Sigma) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The separated proteins were transferred to a PVDF membrane (Millipore, 0.2 μm). The membrane was blocked in 5% nonfat dry milk (Biofroxx ...
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bioRxiv - Molecular Biology 2024Quote: ... Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF) membrane (Millipore) for immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore) and blocked with Intercept Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was labeled using an atto-488-NHS ester (Sigma Aldrich 41698) and then dialyzed again to remove unconjugated dye ...
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bioRxiv - Bioengineering 2024Quote: ... Protein expression was then induced by addition of 1M IPTG (Sigma-Aldrich), before harvesting of cells after 8 hours ...
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bioRxiv - Immunology 2023Quote: ... H3K27me3 or HK3K27Ac ChIP-grade antibodies and Protein A magnetic beads (Millipore). FAIRE was performed on samples fixed and sonicated as per ChIP ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with 1:50 trypsin (Sigma-Aldrich) and 1:75 and lysyl endopeptidase (Wako) ...
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bioRxiv - Genetics 2023Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-FL, Millipore, Burlington, MA) and blocked for 1 h at room temperature in Odyssey blocking buffer (Li-Cor ...
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bioRxiv - Biochemistry 2023Quote: ... Antibody precipitated with 100 µL Protein A/G beads (Millipore, IP05-1.5ML) was washed twice in 700 µL DRIP buffer ...
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bioRxiv - Microbiology 2023Quote: ... followed by the transfer of protein onto the PVDF membrane (Merck Millipore) using a wet-tank blotting system (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then transferred to PVDF membrane (Immobilon, Millipore, Billerica, MA, USA) by semi-dry blotting ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were reduced with 10mM tris(2-carboxyethyl)-phosphine (TCEP) (Sigma) for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504). Blots were blocked (5% (w/v ...
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bioRxiv - Physiology 2023Quote: ... Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500, Millipore), visualized using ChemiDoc Touch Imaging System (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... then stained with ReadyBlue™ Protein Gel Stain from Sigma-Aldrich (USA) and imaged and analyzed.
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bioRxiv - Molecular Biology 2022Quote: Total proteins were extracted from cells using RIPA buffer (Sigma-Aldrich, #R0278) and quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies used for probing for specific proteins were: actin (Sigma A-2066), Hsp70 (Thermo-Fisher MA 3006) ...
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bioRxiv - Cell Biology 2023Quote: ... Rab proteins were either loaded with GDP (REP-complexes, Sigma Aldrich, Germany) or MANT-GDP (GDI-complexes ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% dry milk in TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... protein lysates were separated and transferred onto polyvinylidene fluoride membranes (Millipore, USA). After being blocked with five-percent non-fat dry milk in Tris-buffered saline Tween-20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... All lysed protein samples were incubated with anti-Flag magnetic beads (Sigma) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504) before being scanned on a flatbed scanner (Epson V800 Photo) ...
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bioRxiv - Cancer Biology 2023Quote: Immunoprecipitation was performed using Protein-A Sepharose from Staphylococcus aureus (Merck Millipore) and anti-TKS4 antibody (Lányi et al ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellet was washed with −20°C cold acetone (Sigma-Aldrich), vortexed ...
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bioRxiv - Cancer Biology 2023Quote: ... protein samples were prepared with sample buffer that contains 10% glycerol (Sigma) and 5% 2-mercaptoethanol (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentration was measured using a Bradford assay (Sigma France, Lezennes, France). For SDS-PAGE analysis of purified proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Millipore, IPVH00010). The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and the protein pellet resuspended and incubated in 2M hydroxylamine (NH2OH) (Sigma), pH 7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were incubated with 20 μl protein G-coated Sepharose (Sigma-Aldrich) previously conjugated with 5 μl of anti-FLAG antibody by overnight incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore) overnight at 4°C and blocked in either 5% nonfat powdered milk or 5% bovine serum albumin in TBS with Tween 20 (pH 7.6 ...
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bioRxiv - Plant Biology 2023Quote: ... Crude protein concentration was determined using the Bradford Reagent (Sigma-Aldrich, MO), following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was qualified by Bradford assays (Sigma-Aldrich, B6916-500 mL). 30 μg soluble proteins were resolved on a 15% sodium dodecyl sulphate poly acrylamide gel via electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... equal amounts of proteins were then incubated with anti-Flag Beads (Sigma), anti-Myc Beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Protein detection were performed with Immobilon Western Chemiluminescent HRP Substrate (ECL, Millipore). Band intensities were analyzed by ImageJ software.
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bioRxiv - Neuroscience 2023Quote: ... 25μg protein lysate were resuspended in Laemmli buffer 1X (Sigma, S3401-1VL), denatured at 95°C for 5 minutes and loaded onto a polyacrylamide gel (Mini-PROTEAN(R ...
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bioRxiv - Cell Biology 2023Quote: ... Separated proteins were then electroblotted onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). Following the transfer ...