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Citations for Millipore Sigma :
5951 - 6000 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... immediately followed by a coating of 3- (trimethoxysilyl)propyl methacrylate (TMS) (Sigma-Aldrich, USA) to methacrylate the glass surfaces (Guvendiren et al. ...
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bioRxiv - Neuroscience 2021Quote: Tadpoles were briefly anesthetized with 0.1% MS-222 (ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich), and subsequently immobilized by immersion in a 10 μM α-bungarotoxin (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide] dye solution (Sigma-Aldrich, USA) (5 mg MTT in 1 ml PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 500 μl PBS (PBST) containing 3% normal donkey serum (S30-100ML, Millipore, Hertfordshire, UK) at room temperature (RT ...
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bioRxiv - Physiology 2021Quote: ... rAAVs were washed 3 times with PBS using Amicon Ultra Centrifugal Filter Units (Millipore) and resuspended in PBS + 0.001% Pluronic F68 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed 3 times in PBS and fixed in 4% paraformaldehyde (Sigma Alrich) PBS for 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: Bonner’s Salts was used to make the solutions of 3 μM Ionomycin (Sigma, I0634) and DMSO carrier control ...
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bioRxiv - Cell Biology 2022Quote: ... Tryptophan and Histidine and containing 50 mM of 3AT (3-amino-1,2,3-triazole, Sigma). Picture of the plates were taken using the Fusion FX6 EDGE Imaging System (Vilber ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... then 85℃ for 10 minutes and transfected in a 3:1 polyethylenimine (PEI, Sigma):DNA ratio into 70% confluent HEK 293A cells (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... blocked for 3 hours in PBS containing 10 % normal goat serum (NGS) (Sigma-Aldrich), before incubation with anti-GluA2 (RRID:AB_10761960 ...
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bioRxiv - Microbiology 2019Quote: ... before being concentrated using Amicon Ultra-2 centrifugal 3 kDa filter units (Millipore Sigma). For Western blot analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 mM 8-bromoadenosine 3□,5□-cyclic monophosphate (Sigma-Aldrich, Saint Louis, MO, USA), 1 μM medroxyprogesterone acetate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... 2’-O-dibutyryladenosine 3’,5’-cyclic monophosphate sodium salt (db-cAMP; 1 mM; Sigma). Media was replaced every 2-3 days for a minimum of 15 days ...
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bioRxiv - Biochemistry 2019Quote: ... and csm5 were cloned into pACYCDuet-1 (5’-NcoI, 3’-XhoI; Novagen, Merck Millipore), csm4 was cloned into pEHisTEV (5’-NcoI ...
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bioRxiv - Biochemistry 2019Quote: ... and csm5 were cloned into pACYCDuet-1 (5’-NcoI, 3’-XhoI; Novagen, Merck Millipore), csm4 was cloned into pEHisTEV (5’-NcoI ...
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bioRxiv - Cell Biology 2019Quote: ... concentrated with an Amicon Ultra-15 centrifugal 3 kDa MWCO filter (Millipore Cat# UFC900324), endotoxin purified using a Pierce high capacity endotoxin removal spin column (ThermoFisher Cat# 88274) ...
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bioRxiv - Molecular Biology 2019Quote: ... then incubated in 3 mL of 0.025% trypsin-EDTA solution (Sigma Cat No. T4049) for 2-5 min at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... using 3 g/l Phytagel as solidifying agent (Sigma-Aldrich®, St. Louis, USA.) and then incubated under 16L:8D photoperiod at 27 °C.
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bioRxiv - Systems Biology 2019Quote: ... Sodium bicarbonate and sodium borohydride were purchased from EMD Millipore (#SX0320-1, #SX0380-3). mPEG-Succinimidyl Valerate (MPEG-SVA-5000-5g ...
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bioRxiv - Cell Biology 2019Quote: ... 3-isobutyl-1-methylxanthine (I5879) and 2’,5’-dideoxyadenosine (D7408) were purchased from Sigma. Carvedilol (15418 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were incubated overnight and then selected by puromycin (3 μg/mL, Sigma-Aldrich). Cells were passaged and cultured under puromycin selection for at least 5 days before flow cytometry analysis using an LSRFortessa X20 instrument (BD Biosciences).
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bioRxiv - Biophysics 2019Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647N (Atto 647N DOPE) (Sigma) was incorporated into all lipid bilayers for visualization ...
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bioRxiv - Cancer Biology 2019Quote: ... Endogenous peroxidase activity was blocked by incubating the slides in 3% wv H2O2 (Sigma). The blocking buffer contains 0.2% goat serum ...
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Obesity alters mobility and adult neurogenesis, but not hippocampal dependent learning in ob/ob micebioRxiv - Neuroscience 2019Quote: ... the solution containing the primary rabbit anti-active Caspase 3 antibody (Merck Millipore, Germany) was applied 1:100 in blocking serum (3 % NGS + 0.1 % Triton X-100 in PBS ...
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bioRxiv - Microbiology 2019Quote: ... 3-(N-Morpholino) propanesulfonic acid (MOPS) was obtained from Sigma Aldrich (St. Louis, MO). YPD broth medium and YPD agar were obtained from Becton ...
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bioRxiv - Molecular Biology 2019Quote: The plant auxin analog indole-3-acetic acid (IAA) (Sigma, Cat# I3750-5G-A) was dissolved in Ethanol at a stock concentration 500mM and aliquoted to store at −20°C ...
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bioRxiv - Bioengineering 2019Quote: ... A 3% alginate mixture (w/v) was made with alginic acid powder (Sigma-Aldrich) and alpha Minimum Essential Medium (αMEM ...
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bioRxiv - Neuroscience 2019Quote: ... following the manufacturer’s instructions with the exception that 1-Bromo-3-chloropropane (Sigma, B673) was used instead of chloroform.
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bioRxiv - Biochemistry 2019Quote: ... The plate was developed with 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (Sigma), and analysed by spectrophotometry at 405 nm ...
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bioRxiv - Neuroscience 2019Quote: ... After washing (5x 3 min) with deionized water (“MilliQ”, Merck Millipore, Burlington, Massachusetts, USA), sections were placed in an aqueous 1% thiocarbohydrazide solution for 20 min followed by another washing step with deionized water (5x 3 min) ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with primary antibody for (Tuj1, Sigma T5076, 1:500 in PBS+3% BSA), washed with PBS 2 x 1h and 1x overnight ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated for 3 hours at 37°C with 2μM nocodazole (Sigma, M1404). Nocodazole was then washed out by 3 washes with 2ml of pre-warmed PBS1X and one of media at 370C ...
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bioRxiv - Immunology 2019Quote: ... at 28.5°C and treated with 0.02% ethyl 3-aminobenzoate methanesulfonate (Tricaine, SIGMA-ALDRICH) for anesthesia before bacterial injections ...
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bioRxiv - Bioengineering 2019Quote: ... The gold mesh was successively treated with 5% 3-aminopropyl triethoxysilane (APTES) (Sigma-Aldrich) and 0.1% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... 20 μl of 250 μM of folic acid (Millipore Sigma CAS 59-30-3) dissolved in 100 mM NaOH were injected into the wells by a P10 pipette for 20 minutes absorbing by the agar ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: OPC primary cells were treated with JMJD3 inhibitor (GSKJ-4, 3 µM, SML0701, Sigma) for 48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... we added 1 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma-Aldrich, Cat# I7018-100MG) to the cells and incubated them at 37 °C for 30 – 60 min ...
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bioRxiv - Bioengineering 2021Quote: We fixed the cells seeded in hydrogel microwells with a 3% paraformaldehyde (Sigma-Aldrich) for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... These cells were pretreated with PBS containing 3% (w/v) BSA (Sigma-Aldrich Japan) and 10% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... The Mpro fractions were concentrated using 3 kDa Amicon Ultra Centrifugal Filters (Merck Millipore) and the Mpro protein was further purified by size exclusion chromatography using a HiLoad Superdex 200 column (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... the complex was eluted using 0.15 mg/ml 3×FLAG peptide (Millipore Cat# F4799) in GDN buffer ...
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bioRxiv - Neuroscience 2020Quote: ... using gel purified oligoribonucleotide probes synthesized with both 5′ and 3′ fluorescein modifications (Sigma). Fluorescein labeled probes were detected using an alkaline-phosphatase conjugated anti-fluorescein Fab2 antibody (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... washed with PBS and teased in slides coated with (3-Aminopropyl)triethoxysilane (TESPA; Sigma). Coating with TESPA was achieved by subsequently submerging glass slides in acetone for 1 min ...
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bioRxiv - Neuroscience 2020Quote: ... then lysed with either 3% SDS or RIPA lysis buffer (20-118, EMD Millipore) supplemented with protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... the plate was blocked with blocking buffer (3% BSA (Merck Millipore, MA, USA 820451) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific interactions were blocked for 1 hour with blocking solution (3% BSA, Sigma ref ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μL of azino-bis-3-ethylbenthiazoline-6-sulfonic acid (ABTS liquid substrate; Sigma) was added and incubated in the dark at room temperature for 20 minutes and 50 μL of 1% SDS was used to stop the reaction ...
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bioRxiv - Cell Biology 2021Quote: ... auxin-inducible degrons were induced with 1mM indole-3-acetic acid (IAA, Sigma-Aldrich) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... was used with CyHV-3 specific primers and probes (Sigma-Aldrich, St. Louis, USA). Further information is given in the Supporting Material (Table S3) ...